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Glutathione, Rat anti_; ELISA

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[#OBT7129] Glutathione, Rat anti_; ELISA

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(1) Artepillin C, drupanin, aromadendrin-4'-O-methyl-ether and kaempferide from Brazilian green propolis promote gastroprotective action by diversified mode of action.[TOP]

Pubmed ID :30107246
Publication Date : //
The propolis is extensively used in folk medicine in natura or to prepare pharmaceutical formulations since ancient time to improve health or prevent diseases, among them gastrointestinal disorders. Aiming to contribute in the scientific validation about the popular use of Brazilian Green propolis (BGP) against gastritis and gastric ulcer, this work evaluated the antiulcer potential of isolated compounds from BGP, three prenylated p-coumaric acid derivatives and two flavonoids, respectively named: 3,5 diprenyl-4-hydroxycinnamic acid (artepillin C) (1), 3-prenyl-4-dihydroxycinnamoiloxy cinnamic acid (baccharin) (2), 3-prenyl-4-hydroxycinnamic acid (drupanin) (3), aromadendrin-4'-O-methyl-ether (4) and kaempferide (5).

Authors : Costa Philipe, Almeida Marilia O, Lemos Marivane, Arruda Caroline, Casoti Rosana, Somensi Lincon Bordignon, Boeing Thaise, Mariott Marihá, da Silva Rita de Cássia Melo Vilhena de Andrade Fonseca, Stein Bruna De Paoli, Souza Priscila de, Dos Santos Ana Caroline, Bastos Jairo K, da Silva Luísa Mota, Andrade Sérgio Faloni de,



(2) The protective effect of melatonin on brain ischemia and reperfusion in rats and humans: in vivo assessment and a randomized controlled trial.[TOP]

Pubmed ID :30098076
Publication Date : //
Carotid endarterectomy (CEA) is the treatment of choice for carotid stenosis. Some patients develop ischemia and reperfusion (I/R) injury after CEA. This study was designed to investigate the neuroprotective effects of melatonin on I/R injury in both rats and humans. To this end, 36 male rats were evaluated, and a double-blind randomized controlled trial (RCT) including 60 patients was performed. A rat model of middle cerebral artery occlusion (MCAO) was used to mimic cerebral I/R. After 2 hr of occlusion and 24 hr of reperfusion, blood samples and brain tissues were harvested for further assessments. Compared with the vehicle treatment, melatonin decreased the expression of nuclear factor κ light-chain-enhancer of activated B cells (NF-κB) and S100 calcium-binding protein β (S100β) (p<0.05) and markedly increased the expression of nuclear erythroid 2-related factor 2 (Nrf2), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) (p<0.05). The participants in the RCT took 6 mg/day melatonin orally from 3 days before surgery to 3 days after surgery. Blood samples were drawn at the following times: baseline; pre-anesthesia; carotid reconstruction completion; and 6, 24, and 72 hr after CEA. Compared with the oral placebo treatment, melatonin decreased the expression of NF-κB, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and S100β (p<0.05) and increased the expression of Nrf2, SOD, CAT and GPx (p<0.05) in patients after CEA. Our findings suggested that melatonin could ameliorate brain I/R injury after CEA and that this outcome was essentially due to the antioxidant and anti-inflammatory effects of melatonin. This article is protected by copyright. All rights reserved.

Authors : Zhao Zhewei, Lu Chengran, Li Tianjia, Wang Wenda, Ye Wei, Zeng Rong, Ni Leng, Lai Zhichao, Wang Xuebin, Liu Changwei,



(3) Ameliorative Effect of Silymarin on Scopolamine-induced Dementia in Rats.[TOP]

Pubmed ID :30087724
Publication Date : //
This study aims to elucidate the possible ameliorative effect of silymarin on scopolamine-induced dementia using the object recognition test (ORT) in rats.

Authors : El-Marasy Salma A, Abd-Elsalam Reham M, Ahmed-Farid Omar A,



(4) [Effects of N, N-dimethylformamide on hepatic antioxidant capacity and liver PPARs mRNA levels in rats].[TOP]

Pubmed ID :30081998
Publication Date : //
To investigate the effects of N, N-dimethylformamide(DMF)exposure on liver anti-oxidative capacity and peroxisome proliferator activated receptor(PPAR)αand PPARγin rats.

