Free Shipping on orders over 50$

British Pound Sterling - GBP Euro - EUR US Dollar - USD (EUR)

Welcom to Gentaur Biotech Products!

ELISA Kit for Insulin Like Growth Factor 1 (IGF1) Organism: Rhesus monkey (Simian)

Be the first to review this product

Availability: In stock


Quick Overview

[#E90050Si] ELISA Kit for Insulin Like Growth Factor 1 (IGF1) Organism: Rhesus monkey (Simian)


E90050Si | ELISA Kit for Insulin Like Growth Factor 1 (IGF1) Organism: Rhesus monkey (Simian), 96T
More informations about ELISA Kit for Insulin Like Growth Factor 1 (IGF1) Organism: Rhesus monkey (Simian) in

Product Tags

Use spaces to separate tags. Use single quotes (') for phrases.

(1) The Tubular Damage Markers: Neutrophil Gelatinase-Associated Lipocalin and Kidney Injury Molecule-1 in Newborns with Intrauterine Growth Restriction.[TOP]

Pubmed ID :30513528
Publication Date : //
Intrauterine growth restriction (IUGR) is a poorly understood complication of pregnancy. It may be associated with various diseases in adulthood, such as hypertension, cardiovascular disease, insulin resistance, and end-stage renal disease.

Authors : Kamianowska Monika, Szczepański Marek, Kulikowska Elżbieta Ewa, Bebko Barbara, Wasilewska Anna,

(2) Early postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomy.[TOP]

Pubmed ID :30474555
Publication Date : //
This study aimed to determine early postoperative changes of serum sphingomyelin (SM) and ceramide (CER) species following laparoscopic sleeve gastrectomy (LSG).

Authors : Özer Hakan, Aslan İbrahim, Oruç Mehmet Tahir, Çöpelci Yaşar, Afşar Ebru, Kaya Sabriye, Aslan Mutay,

(3) Methylation and Acetylation Enhanced the Antidiabetic Activity of Some Selected Flavonoids: In Vitro, Molecular Modelling and Structure Activity Relationship-Based Study.[TOP]

Pubmed ID :30445784
Publication Date : //
Flavonoids have been reported to exert antihyperglycemic effects and have potential to enhance the current therapy options against type 2 diabetes mellitus. However, the structure activity relationships (SAR) studies of flavonoids against this disease have not been thoroughly comprehended. Hence, in the present study, 14 structurally related flavonoids viz. wogonin, techtochrysin, norwogonin, isoscutellarein, hypolaetin, kaempferol, quercetin, methyl ether of wogonin, acetate of wogonin, acetate of norwogonin, 8-hydroxy-7-methoxyflavone, chrysin, (+)-catechin and (-)-epicatechin were taken into account for in vitro antidiabetic evaluation. Cell viability of RIN-5F pancreatic cells and 3T3-L1 pre-adipocyte cells was initially tested, then an insulin secretion assay of RIN-5F as well as adipogenesis and glucose uptake measurements of adipocyte were investigated. Subsequently, protein expressions study through adipokines measurement (leptin, adiponectin, TNF-α, RBP-4) via enzyme-linked immunosorbent assay (ELISA) kit, Western blotting analysis against GLUT4 and C/EBP-α as well as molecular docking against GLUT1 were analyzed. The results from cell culture antidiabetic assays (insulin secretion, adipogenesis, and glucose uptake), protein expressions and molecular docking pointed that the methoxy group at position C-8 is responsible for antidiabetic property of selected flavonoids via glucose uptake mechanism indicated by up regulation of GLUT4 and C/EBP-α expressions. The mechanism could be enhanced by the addition of an acetate group at C-5 and C-7 of the flavone skeleton.

