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ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1) Organism: Rhesus monkey (Simian)

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[#E90087Si] ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1) Organism: Rhesus monkey (Simian)

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E90087Si | ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1) Organism: Rhesus monkey (Simian), 96T
More informations about ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1) Organism: Rhesus monkey (Simian) in Antibody-antibodies.com

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(1) Modulation the crosstalk between tumor-associated macrophages and non-small cell lung cancer to inhibit tumor migration and invasion by ginsenoside Rh2.[TOP]

Pubmed ID :29783929
Publication Date : //
Tumor-associated macrophages (TAMs) play a critical role in modulating the tumor microenvironment and promote tumor metastases. Our studies have demonstrated that ginsenoside Rh2 (G-Rh2), a monomeric compound extracted from ginseng, is a promising anti-tumor agent in lung cancer cells. However, it remains unclear whetherG-Rh2 can modulate the differentiation of TAMs and its interaction with tumor microenvironment. In this study, we investigated how G-Rh2 regulates the phenotype of macrophages and affects the migration of non-small cell lung cancer (NSCLC) cells.

Authors : Li Honglin, Huang Nan, Zhu Weikang, Wu Jianchun, Yang Xiaohui, Teng Wenjing, Tian Jianhui, Fang Zhihong, Luo Yingbin, Chen Min, Li Yan,



(2) Puerarin prevents vascular endothelial injury through suppression of NF-κB activation in LPS-challenged human umbilical vein endothelial cells.[TOP]

Pubmed ID :29775893
Publication Date : //
In the present study, we aimed to explore the effects of puerarin on vascular endothelial cell injury induced by lipopolysaccharide (LPS) and its underlying mechanisms.

Authors : Deng Hua-Fei, Wang Sha, Li Lian, Zhou Qin, Guo Wan-Bei, Wang Xiao-Li, Liu Mei-Dong, Liu Ke, Xiao Xian-Zhong,



(3) Hydrogen sulfide exposure induces NLRP3 inflammasome-dependent IL-1β and IL-18 secretion in human mononuclear leukocytes .[TOP]

Pubmed ID :29744188
Publication Date : //
The aim was to investigate if hydrogen sulfide (HS) induces the formation of the NLRP3 inflammasome and subsequent IL-1β and IL-18 secretion in human peripheral blood mononuclear cells (PBMCs) and in the human monocyte cell line THP1. Bacterial production of HS has been suggested to participate in the inflammatory host response in periodontitis pathogenesis. HS is a toxic gas with pro-inflammatory properties. It is produced by bacterial degradation of sulfur-containing amino acids, for example, cysteine. We hypothesize that HS affects the inflammatory host response by inducing formation of the NLRP3 inflammasome and thereby causes the secretion of IL-1ß and IL-18. PBMCs from eight healthy blood donors, the human monocyte cell line THP1 Null, and two variants of the THP1 cell line unable to form the NLRP3 inflammasome were cultured in the presence or absence of 1 mM sodium hydrosulfide (NaHS) in 24-well plates at 37°C for 24 hr. Supernatants were collected and the IL-1β and IL-18 concentrations were measured with DuoSet ELISA Development kit. PBMCs exposed to NaHS produced more IL-1ß and IL-18 than unexposed control cells ( = .023 and  = .008, respectively). An increase of extracellular potassium ions (K) inhibited the secretion of IL-1ß and IL-18 ( = .008). Further, NaHS triggered the secretion of IL-1ß and IL-18 in human THP1-Null monocytes ( = .0006 and  = .002, respectively), while the NaHS-dependent secretion was reduced in the monocyte cell lines unable to form the NLRP3 inflammasome. Hence, the results suggest that NaHS induces the formation of the NLRP3 inflammasome and thus the secretion of IL-1ß and IL-18. Enhanced NLRP3 inflammasome-dependent secretion of IL-1β and IL-18 in human mononuclear leukocytes exposed to NaHS is reported. This may be a mode for HS to contribute to the inflammatory host response and pathogenesis of periodontal disease.

Authors : Basic Amina, Alizadehgharib Sara, Dahlén Gunnar, Dahlgren Ulf,



(4) Circulating soluble RAGE and cell surface RAGE on peripheral blood mononuclear cells in healthy children.[TOP]

Pubmed ID :29723156
Publication Date : //
The receptor for advanced glycation end products (RAGE) has a critical role in the pathogenesis of inflammation. In healthy children, its basal expression on the peripheral blood mononuclear cell (PBMC) and the basal circulating soluble RAGE (sRAGE) levels are unknown. The aim of this study was to describe both.

Authors : García-Salido Alberto, Melen Gustavo, Gómez-Piña Vanesa, Oñoro-Otero Gonzalo, Serrano-González Ana, Casado-Flores Juan, Ramírez Manuel,



(5) Association of Peripheral Monocyte Count with Soluble P-Selectin and Advanced Stages in Nasopharyngeal Carcinoma.[TOP]

Pubmed ID :29692812
Publication Date : //
Inflammation is widely recognized to play an important role in cancer progression and is related to thrombosis. Soluble P-selectin (sP-selectin) is one of several biomarkers that may be predictive of thrombosis in cancer. This study aimed to investigate the correlation between monocyte count and sP-selectin in various stages of nasopharyngeal carcinoma.

