Enzyme-linked immunosorbent assay (ELISA) kits

Industrial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based

Industrial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based

allelisakits

Comparability of Industrial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based mostly Immunoassay for Detecting a Urine-Based mostly Bladder-Most cancers-Related Diagnostic Signature.

The power to precisely measure a number of proteins concurrently in a single assay has the potential to markedly enhance the effectivity of scientific assessments composed of a number of biomarkers.

We investigated the diagnostic accuracy of the 2 multiplex protein array platforms for detecting a bladder-cancer-associated diagnostic signature in samples from a cohort of 80 topics (40 with bladder most cancers). Banked urine samples collected from Kyoto and Nara Universities had been in comparison with histologically decided bladder most cancers.

 

The concentrations of the 10 proteins (A1AT; apolipoprotein E-APOE; angiogenin-ANG; carbonic anhydrase 9-CA9; interleukin 8-IL-8; matrix metalloproteinase 9-MMP-9; matrix metalloproteinase 10-MMP10; plasminogen activator inhibitor 1-PAI-1; syndecan-SDC1; and vascular endothelial development factor-VEGF) had been monitored utilizing two prototype multiplex array platforms and an enzyme-linked immunosorbent assay (ELISA) in line with the producer’s technical specs.

The vary for detecting every biomarker was improved within the multiplex assays, although the decrease restrict of quantification (LLOQ) was usually decrease within the industrial ELISA kits. The world underneath the receiver working traits (AUROC) of the prototype multiplex assays was reported to be 0.97 for the multiplex bead-based immunoassay (MBA) and 0.86 for the multiplex electrochemoluminescent assay (MEA).

The sensitivities and specificities for MBA had been 0.93 and 0.95, respectively, and for MEA had been 0.85 and 0.80, respectively. Accuracy, optimistic predictive values (PPV), and detrimental predictive values (NPV) for MBA had been 0.94, 0.95, and 0.93, respectively, and for MEA had been 0.83, 0.81, and 0.84, respectively.

Based mostly on these encouraging preliminary knowledge, we imagine {that a} multiplex protein array is a viable platform that may be utilized as an environmentally friendly and extremely correct instrument to quantitate a number of proteins inside biologic specimens.

allelisakits
allelisakits

10X ELISA Wash Buffer (125 mL)

0801060 125 mL
EUR 19

10X ELISA Wash Buffer (125 mL)

801060 125 mL
EUR 18

Wash Buffer (25X)

abx092104-30ml 30 ml
EUR 126

Block Wash Buffer

I044 1000 ml
EUR 632.4
Description: Block Wash Buffer by Cygnus Technologies is available in Europe via Gentaur.

Wash buffer concentrate (100X) for ELISA

WB-150 50 ml
EUR 139.2

PBS Wash Buffer Pack

AR1155 500mL/pack
EUR 78

TBS Wash Buffer Pack

AR0144 500mL/pack
EUR 72

AlbuVoid Wash Buffer AVWB

AVWB-1000 1000 mL
EUR 547.2
Description: Albuvoid™ Buffer

AlbuVoid Wash Buffer AVWB

AVWB-500 500 mL
EUR 294
Description: Albuvoid™ Buffer

HemoVoid Wash Buffer HVWB

HVWB-1000 1000 mL
EUR 547.2
Description: HemoVoid™ Buffer

HemoVoid Wash Buffer HVWB

HVWB-500 500 mL
EUR 294
Description: HemoVoid™ Buffer

Inclusion Body Wash Buffer

PL032-10ml 10mL
EUR 70.44

Inclusion Body Wash Buffer

PL032-5x10ml 5x10ml, 50ml
EUR 101.76

Wash Buffer (20X Concentrate)

85R-126 250 ml
EUR 236.4
Description: 10X concentrate ELISA wash buffer, ready to use