Authors : Ahmed Qamar Uddin, Sarian Murni Nazira, Mat So'ad Siti Zaiton, Latip Jalifah, Arief Ichwan Solachuddin Jauhari, Hussein Nurlaili Najmie, Taher Muhammad, Alhassan Alhassan Muhammad, Hamidon Hanisuhana, Fakurazi Sharida,

(4) Adenosine protects pancreatic beta cells against apoptosis induced by endoplasmic reticulum stress.[TOP]

Pubmed ID :30417434
Publication Date : //
Chronic exposure to high glucose induces endoplasmic reticulum (ER) stress in pancreatic beta cells (PBCs). The previous evidence showed that adenosine modulate PBCs viability and insulin secretion. The aim of this study was to evaluate possible involvement of adenosine in protection of MIN6 β-cells from Tunicamycin (Tu)-induced ER stress. MIN6 cells were cotreated with Tu and different concentrations of adenosine. Cell viability, proliferation, and apoptosis were evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT), 5-bromo-2'-deoxyuridine (Brdu), and colony formation assays. Caspase-12 activity was assayed using the fluorometric method. Thioflavin T (ThT) staining was used for the evaluation of protein aggregation. Insulin secretion was evaluated using specific an ELISA kit. Ca mobilization assayed using Fura2/AM probe. BIP, CHOP, XBP-1, and XBP-1s expression in both messenger RNA (mRNA) and protein levels were evaluated using the reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. Bcl-2, p-eIF2α/eIF2α, and GADD34 levels also determined with Western blot analysis. Adenosine protected MIN6 cells against Tu-induced ER stress in a dose-dependent manner and increased their proliferation. Decreased caspase-12 activity and upregulated Bcl-2 protein may explain antiapoptotic effects of adenosine. ThT staining indicated an attenuated aggregation of misfolded proteins. Adenosine effectively increased insulin secretion in Tu-treated cells. BIP, CHOP, XBP1, and sXBP1 expression were decreased significantly in cotreated cells, indicating alleviation of ER stress. However, adenosine potentiated the expression of GADD34 and decreased p-eIF2α/eIF2α ratio. Adenosine increased cytosolic Ca levels, which may promote adenosine triphosphate (ATP) synthesis in mitochondria, helping ER to preserve protein hemostasis. Taken together, adenosine upregulated Bcl-2 and GADD34 to protect PBCs against Tu-induced apoptosis and increase Insulin secretion.

Authors : Shahrestanaki Mohammad Keyvanloo, Arasi Fatemeh Panahi, Aghaei Mahmoud,

(5) The effect of moderate endurance training on gastrocnemius retinol-binding protein 4 and insulin resistance in streptozotocin-induced diabetic rats.[TOP]

Pubmed ID :30363341
Publication Date : //
Retinol-binding protein 4 (RBP4) is suggested to be involved in the occurrence of insulin resistance. There are contradictory studies about the effects of exercise training on RBP4 levels and insulin resistance. Hence, we designed this study to investigate the impact of moderate endurance training on gastrocnemius RBP4 and insulin resistance in streptozotocin (STZ)-induced diabetic rats.

Authors : Yousefi Mohammad-Reza, TaheriChadorneshin Hossein,

(6) Association of serum total bilirubin and plasma 8-OHdG in HIV/AIDS patients.[TOP]

Pubmed ID :30363337
Publication Date : //
Oxidative stress is a major contributor in the pathogenesis of insulin resistance (IR) and DNA damage in HIV/AIDS patients. Bilirubin has been shown to have antioxidant effects. In this case-control study, 600 subjects were included. We determined serum total bilirubin and IR in all subjects. We measured 8-hydroxy-2-deoxyguanosine with 8-hydroxy-2-deoxyguanosine enzyme-linked immunosorbent assay kit. IR and oxidative DNA damage were significantly higher in HIV-positive patients with second-line antiretroviral therapy (ART) and first-line ART than ART-naive patients. However, average serum total bilirubin was higher in ART-naive patients than the HIV-positive patients with second-line ART and first-line ART. In a logistic regression analysis, serum total bilirubin was negatively associated with the IR [odds ratio (OR): 0.0127, 95% confidence interval (CI): 0.023-0.070,  = 0.0000] and DNA damage (OR: 0.525, 95% CI: 0.351-0.783,  = 0.0016). We found that prevalence of IR and DNA damage was less in ART-naive patients compared with ART first-line and ART second-line HIV-positive patients. Larger studies are warranted to determine the molecular mechanisms involved in the negative association of serum bilirubin and DNA damage in ART naive patients.

