Enzyme-linked immunosorbent assay (ELISA) kits

Industrial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based

Industrial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based

allelisakits

Comparability of Industrial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based mostly Immunoassay for Detecting a Urine-Based mostly Bladder-Most cancers-Related Diagnostic Signature.

The power to precisely measure a number of proteins concurrently in a single assay has the potential to markedly enhance the effectivity of scientific assessments composed of a number of biomarkers.

We investigated the diagnostic accuracy of the 2 multiplex protein array platforms for detecting a bladder-cancer-associated diagnostic signature in samples from a cohort of 80 topics (40 with bladder most cancers). Banked urine samples collected from Kyoto and Nara Universities had been in comparison with histologically decided bladder most cancers.

 

The concentrations of the 10 proteins (A1AT; apolipoprotein E-APOE; angiogenin-ANG; carbonic anhydrase 9-CA9; interleukin 8-IL-8; matrix metalloproteinase 9-MMP-9; matrix metalloproteinase 10-MMP10; plasminogen activator inhibitor 1-PAI-1; syndecan-SDC1; and vascular endothelial development factor-VEGF) had been monitored utilizing two prototype multiplex array platforms and an enzyme-linked immunosorbent assay (ELISA) in line with the producer’s technical specs.

The vary for detecting every biomarker was improved within the multiplex assays, although the decrease restrict of quantification (LLOQ) was usually decrease within the industrial ELISA kits. The world underneath the receiver working traits (AUROC) of the prototype multiplex assays was reported to be 0.97 for the multiplex bead-based immunoassay (MBA) and 0.86 for the multiplex electrochemoluminescent assay (MEA).

The sensitivities and specificities for MBA had been 0.93 and 0.95, respectively, and for MEA had been 0.85 and 0.80, respectively. Accuracy, optimistic predictive values (PPV), and detrimental predictive values (NPV) for MBA had been 0.94, 0.95, and 0.93, respectively, and for MEA had been 0.83, 0.81, and 0.84, respectively.

Based mostly on these encouraging preliminary knowledge, we imagine {that a} multiplex protein array is a viable platform that may be utilized as an environmentally friendly and extremely correct instrument to quantitate a number of proteins inside biologic specimens.

allelisakits
allelisakits

20 x ELISA Wash Buffer

GR103014-20 1 L
EUR 189.6

40 x ELISA Wash Buffer

GR103014-40 1 L
EUR 238.8

Wash Buffer (20X Concentrate)

85R-126 250 ml
EUR 236.4
Description: 10X concentrate ELISA wash buffer, ready to use

TBS Wash Buffer Pack

AR0144 500mL/pack
EUR 72

PBS Wash Buffer Pack

AR1155 500mL/pack
EUR 78

AlbuVoid Wash Buffer AVWB

AVWB-1000 1000 mL
EUR 547.2
Description: Albuvoid™ Buffer

AlbuVoid Wash Buffer AVWB

AVWB-500 500 mL
EUR 294
Description: Albuvoid™ Buffer

Michel's Transport Wash Buffer

MTW-10000 10 L
EUR 847.2

Michel's Transport Wash Buffer

MTW-20000 20 L
EUR 1556.4

Michel's Transport Wash Buffer

MTW500 500 ml
EUR 117.6

Michel's Transport Wash Buffer

MTW999 1000 ml
EUR 152.4

HemoVoid Wash Buffer HVWB

HVWB-1000 1000 mL
EUR 547.2
Description: HemoVoid™ Buffer

HemoVoid Wash Buffer HVWB

HVWB-500 500 mL
EUR 294
Description: HemoVoid™ Buffer

Inclusion Body Wash Buffer

PL032-10ml 10mL
EUR 70.44

Inclusion Body Wash Buffer

PL032-5x10ml 5x10ml, 50ml
EUR 101.76

Wash Buffer Concentrate, 125ML

X007-125ML 125ML
EUR 232.8

Wash Buffer Concentrate, 30ML

X007-30ML 30ML
EUR 146.4

Wash Buffer Concentrate, 30ML

X135-30ML 30ML
EUR 146.4

10X ELISA Wash Buffer (125 mL)

0801060 125 mL
EUR 78.72
Description: Please contact Gentaur in order to receive the datasheet of the product.