Authors : Komala Adi Surya, Rachman Andhika,



(6) Different profile of cytokine production in patients with systemic sclerosis and association with clinical manifestations.[TOP]

Pubmed ID :29601941
Publication Date : //
Immune dysregulation is a central process in the pathogenesis of systemic sclerosis (SSc). Cytokines produced by lymphocytes and monocytes are important mediators and induce tissue damage, recruit additional inflammatory cells, and promote extracellular matrix production and fibrosis. In the present research, we aimed to study the associations between levels of cytokines in serum and culture supernatants from peripheral blood mononuclear cells (PBMCs) and clinical manifestations in SSc patients. Serum samples were obtained from 56 SSc patients and 56 unrelated age- and gender-matched healthy individuals. Resting and anti-CD3/CD28-stimulated PBMC cultures were obtained from 19 SSc patients and 8 healthy controls. IL-2, IL-4, IL-6, IL-10, IL-17A, TNF, and IFN-γ levels were measured by ELISA or CBA. Serum cytokines, except IL-17A, were below the kit detection limit in most of the patients and controls. In unstimulated PBMC, the production of TNF(p = 0.004), IL-10(p = .048), IL-2(p < 0.001), and IL-6 (p = 0.01) was higher in SSc patients than in healthy controls. After anti-CD3/CD28 stimulation, scleroderma PBMCs had lower concentrations of TNF(p = 0.009), IL-10(p = .018), and IL-2(p = .002) than HC. In unstimulated PBMC, IL-2 concentration was higher in patients with esophageal dysmotility (p = 0.04), and IL-10 levels had a positive correlation with modified Rodnan score (p = 0.03). After anti-CD3/CD28 stimulation, higher levels of IL-2 and IL-4 were observed in SSc patients with lung fibrosis (p = 0.01 and 0.006, respectively), and higher levels of IL-10 (p = 0.04) and IL-4 (p = 0.04) in patients with digital ulcers. In conclusion, SSc patients have a different profile of cytokine production and this was associated with clinical manifestations.

Authors : Dantas Andréa Tavares, Almeida Anderson Rodrigues de, Sampaio Maria Clara Pinheiro Duarte, Cordeiro Marina Ferraz, Oliveira Priscilla Stela Santana de, Mariz Henrique de Ataíde, Pereira Michelly Cristiny, Rego Moacyr Jesus Barreto de Melo, Pitta Ivan da Rocha, Duarte Angela Luzia Branco Pinto, Pitta Maíra Galdino da Rocha,



(7) Protective Effects of Oxymatrine on Vascular Endothelial Cells from High-Glucose-Induced Cytotoxicity by Inhibiting the Expression of A2B Receptor.[TOP]

Pubmed ID :29402837
Publication Date : //
Diabetes mellitus (DM) has become an increasingly epidemic metabolic disease. Vascular endothelial cells play a key role in developing the cardiovascular complications of DM. The A2B receptor is expressed in vascular endothelial cells, and may help regulate the function of endothelial cells. The aim of this study was to investigate the protective effects of oxymatrine (OMT) on human umbilical vein endothelial cells (HUVECs) from high glucose-induced cytotoxicity.

Authors : Yi Yun, Shen Yulin, Wu Qin, Rao Jingan, Guan Shu, Rao Shenqiang, Huang Liping, Tan Mengxia, He Lingkun, Liu Lijuan, Li Guodong, Liang Shangdong, Xiong Wei, Gao Yun,



(8) Neuroprotective effect of tanshinone IIA weakens spastic cerebral palsy through inflammation, p38MAPK and VEGF in neonatal rats.[TOP]

Pubmed ID :29257210
Publication Date : //
As one of main active ingredients of salvia miltiorrhizae, which is a traditional Chinese medicine, tanshinone IIA is the basis of its pharmacological activities. In the present study, the effect of tanshinone IIA on weakening spastic cerebral palsy (SCP) in neonatal rats was investigated. Radial arm water maze and holding tests were used to measure the alterations of spastic cerebral palsy, inflammation was measured using an ELISA kit, and western blot analysis was used to analyze the protein expression of p‑p38 mitogen‑activated protein kinase (MAPK) and vascular endothelial growth factor (VEGF). The central mechanisms involved in the mediation or modulation of inflammation, p‑p38 MAPK and VEGF were also investigated. Treatment with tanshinone IIA effectively inhibited spastic cerebral palsy, and the activities of interleukin (IL)‑1β, IL‑6, tumor necrosis factor‑α, monocyte chemoattractant protein 1, cyclooxygenase‑2 and prostaglandin E2 in a neonatal rat model of SCP. Tanshinone IIA effectively suppressed the protein expression of inducible nitric oxide synthase (NOS), phosphorylated (p‑) nuclear factor (NF)‑κB, p‑p38MAPK and VEGF, and activated the phosphorylation of inhibitor of NF‑κB and the protein expression of neuronal NOS in the SCP rat model. These results suggested that the neuroprotective effect of tanshinone IIA weakened SCP through inflammation, p38MAPK and VEGF in the neonatal rats.