Authors : Zhang Man, Zheng Min, Wu Zhijun, Wang Lei, Guan Mingyue, Zhao Wenjin, Cheng Juan,



(5) Echinochrome pigment as novel therapeutic agent against experimentally - induced gastric ulcer in rats.[TOP]

Pubmed ID :30081206
Publication Date : //
evaluate the antiulcer healing effect of echinochrome pigment (Ech 5 and 10 mg/kg) extracted from sea urchin (Paracentrotus lividus).

Authors : Sayed Dawlat A, Soliman Amel M, Fahmy Sohair R,



(6) β-catenin contributes to cordycepin-induced MGMT inhibition and reduction of temozolomide resistance in glioma cells by increasing intracellular reactive oxygen species.[TOP]

Pubmed ID :30081068
Publication Date : //
Glioblastoma multiforme (GBM) is one of the most aggressive human tumors, and it has a poor prognosis. Temozolomide (TMZ) is the primary alkylating agent used to treat GBM. Nevertheless, a number of GBM patients are resistant to TMZ. Therefore, there is an urgent need for more effective therapeutic options. Cordycepin (COR) is a natural chemical with anti-tumor effects, although its mechanism of action is poorly understood. Several lines of evidence suggest that O-methylguanine DNA methyltransferase (MGMT) repairs damaged DNA and contributes to drug resistance to TMZ in gliomas. The Wnt/β-catenin pathway regulates MGMT gene expression. However, whether cordycepin inhibits MGMT expression by downregulating the β catenin pathway and augmenting chemosensitivity to TMZ in glioma cells remains unclear. In the present study, we found that cordycepin inhibited the viability of glioma cells and induced apoptosis, cell cycle arrest, overproduction of reactive oxygen species (ROS) and reduction of glutathione (GSH) in vitro. Moreover, cordycepin significantly reduced tumor volume and prolonged median survival of tumor-bearing rats in vivo. We also found that cordycepin inhibited MGMT expression and augmented chemosensitivity to TMZ in glioma cells in vitro and in vivo, accompanied by downregulation of p-GSK-3β and β-catenin. Moreover, overexpression of MGMT reversed the synergistic effect of cordycepin and TMZ. Pharmacological inhibition of GSK-3β with CHIR-99021 or overexpression of β-catenin reversed cordycepin-induced reduction of cell viability, downregulation of β-catenin and MGMT, increase of apoptosis and reduction of TMZ resistance. Furthermore, we found that β-catenin regulated cordycepin-induced overproduction of ROS by decreasing GSH. Inhibition of ROS production with N-acetyl-l-cysteine (NAC) not only rescued the reduction of cell viability but also eliminated β-catenin and MGMT inhibition, prevented glioma cells apoptosis and reversed the synergistic effect of cordycepin and TMZ. Taken together, we demonstrated that β-catenin contributed to cordycepin-induced MGMT inhibition and reduction of TMZ resistance in glioma cells via increasing intracellular ROS. These results indicate that cordycepin may be a novel agent to improve GBM treatment, especially in TMZ-resistant GBM with high MGMT expression.

Authors : Bi Yiming, Li Han, Yi Dazhuang, Bai Yang, Zhong Sheng, Liu Qiaochu, Chen Yong, Zhao Gang,



(7) Molybdenum bupropion combined neurotoxicity in rats.[TOP]

Pubmed ID :30081056
Publication Date : //
Heavy metal toxicity is a common foodborne problem in Egypt, especially in combination. Molybdenum toxicity has been studied as a model of the heavy metal toxicity. Molybdenum could promote toxicity via oxidative-inflammatory mechanisms. Bupropion is a well-known antidepressant that has anti-oxidant mechanisms. It exerts a cytoprotective action against molybdenum induced metal toxicity. The aim of the study is to evaluate the effects of combined bupropion and molybdenum in a toxic animal model. The results showed that the combination of bupropion and high doses of molybdenum was extremely toxic with an evident animal fatality. Bupropion showed a clear anti-oxidant/anti-inflammatory profile detected by the ELISA assay of malondialdehyde (MDA), reduced glutathione, and interleukin -6 (IL-6), and real-time gene expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and tumor necrosis factor-α (TNF-α). The immunohistochemistry of nuclear factor Kappa Beta (NF-κB) showed that bupropion reduced the inflammatory response induced by the molybdenum neurotoxicity. Despite the improved laboratory profile, the animals were extremely intoxicated with recorded fatalities raising the question about other pathways and mechanisms explaining the drug metal interaction. Furthermore, Bupropion even in normal doses was toxic to the animals. Choroid plexus hyperplasia was reported in the histological examination of the animal brain loaded with bupropion, and choroid plexus papilloma was recorded in the combined drug metal group. More wide-scale studies are needed to verify the safety of the current antidepressant medications for the long-term therapy. It is important to focus on drug metal interaction as a possible cause of neuropathology.