Michel's Transport Wash Buffer

MTW-10000 10 L
EUR 847.2

Michel's Transport Wash Buffer

MTW-20000 20 L
EUR 1556.4

Michel's Transport Wash Buffer

MTW500 500 ml
EUR 117.6

Michel's Transport Wash Buffer

MTW999 1000 ml
EUR 152.4

Wash Buffer Concentrate, 125ML

X007-125ML 125ML
EUR 168

Wash Buffer Concentrate, 30ML

X007-30ML 30ML
EUR 59

Wash Buffer Concentrate, 30ML

X135-30ML 30ML
EUR 59

UPCK Wash Buffer UPWB™

UPWB-1000 1000 mL
EUR 547.2
Description: UPCK™ Buffer

UPCK Wash Buffer UPWB™

UPWB-500 500 mL
EUR 294
Description: UPCK™ Buffer

Wash buffer concentrate (100X) for ELISA, 10 ml

WB-100 10 ml
EUR 123.6

EIA Wash Buffer 10x concentrate

1003-1 1 Liter
EUR 277.2

ToxOut? Rapid Removal Wash Buffer

M7947-50 each
EUR 222

Wash buffer Conc. (100X) for ELISA Kit (makes 10-Liter)

80081-K 100 ml
EUR 182.4

ToxOut? High Capacity Endotoxin Removal Wash Buffer

7946-50 each
EUR 222

Alk.Phos Enhancing Wash Buffer for rinsing IHC and

NB302 4 Liters
EUR 345
Description: Alk.Phos Enhancing Wash Buffer for rinsing IHC and Fluorescence stain, 4-Liter; Ready-To-Use

Alk.Phos Enhancing Wash Buffer for rinsing IHC and

NB302-5C 1 Liter
EUR 375
Description: Alk.Phos Enhancing Wash Buffer for rinsing IHC and Fluorescence stain, 1-Liter of 5X; Concentrate

Alk.Phos Enhancing Wash Buffer for rinsing IHC and

NB302S 1 Liter
EUR 155
Description: Alk.Phos Enhancing Wash Buffer for rinsing IHC and Fluorescence stain, 1-Liter; Ready-To-Use

Enhancing Wash Buffer for rinse/amplification of I

NB301 4 Liters
EUR 375
Description: Enhancing Wash Buffer for rinse/amplification of IHC-DAB & AEC stains and for fluorescence stains, 4-Liters; Ready-To-Use

Enhancing Wash Buffer for rinse/amplification of I

NB301-5C 1 Liter of 5X
EUR 395
Description: Enhancing Wash Buffer for rinse/amplification of IHC-DAB & AEC stains and for fluorescence stains, 1-Liter of 5X Concentrate

Enhancing Wash Buffer for rinse/amplification of I

NB301S 1 Liter
EUR 155
Description: Enhancing Wash Buffer for rinse/amplification of IHC-DAB & AEC stains and for fluorescence stains, 1-Liter; Ready-To-Use

CytoWatchâ„¢ Wash-free fluorescence cell imaging buffer *10X*

20045-10mL 10 mL
EUR 95
Description: CytoWatchâ„¢ Wash-free fluorescence cell imaging buffer *10X* is a ready-to-use buffer optimized for fluorescence cell imaging.

CytoWatchâ„¢ Wash-free fluorescence cell imaging buffer *10X*

20046-100mL 100 mL
EUR 295
Description: CytoWatchâ„¢ Wash-free fluorescence cell imaging buffer *10X* is a ready-to-use buffer optimized for fluorescence cell imaging.

Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers

5730 EACH
EUR 955.2

West-Ezier Washing Buffer(10X)

W3873-050 500ml
EUR 141.6

Corning 3D Clear Washing Buffer 10X - 70mL

5740 EACH
EUR 172.8

Corning 3D Clear Washing Buffer 10X - 200mL

5741 EACH
EUR 318

ELISA Binding Buffer

EBB125 125 ml
EUR 76.8

ELISA Binding Buffer

EBB500 500 ml
EUR 104.4

ELISA Binding Buffer

EBB999 1000 ml
EUR 129.6

EIA Buffer for ELISA

GR103069 100 mL
EUR 69

T-Pro Washing buffer in PBS and Tween-20 (10X)

JB09-I003 500ml/BT
EUR 172.8

T-Pro Washing buffer in TBS and Tween-20 (10X)