Wash buffer concentrate (20X) for ELISA

80080 100 ml
EUR 196.8

Wash buffer concentrate (100X) for ELISA

WB-150 50 ml
EUR 139.2

10X ELISA Wash Buffer (125 mL)

801060 125 mL
EUR 18

EIA Wash Buffer 10x concentrate

1003-1 1 Liter
EUR 277.2

ToxOut? Rapid Removal Wash Buffer

M7947-50
EUR 222

UPCK Wash Buffer UPWB™

UPWB-1000 1000 mL
EUR 547.2
Description: UPCK™ Buffer

UPCK Wash Buffer UPWB™

UPWB-500 500 mL
EUR 294
Description: UPCK™ Buffer

Wash buffer concentrate (100X) for ELISA, 10 ml

WB-100 10 ml
EUR 123.6

ToxOut? High Capacity Endotoxin Removal Wash Buffer

7946-50
EUR 222

Wash buffer Conc. (100X) for ELISA Kit (makes 10-Liter)

80081-K 100 ml
EUR 182.4

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 1
EUR 242.4

Wash bottle assembly

MW9600-WSHB 1 each
EUR 164.8

ELISA Binding Buffer

EBB125 125 ml
EUR 76.8

ELISA Binding Buffer

EBB500 500 ml
EUR 104.4

ELISA Binding Buffer

EBB999 1000 ml
EUR 129.6

Diversey Soft Care Wash Mild Hand Wash H2 500mL

SAF5380 PK10
EUR 133.38

Alk.Phos Enhancing Wash Buffer for rinsing IHC and Fluorescence, 1 liter of 5X Concentrate

NB302-5C 1 Liter
EUR 582

Alk.Phos Enhancing Wash Buffer For rinsing IHC and Fluorescence, 1 Liter Ready-To-Use

NB302S 1 Liter
EUR 247.2

ELISA PLATE WASHER : MICRO WASH 1100

1100 1 unit
EUR 2403.6

TBS Wash Concentrate, 20X

F004 50 ml
EUR 216
Description: TBS Wash Concentrate, 20X by Cygnus Technologies is available in Europe via Gentaur.

TBS Wash Concentrate, 20X

F004-1000 1000 ml
EUR 483.6
Description: TBS Wash Concentrate, 20X by Cygnus Technologies is available in Europe via Gentaur.

Block/Wash 20x Concentrate

F062 50 ml
EUR 234
Description: Block/Wash 20x Concentrate by Cygnus Technologies is available in Europe via Gentaur.

Block/Wash 20x Concentrate

F062-100 100 ml
EUR 355.2
Description: Block/Wash 20x Concentrate by Cygnus Technologies is available in Europe via Gentaur.

Block/Wash 20X Concentrate

F062-1000 1000 ml
EUR 493.2
Description: Block/Wash 20X Concentrate by Cygnus Technologies is available in Europe via Gentaur.

Wash Bottle Nalge 1Ltr

2402-1000 PK2
EUR 23.94

500mL Wash Bottle Acetone

BOT0038 PK5
EUR 38.38

500mL Wash Bottle Methanol

BOT0302 PK5
EUR 36.71

500mL Wash Bottle Isopropanol

BOT0304 PK5
EUR 36.71

500mL Wash Bottle Ethanol

BOT0306 PK5
EUR 36.71

Wash Solution Concentrate (50X)

WSC999 1000 ml
EUR 178.8

Eye Wash Cabinet Complete

SAF2420 EACH
EUR 32.15

Wash Bottle LDPE 250ml

BOT9000 EACH
EUR 6.73

Wash Bottle LDPE 500ml

BOT9002 EACH
EUR 5.59

Ammonia Monitor Wash Solution

WASOL25 2.5L
EUR 69.54

Alk.Phos IHC Enhancing Wash Buffer for rinsing IHC and Fluorescence, 4 Liter Ready-To-Use

NB302 4 Liters
EUR 512.4

Blocking Buffer for ELISA

abx090807-500ml 500 ml
EUR 460.8

High Performance ELISA Buffer

85R-105 55 ml
EUR 379.2
Description: High Performance ELISA Buffer for high sensitivity ELISA assays