Authors : Kolgiri Vaishali, Nagar Vidya, Patil Vinayak,

(7) Effects of casein non-phosphopeptide on the development of rat muscle analyzed using computed tomography scanning technology.[TOP]

Pubmed ID :30352111
Publication Date : //
The effects of casein non-phosphopeptide (CNPP) on the muscle development of healthy rats and selected blood hormones levels were investigated. CT technology and the ELISA kit were employed to detect the cross-sectional area of each muscle group and blood hormone levels, respectively. The cross-sectional area of the trunk and lower limb muscles of resistance exercise group (REG) rats that were administered a high daily dose of CNPP for 50 days increased more significantly than that of the blank group rats, no exercise group (NEG) rats administered with the same daily dose of CNPP, and REG rats administered with the same daily dose of lactalbumin (P < 0.05).The more enhanced development of trunk and lower limb muscles in CNPP-administered REG rats was associated with a higher blood level of insulin, while no clear trends in blood levels of growth hormone and testosterone were observed. The present results have demonstrated that a combination of physical exercise and diet supplementation with CNPP can synergistically improve muscle mass.

Authors : Shi Yan-Guo, Zhang Na, Guo Qing-Qi, Guan Hua-Nan, Ikeda Shinya, Guo Chang-Hui, Piekoszewski Wojciech, Kalenik Tatyana K, Motkina Elwna,

(8) Decreased serum levels of CTRP12/adipolin in patients with coronary artery disease in relation to inflammatory cytokines and insulin resistance.[TOP]

Pubmed ID :30337217
Publication Date : //
Coronary artery disease (CAD) is the leading cause of death worldwide. Atherosclerosis as the main underlying mechanism of CAD is associated with inflammation and adipose tissue dysfunction. C1q/TNF-related protein12 (CTRP12) is a newly discovered adipokine which is a paralog of adiponectin. CTRP12 has anti-inflammatory and insulin sensitizing effects. Circulating levels of this adipokine have been reported to be lower in patients with type 2 diabetes and women with polycystic ovarian syndrome. The present study was undertaken for the first time to evaluate serum levels of CTRP12 in CAD patients and its association with anthropometric and biochemical parameters. Serum levels of CTRP12 were measured using ELISA kit in 188 CAD patients (angiography confirmed) and 70 controls. The serum levels of adiponectin, TNF-α and IL-6 were measured using ELISA kits. Serum levels of CTRP12 were found to be lower in CAD patients (585.48 ± 201.67 pg/mL) than in the controls (814.86 ± 247.85 pg/mL; p < 0.001). CTRP12 also showed an independent association with the risk of CAD (OR [CI] = 0.998 [0.996-0.999]; p = 0.019). Moreover, it showed an inverse correlation with HOMA-IR (r = -0.298; p = 0.012) and TNF-α (r = -0.269; p = 0.023) and a positive correlation with adiponectin (r = 0.344; p = 0.003) in the controls. In CAD patients, CTRP12 was inversely correlated with BMI (r = -0.181, p = 0.013), HOMA-IR (r = -0.199; p = 0.006), TNF-α (r = -0.259; p < 0.001) and IL-6 (r = -320; p < 0.001) and a positive correlation with high density lipoprotein-cholesterol(r = 0.342; p < 0.001) and adiponectin (r = 0.398; p < 0.001). The present study showed for the first time that serum levels of CTRP12 are independently associated with CAD and that CTRP12 is associated with several CAD risk factors. The results suggest a possible link between CTRP12 and pathogenic mechanisms of atherosclerosis, such as inflammation and high density lipoprotein-cholesterol metabolism; however, more study is required in this regard.