JB09-I004 500ml/BT
EUR 172.8

ELISA Assay Buffer, 20x

GR103001 1 L
EUR 89

ELISA Assay Buffer, 80x

GR103002 1 L
EUR 159

Blocking Buffer for ELISA

abx090807-500ml 500 ml
EUR 460.8

High Performance ELISA Buffer

85R-105 55 ml
EUR 379.2
Description: High Performance ELISA Buffer for high sensitivity ELISA assays

2 x Lysis Buffer for ELISA Kit

GR103073 20 mL
EUR 30

CSB; Cytokine Stabilization Buffer ELISA

CT373 5 ml
EUR 114

Borax Buffer 0.00996M

BOR0091 1L
EUR 76.8

TT Buffer (Tris-Tricine buffer) Primix powder

TD8133 1PK, 10L
EUR 91.32

ADA buffer

AD0003 25g
EUR 77.75

PBS Buffer

RM00012 2L
EUR 122.4

RIPA Buffer

2114-100 each
EUR 189.6

RIPA Buffer

2114-500 each
EUR 555.6

RIPA Buffer

GR103019 50 mL
EUR 59

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-050 500ml
EUR 96

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-100 2X500ml
EUR 124.8

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-101 1L
EUR 114

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-200 4X500ml
EUR 153.6

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-201 2X1L
EUR 153.6

10X Tris-Glycine Native Buffer (Transfer buffer)

T8052-401 4X1L
EUR 198

Lysis Buffer

abx098984-LysisBuffer120ml Lysis Buffer 1 (20 ml)
EUR 184.8

Lysis Buffer

abx098984-LysisBuffer3100ml Lysis Buffer 3 (100ml)
EUR 276

Lysis Buffer

abx098984-LysisBuffer420ml Lysis Buffer 4 (20 ml)
EUR 184.8

Anode Buffer

GR103036 1 L
EUR 89

Assay Buffer, 225ML

X040-225ML 225ML
EUR 126

10xTaq Buffer

PCRB60 4x1.5ml, 6ml
EUR 70.44

Coating Buffer

abx098970-1vial 1 vial
EUR 184.8

Binding Buffer

BB10X-50ML 50 ML
EUR 136.44

Tricine buffer

20-abx082551
  • EUR 260.40
  • EUR 226.80
  • 100 g
  • 25 g

Binding Buffer

abx290019-50ml 50 ml
EUR 276

Cathode Buffer

GR103037 1 L
EUR 109

PCR Buffer I, Mg Free 10X, Ammonium Rxn Buffer

42-300 3 x 1.5ml/Unit
EUR 285

ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml

9068 100 ml
EUR 155.7
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer

Blocking Buffer

abx098972-1vial 1 vial
EUR 184.8

Dilution Buffer

1066-100 each
EUR 183.6

Dilution Buffer

1066-400 each
EUR 314.4

Phosphate Buffer

PB1 1L
EUR 118.81

Stripping Buffer

GR103020 500 mL
EUR 89

 

Comparability of Fast Anti-HCV Multi-sure Equipment with Gold Customary ELISA.

To match the diagnostic yield of Multi-sure speedy HCV (hepatitis C virus) equipment with ELISA.Comparative examine.Pakistan Well being Analysis Council, specialised analysis heart for gastroenterology and hepatology, from August 2016 to January 2017.

A modified speedy anti-HCV equipment was in contrast with ELISA. This speedy equipment is multi-parameter qualitative immune chromatographic equipment for the in-vitro detection of antibodies to HCV in human blood. Sufferers who got here to PHRC, had been examined utilizing anti-HCV ELISA, and their take a look at was run concurrently on multi-sure HCV speedy equipment had been included within the examine. Every optimistic and detrimental pattern was included on this examine. SPSS software program was tailored for knowledge evaluation.

A complete of 420 samples had been collected. Amongst them, 255 (61%) had been of male and 165 (39%) had been of feminine sufferers. Imply age was 35 ± 14.33 years. All of the samples run for anti-HCV on ELISA had been additionally run on multi-sure speedy equipment.

It’s evident that 22.4% had been reactive on ELISA and 23.6% had been reactive on speedy equipment, whereas 75.5% had been non-reactive on ELISA and 68.1% had been non-reactive on speedy equipment. Borderline optimistic outcomes had been seen in 2.1% on ELISA and 5.0% on speedy equipment. Sensitivity of speedy equipment was 87.2%, specificity 89.3% with 82.8% optimistic predictive worth and 98.9% detrimental predictive worth.