Authors : Helaly A M, Mokhtar Naglaa, Firgany Alaa El-Din L, Hazem Noha M, El Morsi E, Ghorab D,



(8) Reno-protective effects of ursodeoxycholic acid against gentamicin-induced nephrotoxicity through modulation of NF-κB, eNOS and caspase-3 expressions.[TOP]

Pubmed ID :30078101
Publication Date : //
Gentamicin (GNT) is a potent aminoglycoside antibiotic widely used to treat life-threatening bacterial infections. We aim to investigate the potential protective effect of ursodeoxycholic acid (UDCA) against GNT-induced nephrotoxicity. In this study, 24 male Wistar rats were used and randomly divided into four groups of six animals each. Control group received 0.5% carboxymethyl cellulose orally for 15 days, GNT group received GNT 100 mg/kg/day i.p. for 8 days, UDCA group received UDCA orally for 15 consecutive days at a dose of 60 mg/kg/day suspended in 0.5% carboxymethyl cellulose and UDCA-pretreated group received UDCA orally for 7 days then co-administered with GNT i.p. for 8 days at the same fore-mentioned doses. Serum levels of kidney function parameters (urea, creatinine, uric acid and albumin) were measured. Renal tissues were used to evaluate oxidative stress markers; malonaldehyde (MDA), reduced glutathione (GSH) and the anti-oxidant enzyme superoxide dismutase (SOD) activities and nuclear factor kappa light-chain enhancer of activated B cells (NF-κB) and kidney injury molecule-1 (KIM-1) mRNA levels. Immunohistochemical expression of endothelial nitric oxide synthase (eNOS) and caspase-3 and histological and ultrastructural examination were performed. Treatment with GNT increased the serum levels of renal function parameters and renal MDA, NF-κB and KIM-1 mRNA levels, while it decreased GSH and SOD activities. Marked immunohistochemical expression of caspase-3 was observed after GNT administration while it decreased eNOS expression. Histological and ultrastructural alterations were also evident in renal corpuscles and tubules. In contrast, pretreatment with UDCA reversed changes caused by GNT administration. These results suggest that UDCA ameliorates GNT-induced kidney injury via inhibition of oxidative stress, inflammation and apoptosis.

Authors : Abd-Elhamid Tarek Hamdy, Elgamal Dalia A, Ali Safaa S, Ali Fares E M, Hassanein Emad H M, El-Shoura Ehab A M, Hemeida Ramadan A M,



(9) Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury.[TOP]

Pubmed ID :30077645
Publication Date : //
Vigna subterranea is widely consumed as a traditional staple food in Nigeria and some West African countries. The ethanolic seed extract of V. subterranea (EEVS) was investigated for its gastroprotective effects on aspirin plus pylorus ligation-induced gastric ulcerated rats using an in vivo assay.

Authors : Balogun Morufu Eyitayo, Besong Elizabeth Enohnyaket, Obimma Jacinta Nkechi, Mbamalu Ogochukwu Sophia, Djobissie Fankou Serges Athanase,



(10) Mechanism mediating the protective effect of diacerein in ischemia-reperfusion-induced testicular injury in rats.[TOP]

Pubmed ID :30076921
Publication Date : //
Torsion of the spermatic cord is a common urologic emergency and can lead to testicular necrosis and infertility. Diacerein (DIA) is interleukin-1b (IL-1b) blocker which has a protective role against myocardial ischemia-reperfusion, however, to-date this role has not been investigated in testicular ischemia- reperfusion (TIR). We aimed to investigate the role and mechanism of action of DIA in induced TIR.

Authors : Abdel-Gaber Seham A, Mohammed Rehab K, Refaie Marwa M M,