ELISA Assay Buffer, 20x

GR103001 1 L
EUR 106.8

ELISA Assay Buffer, 80x

GR103002 1 L
EUR 190.8

EIA Buffer for ELISA

GR103069 100 mL
EUR 82.8

RIPA Buffer

2114-100
EUR 189.6

RIPA Buffer

2114-500
EUR 555.6

Dilution Buffer

1066-100
EUR 183.6

Dilution Buffer

1066-400
EUR 314.4

Tricine buffer

20-abx082551
  • EUR 260.40
  • EUR 226.80
  • 100 g
  • 25 g

Coating Buffer

abx098970-1vial 1 vial
EUR 184.8

Blocking Buffer

abx098972-1vial 1 vial
EUR 184.8

Lysis Buffer

abx098984-LysisBuffer120ml Lysis Buffer 1 (20 ml)
EUR 184.8

Lysis Buffer

abx098984-LysisBuffer3100ml Lysis Buffer 3 (100ml)
EUR 276

Lysis Buffer

abx098984-LysisBuffer420ml Lysis Buffer 4 (20 ml)
EUR 184.8

Binding Buffer

abx290019-50ml 50 ml
EUR 276

Binding Buffer

BB10X-50ML 50 ML
EUR 136.44

ADA buffer

AD0003 25g
EUR 77.75

RIPA Buffer

GR103019 50 mL
EUR 70.8

Stripping Buffer

GR103020 500 mL
EUR 106.8

Anode Buffer

GR103036 1 L
EUR 106.8

Cathode Buffer

GR103037 1 L
EUR 130.8

10xTaq Buffer

PCRB60 4x1.5ml, 6ml
EUR 70.44

PBS Buffer

RM00012 2L
EUR 122.4

Phosphate Buffer

PB1 1L
EUR 94.62

WASH BOTTLE 1 L, LDPE

1300P-1L 12/pk
EUR 122.4
Description: Reusable Plastics; Resusable Bottles

WASH BOTTLE 250 ML, LDPE

1300P-250 12/pk
EUR 84
Description: Reusable Plastics; Resusable Bottles

WASH BOTTLE 500 ML, LDPE

1300P-500 12/pk
EUR 97.2
Description: Reusable Plastics; Resusable Bottles

FINAL WASH SOLUTION/CULTURE MEDIUM

99-785-CV 500 mL/pk
EUR 196.8
Description: Specialty Media; Islet products

 

Comparability of Fast Anti-HCV Multi-sure Equipment with Gold Customary ELISA.

To match the diagnostic yield of Multi-sure speedy HCV (hepatitis C virus) equipment with ELISA.Comparative examine.Pakistan Well being Analysis Council, specialised analysis heart for gastroenterology and hepatology, from August 2016 to January 2017.

A modified speedy anti-HCV equipment was in contrast with ELISA. This speedy equipment is multi-parameter qualitative immune chromatographic equipment for the in-vitro detection of antibodies to HCV in human blood. Sufferers who got here to PHRC, had been examined utilizing anti-HCV ELISA, and their take a look at was run concurrently on multi-sure HCV speedy equipment had been included within the examine. Every optimistic and detrimental pattern was included on this examine. SPSS software program was tailored for knowledge evaluation.

A complete of 420 samples had been collected. Amongst them, 255 (61%) had been of male and 165 (39%) had been of feminine sufferers. Imply age was 35 ± 14.33 years. All of the samples run for anti-HCV on ELISA had been additionally run on multi-sure speedy equipment.

It’s evident that 22.4% had been reactive on ELISA and 23.6% had been reactive on speedy equipment, whereas 75.5% had been non-reactive on ELISA and 68.1% had been non-reactive on speedy equipment. Borderline optimistic outcomes had been seen in 2.1% on ELISA and 5.0% on speedy equipment. Sensitivity of speedy equipment was 87.2%, specificity 89.3% with 82.8% optimistic predictive worth and 98.9% detrimental predictive worth.

Multi-sure equipment confirmed considerably, much less non-reactive and extra borderline outcomes as in comparison with ELISA. Comparability of multi-sure speedy equipment with ELISA confirmed that core antibody can be utilized as an alternate marker for ELISA. Different non-structural proteins together with NS3, NS4 and NS5 had been discovered to be much less important. So, it’s concluded that this speedy equipment is probably not really helpful in its place of ELISA, apart from locations the place ELISA will not be obtainable.

Blocking Buffer, 10X, 250ML

X109-250ML 250ML
EUR 430.8

Blocking Buffer, 10X, 25ML

X109-25ML 25ML
EUR 146.4

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1 1
EUR 242.4

EZBlock? T20 (TBS) Blocking Buffer

2140-1000
EUR 261.6

EZBlock? T20 (TBS) Blocking Buffer

2140-200
EUR 144

EZBlock? T20 (PBS) Blocking Buffer

2143-1000
EUR 261.6

EZBlock? T20 (PBS) Blocking Buffer

2143-200
EUR 144

TrueBlack WB Blocking Buffer Kit

23013 1kit
EUR 416.4
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit

23013-T 1kit
EUR 150
Description: Minimum order quantity: 1 unit of 1kit

Blocking Buffer and Coating Stabilizer

abx090811-1000ml 1000 ml
EUR 627.6

3 Blocking Buffer Sample Pack

KF17340 3 x 100 ml
EUR 246

10 x Universal Blocking Buffer

GR103005 200 mL
EUR 154.8

TBS blocking buffer with BSA

SD8113 1pk, 1L
EUR 100.72

PBS Blocking buffer with BSA

SB0626 1pk, 1L
EUR 100.72

West-Ezier Super Blocking Buffer

W3700-010 100ml
EUR 116.4

West-Ezier Super Blocking Buffer

W3700-050 500ml
EUR 198

West-Ezier Super Blocking Buffer

W3700-100 1L
EUR 295.2

ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml

9068 100 ml
EUR 155.7
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060 50 ml
EUR 242.4