Authors : Zhang Wen-Luo, Cao Yue-An, Xia Jing, Tian Li, Yang Lu, Peng Chao-Sheng,



(9) [NLRP3 inflammasome induces pyroptosis in lung tissues of radiation-induced lung injury in mice].[TOP]

Pubmed ID :29089078
Publication Date : //
Objective To establish a radiation-induced lung injury model and investigate the role of caspase-1-dependent programmed cell death (pyroptosis) in the pathogenesis of radiation pneumonitis. Methods BALB/c mice were sacrificed after receiving 5-day 15 Gy X-ray irradiation at chest cavity. The pathological changes of pulmonary tissues were observed by HE staining. The apoptosis of lung tissues cells after irradiation was detected by TUNEL assay. The expressions of γ-H2AX, ki67, NLR family pyrin domain containing 3 (NLRP3), caspase-1, apoptosis-associated speck-like protein containing a CARD (ASC/TMS-1) were detected by Western blot analysis. Real-time quantitative PCR was used to check mRNA levels of interleukin-6 (IL-6), IL-8, tumor necrosis factor α (TNF-α), monocyte chemoattractant protein 1 (MCP-1), NLRP3, caspase-1, IL-1β and IL-18. Immunohistochemical staining was used to determine the expressions of NLRP3, caspase-1 and TMS1 in lung tissues. The activity of caspase-1 was evaluated by caspase-1 assay kit, and the serum levels of IL-1β and IL-18 were detected by ELISA. Results After irradiation, the capillaries of the alveolar wall of the mice were dilated and congested, inflammatory cells infiltrated, the alveolar wall thickened. Positive rate of lung tissue cells was raised in TUNEL staining. The expressions of γ-H2AX and ki67 were elevated, indicating that DNA damage and cell proliferation activity decreased in lung tissues. The mRNA levels of IL-6, IL-8, TNF-α and MCP-1 in lung cells increased; the serum levels of IL-1β and IL-18 increased; the expressions of IL-1β, IL-18, NLRP3, caspase-1 and ASC/TMS-1 in lung tissues were enhanced; and caspase-1 activity increased. Conclusion After irradiation, the pyroptosis caused by the activation of NLRP3 inflammatory body occurred in the lung tissue of mice.

Authors : Han Rong, Wu Dongming, Deng Shihua, Liu Teng, Zhang Ting, Xu Ying,



(10) Urinary neutrophil gelatinase-associated lipocalin and monocyte chemoattractant protein 1 as biomarkers for lupus nephritis in Colombian SLE patients.[TOP]

Pubmed ID :29073812
Publication Date : //
Background Information regarding urinary biomarkers in Mestizo and Afro-Latin-American patients is very limited. We investigated whether levels of urinary neutrophil gelatinase-associated lipocalin (NGAL), and monocyte chemoattractant protein 1 (MCP-1) are good biomarkers to differentiate patients with lupus nephritis among Latin-American systemic lupus erythematosus (SLE) patients. Methods SLE patients meeting the revised American College of Rheumatology classification criteria for SLE were recruited. Urinary levels of NGAL and MCP-1 were measured using a commercial ELISA kit. Serum anti-C1q antibodies were measured by ELISA. SLE activity was measured with the systemic lupus erythematosus disease activity index (SLEDAI). Mann-Whitney tests were used to compare data and Spearman's rank correlations were used to examine associations between continuous variables. In addition, receiver operating characteristic curves were performed. Results One hundred and twenty SLE patients were recruited (87% women) with a median age of 32.8 ± 12.1 years and median disease duration of 7.3 ± 6.9 years. Afro-Latin-Americans had a significantly higher prevalence of lupus nephritis and higher SLEDAI scores than Mestizos. The three biomarkers were significantly higher in patients with lupus nephritis than in patients without lupus nephritis. In addition, urinary NGAL and MCP-1 were significantly higher in patients with active lupus nephritis than in inactive lupus nephritis. Urinary NGAL levels were significantly higher in Afro-Latin-American patients. A receiver operating characteristic curve for urinary biomarkers for lupus nephritis in all SLE patients showed a good level of sensitivity and specificity. Conclusion In our cohort of SLE patients, we found that urinary NGAL and MCP-1 in addition to anti-C1q antibodies were useful biomarkers for the identification of renal involvement and discrimination of active lupus nephritis among patients with renal disease.

Authors : Gómez-Puerta J A, Ortiz-Reyes B, Urrego T, Vanegas-García A L, Muñoz C H, González L A, Cervera R, Vásquez G,