Multi-sure equipment confirmed considerably, much less non-reactive and extra borderline outcomes as in comparison with ELISA. Comparability of multi-sure speedy equipment with ELISA confirmed that core antibody can be utilized as an alternate marker for ELISA. Different non-structural proteins together with NS3, NS4 and NS5 had been discovered to be much less important. So, it’s concluded that this speedy equipment is probably not really helpful in its place of ELISA, apart from locations the place ELISA will not be obtainable.

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060 50 ml
EUR 242.4

General Blocking Buffer-BB1

KF17337 500 ml
EUR 277.2

Neptune Blocking Buffer-BB2

KF17338 500 ml
EUR 292.8

PBS Blocking buffer with BSA

SB0626 1pk, 1L
EUR 100.72

TBS blocking buffer with BSA

SD8113 1pk, 1L
EUR 100.72

3 Blocking Buffer Sample Pack

KF17340 3 x 100 ml
EUR 246

ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml

9068 100 ml
EUR 155.7
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer

EZBlock? T20 (TBS) Blocking Buffer

2140-1000 each
EUR 261.6

EZBlock? T20 (TBS) Blocking Buffer

2140-200 each
EUR 144

EZBlock? T20 (PBS) Blocking Buffer

2143-1000 each
EUR 261.6

EZBlock? T20 (PBS) Blocking Buffer

2143-200 each
EUR 144

10 x Universal Blocking Buffer

GR103005 200 mL
EUR 129

TrueBlack WB Blocking Buffer Kit

23013 1kit
EUR 416.4
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit

23013-T 1kit
EUR 150
Description: Minimum order quantity: 1 unit of 1kit

Corning 3D Clear Blocking Buffer - 100mL

5737 EACH
EUR 325.2

Blocking Buffer for ICC and IHC

SF40011 10 ml
EUR 153.6

West-Ezier Super Blocking Buffer

W3700-010 100ml
EUR 116.4

West-Ezier Super Blocking Buffer

W3700-050 500ml
EUR 198

West-Ezier Super Blocking Buffer

W3700-100 1L
EUR 295.2

Claudin Domain Containing 1 Blocking Peptide

33R-9186 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CLDND1 antibody, catalog no. 70R-6340

Blocking Buffer and Coating Stabilizer

abx090811-1000ml 1000 ml
EUR 627.6

T-Pro Fast Blocking Buffer (in PBS)

JK92-W001 500ml/BT
EUR 224.4

T-Pro Fast Blocking Buffer (in TBS)

JK92-W002 500ml/BT
EUR 224.4

Corning 3D Clear Blocking Buffer - 30mL

5736 EACH
EUR 120

Casein-based PBS Blocking Buffer Pack

AR0182 500mL/Pack
EUR 84

Casein-based TBS Blocking Buffer Pack

AR0183 500mL/Pack
EUR 84

PBS Blocking buffer with non fat milk

SB0625 1pk, 1L
EUR 80.88

EZBlock? Blocking Buffer and Signal Enhancer

M4104-100 each
EUR 183.6

EZBlock? Blocking Buffer and Signal Enhancer

M4104-500 each
EUR 430.8

Blocking Buffer for Lateral Flow (PBS based)

abx090808-500ml 500 ml
EUR 460.8

Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers

5730 EACH
EUR 955.2

Blocking Buffer 3 (Multiple PARP Assay Kits)

79743 25 ml x 2
EUR 105
Description: This Blocking Buffer 3 is optimized for minimizing background in multiple PARP BPS Bioscience Assay Kits. _x000D__x000D_

T-Pro Protein Free Blocking Buffer (in PBS)

JK92-W003 500ml/BT
EUR 224.4

T-Pro Protein Free Blocking Buffer (in TBS)

JK92-W004 500ml/BT
EUR 224.4

Cyclin N-Terminal Domain Containing 1 Blocking Peptide

33R-7660 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CNTD1 antibody, catalog no. 70R-4549

Blocking Buffer 2 (BPS Immunotherapy Assay Kits)

79728 50 ml
EUR 110

Blocking Buffer 1 (Various Epigenetics Assay Kits)

79556 50 ml
EUR 100
Description: This Blocking Buffer 1 is optimized for minimizing background in various BPS Bioscience assay kits. The BPS Bioscience Blocking Buffer improves S/B ratio of HMTs NSD2 and SetDB2 ELISAs with respect to competitor blocking buffer by both decreasing background (0% Activity control, no enzyme) and increasing assay signal (100% Activity control, enzyme without inhibitor).