Casein-based PBS Blocking Buffer Pack

AR0182 500mL/Pack
EUR 84

Casein-based TBS Blocking Buffer Pack

AR0183 500mL/Pack
EUR 84

EZBlock? Blocking Buffer and Signal Enhancer

M4104-100
EUR 183.6

EZBlock? Blocking Buffer and Signal Enhancer

M4104-500
EUR 430.8

Blocking Buffer for ICC and IHC

SF40011 10 ml
EUR 153.6

Corning 3D Clear Blocking Buffer - 30mL

5736 EACH
EUR 108.3

Corning 3D Clear Blocking Buffer - 100mL

5737 EACH
EUR 294.12

Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers

5730 EACH
EUR 864.12

Blocking Buffer for Lateral Flow (PBS based)

abx090808-500ml 500 ml
EUR 460.8

T-Pro Fast Blocking Buffer (in PBS)

JK92-W001 500ml/BT
EUR 224.4

T-Pro Fast Blocking Buffer (in TBS)

JK92-W002 500ml/BT
EUR 224.4

PBS Blocking buffer with non fat milk

SB0625 1pk, 1L
EUR 80.88

ELISA Binding Buffer

EBB125 125 ml
EUR 76.8

ELISA Binding Buffer

EBB500 500 ml
EUR 104.4

ELISA Binding Buffer

EBB999 1000 ml
EUR 129.6

T-Pro Protein Free Blocking Buffer (in PBS)

JK92-W003 500ml/BT
EUR 224.4

T-Pro Protein Free Blocking Buffer (in TBS)

JK92-W004 500ml/BT
EUR 224.4

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-010 100ml
EUR 157.2

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-050 500ml
EUR 225.6

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-100 1L
EUR 336

High Performance ELISA Buffer

85R-105 55 ml
EUR 379.2
Description: High Performance ELISA Buffer for high sensitivity ELISA assays

ELISA Assay Buffer, 20x

GR103001 1 L
EUR 106.8

ELISA Assay Buffer, 80x

GR103002 1 L
EUR 190.8

EIA Buffer for ELISA

GR103069 100 mL
EUR 82.8

Sandwich Elisa Wash Buffer

RKB0001 20ml
EUR 142.8

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-050 500ml
EUR 144

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-100 2x500ml
EUR 176.4

Wash Buffer

1210-200
EUR 235.2

RIPA Buffer

2114-100
EUR 189.6

RIPA Buffer

2114-500
EUR 555.6

Dilution Buffer

1066-100
EUR 183.6

Dilution Buffer

1066-400
EUR 314.4

Tricine buffer

20-abx082551
  • EUR 260.40
  • EUR 226.80
  • 100 g
  • 25 g

Wash Buffer

abx098952-20ml 20 ml
EUR 109.2

Coating Buffer

abx098970-1vial 1 vial
EUR 184.8

Lysis Buffer

abx098984-LysisBuffer120ml Lysis Buffer 1 (20 ml)
EUR 184.8

Lysis Buffer

abx098984-LysisBuffer3100ml Lysis Buffer 3 (100ml)
EUR 276

Lysis Buffer

abx098984-LysisBuffer420ml Lysis Buffer 4 (20 ml)
EUR 184.8

Binding Buffer

abx290019-50ml 50 ml
EUR 276

Wash Buffer

abx293002-30ml 30 ml
EUR 126

Binding Buffer

BB10X-50ML 50 ML
EUR 136.44

ADA buffer

AD0003 25g
EUR 77.75

Wash Buffer

KF17356 500 ml
EUR 214.8

RIPA Buffer

GR103019 50 mL
EUR 70.8

Stripping Buffer

GR103020 500 mL
EUR 106.8

Anode Buffer

GR103036 1 L
EUR 106.8

Cathode Buffer

GR103037 1 L
EUR 130.8

10xTaq Buffer

PCRB60 4x1.5ml, 6ml
EUR 70.44

PBS Buffer

RM00012 2L
EUR 122.4

Phosphate Buffer

PB1 1L
EUR 94.62

CSB; Cytokine Stabilization Buffer ELISA

CT373 5 ml
EUR 114

10 x ELISA Wash Buffer

GR103014 1 L
EUR 117.6

20 x ELISA Wash Buffer

GR103014-20 1 L
EUR 189.6

40 x ELISA Wash Buffer

GR103014-40 1 L
EUR 238.8

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