Authors : Fadaei Reza, Moradi Nariman, Kazemi Tooba, Chamani Elham, Azdaki Nahid, Moezibady Sayed Ali, Shahmohamadnejad Shiva, Fallah Soudabeh,

(9) A Putative Association of Interleukin-1β Promoter Polymorphisms and IL-1β Levels in Saudi Diabetic Patients.[TOP]

Pubmed ID :30311584
Publication Date : //
Type 2 diabetes mellitus (T2DM) is a well-known endocrine disorder affecting a significant number of individuals across the globe. Risk factors such as inflammation, obesity, high blood glucose level, cardiovascular disease, and genetic alteration are believed to be the reason for T2DM onset. The current study was intended to envisage the possible association between polymorphisms in the interleukin-1β (IL-1β) promoter and IL-1β serum levels in Saudi T2DM patients. Biochemical parameters such as fasting blood glucose (FBS), percentage glycosylated hemoglobin (HbAic), and lipid profile were measured spectrophotometrically. Serum insulin levels were measured using an immunochemistry analyzer. The commercial enzyme-linked immune assay (ELISA) kit was used to estimate the level of IL-1β in the serum samples. The Sanger sequencing method was adopted to determine single-nucleotide polymorphisms (SNPs) in the IL-1β promoter. We observed a significant (P < 0.001) elevation in FBS, Hb1Ac, insulin, and low-density lipoprotein cholesterol in T2DM patients compared with control individuals. Similarly, IL-1β level were found to be increased by 221% in T2DM patients compared with controls. A nonsignificant genotypic association was observed in the IL-1β gene at the -511C/T and -31C/T positions in T2DM patients compared with control individuals. However, a significant association (P < 0.05) was observed at the allelic level. Further studies on larger sample sizes are recommended to establish the exact role of IL-1β polymorphism in the pathogenesis of T2DM and its genetic risk.

Authors : Tabrez Shams, Ashraf Ghulam Md, Hindawi Salwa,

(10) Complement Factor C3 Methylation and mRNA Expression Is Associated to BMI and Insulin Resistance in Obesity.[TOP]

Pubmed ID :30104553
Publication Date : //
Epigenetic marks, and especially DNA methylation, are becoming an important factor in obesity, which could help to explain its etiology and associated comorbidities. Adipose tissue, now considered as an important endocrine organ, produces complement system factors. Complement component 3 () turns out to be an important protein in metabolic disorders, via either inflammation or the subproduct acylation stimulating protein (ASP) which directly stimulates lipid storage. In this study, we analyze DNA methylation in adipose tissue from subjects with a different grade of obesity. Adipose tissue samples were collected from subjects with a different degree of obesity determined by their body mass index (BMI) as: Overweight subjects (BMI ≥ 25 and <30), obese class 1/2 subjects (BMI ≥ 30 and <40) and obese class 3 subjects (BMI ≥ 40). C3 DNA methylation was measured for 7 CpGs by pyrosequencition using the Pyromark technology (Qiagen, Madrid Spain). C3 messenger RNA (mRNA) levels were analyzed by pre-designed Taqman assays (Applied biosystems, Foster City, CA, USA) and ASP/C3a was measured using a ELISA kit. The data were analyzed using the statistic package SPSS. DNA methylation levels were lower in the morbid obese group. Accordingly, methylation correlated negatively with BMI and leptin. However, mRNA levels were more associated with insulin resistance, and positive correlations with insulin, glucose and homeostasis model assessment-estimated insulin resistance (HOMA-IR) existed. ASP correlated negatively with high density lipoprotein (HDL) cholesterol. methylation levels were associated to adiposity variables, such as BMI and leptin, while the mRNA levels were associated to glucose metabolism.

Authors : Castellano-Castillo Daniel, Moreno-Indias Isabel, Fernandez-Garcia Jose Carlos, Clemente-Postigo Mercedes, Castro-Cabezas Manuel, Tinahones Francisco José, Queipo-Ortuño María Isabel, Cardona Fernando,