Blocking Buffer 4 (Multiple Epigenetics Assay Kits)

52100 50 ml
EUR 95
Description: This Blocking Buffer 4 is optimized for minimizing background in various chemiluminescent methyltransferase, demethylase, and TET assays.

Human WW and C2 domain containing 1 (WWC1) Control/blocking peptide

AB-23075-P 100ug
EUR 196.8

Human WW and C2 domain containing 1 (WWC1) Control/blocking peptide

AB-23076-P 100ug
EUR 196.8

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-050 500ml
EUR 144

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-100 2x500ml
EUR 176.4

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST

W3710-010 100ml
EUR 111.6

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST

W3710-050 500ml
EUR 165.6

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST

W3710-100 1L
EUR 218.4

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-010 100ml
EUR 157.2

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-050 500ml
EUR 225.6

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-100 1L
EUR 336

Mouse zinc finger, BED domain containing 3 (ZBED3) Control/blocking peptide

AB-23250-P 100ug
EUR 196.8

West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST

W3711-010 100ml
EUR 116.4

West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST

W3711-050 500ml
EUR 174

West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST

W3711-100 1L
EUR 230.4

Mouse NLR family, pyrin domain containing 4F (NLRP4F) Control/blocking peptide

AB-23251-P 100ug
EUR 196.8

West-Ezier Rapid Blocking Buffer, Ready-to-use, 5 Min

W3871-050 500ml
EUR 230.4

Human Pleckstrin homology domain containing, family A member 7 (PLEKHA7) Control/blocking peptide

AB-23061-P 100ug
EUR 196.8

Antigen blocking and stabilizing buffer for ELISA plates (non-bovine protein formulation) (for 10-20 plates)

80065 100 ml
EUR 226.8

A kit containing all affinity purification buffers for 10-20 antibody purifications

110300-AF 1 kit
EUR 343.2

Real Time PCR mastermix 2x, (No ROX), 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400RF each
EUR 261.6

Block Wash Buffer

I044 1000 ml
EUR 632.4
Description: Block Wash Buffer by Cygnus Technologies is available in Europe via Gentaur.

Buffer Saver Blocks for MSMAXI saves 450ml of buffer

MSMAXIBSB EACH
EUR 104.4

Buffer Saver Blocks for MSCHOICE saves 190ml of buffer

MSCHOICEBSB EACH
EUR 91.2

Real-Time PCR Mastermix 2x, Regular Level of Rox, 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400 each
EUR 261.6

Real-Time PCR Mastermix 2x, Low Level of Rox , 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400LR each
EUR 261.6

buffer saving blocks pk/2

EHS1100-BUFSAVE ea
EUR 67.2

buffer saving blocks pk/2

EHS1200-BUFSAVE ea
EUR 78

buffer saving blocks pk/2

EHS1400-BUFSAVE ea
EUR 108

buffer saving blocks pk/2

EHS1500-BUFSAVE ea
EUR 118.8

SYN3 Blocking Peptide

33R-3176 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of SYN3 antibody, catalog no. 70R-10563

SYN2 Blocking Peptide

DF7862-BP 1mg
EUR 234

Dog LMBR1 domain containing 1 ELISA kit

E08L1189    -192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine LMBR1 domain containing 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog LMBR1 domain containing 1 ELISA kit

E08L1189    -48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine LMBR1 domain containing 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog LMBR1 domain containing 1 ELISA kit

E08L1189    -96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine LMBR1 domain containing 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig LMBR1 domain containing 1 ELISA kit

E07L1189    -192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine LMBR1 domain containing 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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