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Plant Indole-3-acetic acid ,IAA ELISA Kit, Species Plant, Sample Type serum, plasma

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[#CSB-E09910Pl] Plant Indole-3-acetic acid ,IAA ELISA Kit, Species Plant, Sample Type serum, plasma


CSB-E09910Pl | Plant Indole-3-acetic acid ,IAA ELISA Kit, Species Plant, Sample Type serum, plasma, 96T
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(1) Enhanced Conjugation of Auxin by GH3 Enzymes Leads to Poor Adventitious Rooting in Carnation Stem Cuttings.[TOP]

Pubmed ID :29755501
Publication Date : //
Commercial carnation () cultivars are vegetatively propagated from axillary stem cuttings through adventitious rooting; a process which is affected by complex interactions between nutrient and hormone levels and is strongly genotype-dependent. To deepen our understanding of the regulatory events controlling this process, we performed a comparative study of adventitious root (AR) formation in two carnation cultivars with contrasting rooting performance, "2101-02 MFR" and "2003 R 8", as well as in the reference cultivar "Master". We provided molecular evidence that localized auxin response in the stem cutting base was required for efficient adventitious rooting in this species, which was dynamically established by polar auxin transport from the leaves. In turn, the bad-rooting behavior of the "2003 R 8" cultivar was correlated with enhanced synthesis of indole-3-acetic acid conjugated to aspartic acid by GH3 proteins in the stem cutting base. Treatment of stem cuttings with a competitive inhibitor of GH3 enzyme activity significantly improved rooting of "2003 R 8". Our results allowed us to propose a working model where endogenous auxin homeostasis regulated by GH3 proteins accounts for the cultivar dependency of AR formation in carnation stem cuttings.

Authors : Cano Antonio, Sánchez-García Ana Belén, Albacete Alfonso, González-Bayón Rebeca, Justamante María Salud, Ibáñez Sergio, Acosta Manuel, Pérez-Pérez José Manuel,

(2) Salt tolerance of Glycine max.L induced by endophytic fungus Aspergillus flavus CSH1, via regulating its endogenous hormones and antioxidative system.[TOP]

Pubmed ID :29751251
Publication Date : //
Abiotic stress resistance strategies are powerful approaches to sustainable agriculture because they reduce chemical input and enhance plant productivity. In current study, an endophytic fungus, Aspergillus flavus CHS1 was isolated from Chenopodium album Roots. CHS1 was initially screened for growth promoting activities like siderphore, phosphate solubilization, and the production of indole acetic acid and gibberellins and were further assayed for its ability to promote the growth of mutant Waito-C rice. The results revealed that different plant growth characteristic such as chlorophyll content, root-shoot length, and biomass production were significantly promoted during CHS1 treatment. This growth promotion action was due to the presence of various types of GAs and IAA in the endophyte culture filtrate. Significant up regulation with respect to levels in the control was observed in all endogenous plant GAs, after treatment with CHS1. Furthermore, to evaluate the potential of CHS1 against NaCl stress up to 400 mM, it was tested for its ability to improve soybean plant growth under NaCl stress. In endophyte-soybean interaction, CHS1 association significantly increased plant growth and attenuated the NaCl stress by down regulating ABA and JA synthesis. Similarly, it significantly elevated antioxidant activities of enzymes catalase, polyphenoloxidase, superoxide dismutase and peroxidase as compared to non-inoculated salt stress plants. Thus, CHS1 ameliorated the adverse effect of high NaCl stress and rescued soybean plant growth by regulating the endogenous plant hormones and antioxidative system. We conclude that CHS1 isolate could be exploited to increase salt resistant and yield in crop plants.

Authors : Lubna , Asaf Sajjad, Hamayun Muhammad, Khan Abdul Latif, Waqas Muhammad, Khan Muhammad Aaqil, Jan Rahmatullah, Lee In-Jung, Hussain Anwar,

(3) New insights into auxin metabolism in Bradyrhizobium japonicum.[TOP]

Pubmed ID :29751062
Publication Date : //
Bacterial metabolism of phytohormones includes several processes such as biosynthesis, catabolism, conjugation, hydrolysis and homeostatic regulation. However, only biosynthesis and occasionally catabolism are studied in depth in microorganisms. In this work, we evaluated and reconsidered IAA metabolism in B. japonicum E109, one of the most widely used strains for soybean inoculation around the world. The genomic analysis of the strain showed the presence of several genes responsible for IAA biosynthesis, mainly via indole-3-acetonitrile (IAN), indole-3-acetamide (IAM) and tryptamine (TAM) pathways. However; in vitro experiments showed that IAA is not accumulated in the culture medium in significant amounts. On the contrary, a strong degradation activity was observed after exogenous addition of 0.1 mM of IAA, IBA or NAA to the medium. B. japonicum E109 was not able to grow in culture medium containing IAA as a sole carbon source. In YEM medium, the bacteria degraded IAA and hydrolyzed amino acid auxin conjugates with alanine (IAAla), phenylalanine (IAPhe), and leucine (IAPhe), releasing IAA which was quickly degraded. Finally, the presence of exogenous IAA induced physiological changes in the bacteria such as increased biomass and exopolysaccharide production, as well as infection effectiveness and symbiotic behavior in soybean plants.

Authors : Torres Daniela, Benavidez Iliana, Donadio Florencia, Mongiardini Elias, Rosas Susana, Spaepen Stijn, Vanderleyden Jozef, Pěnčík Aleš, Novák Ondřej, Strnad Miroslav, Frébortová Jitka, Cassán Fabricio,

(4) An Engineered Device for Indoleacetic Acid Production under Quorum Sensing Signals Enables Cupriavidus pinatubonensis JMP134 To Stimulate Plant Growth.[TOP]

Pubmed ID :29746094
Publication Date : //
The environmental effects of chemical fertilizers and pesticides have encouraged the quest for new strategies to increase crop productivity with minimal impacts on the natural medium. Plant growth promoting rhizobacteria (PGPR) can contribute to this endeavor by improving fitness through better nutrition acquisition and stress tolerance. Using the neutral (non PGPR) rhizobacterium Cupriavidus pinatubonensis JMP134 as the host, we engineered a regulatory forward loop that triggered the synthesis of the phytohormone indole-3-acetic acid (IAA) in a manner dependent on quorum sensing (QS) signals. Implementation of the device in JMP134 yielded synthesis of IAA in an autoregulated manner, improving the growth of the roots of inoculated Arabidopsis thaliana. These results not only demonstrated the value of the designed genetic module, but also validated C. pinatubonensis JMP134 as a suitable vehicle for agricultural applications, as it is amenable to genetic manipulations.

Authors : Zúñiga Ana, Fuente Francisco de la, Federici Fernán, Lionne Corinne, Bônnet Jérome, de Lorenzo Victor, González Bernardo,

(5) Genetic diversity and characterization of arsenic-resistant endophytic bacteria isolated from Pteris vittata, an arsenic hyperaccumulator.[TOP]

Pubmed ID :29739310
Publication Date : //
Alleviating arsenic (As) contamination is a high-priority environmental issue. Hyperaccumulator plants may harbor endophytic bacteria able to detoxify As. Therefore, we investigated the distribution, diversity, As (III) resistance levels, and resistance-related functional genes of arsenite-resistant bacterial endophytes in Pteris vittata L. growing in a lead-zinc mining area with different As contamination levels.

Authors : Gu Yunfu, Wang Yingyan, Sun Yihao, Zhao Ke, Xiang Quanju, Yu Xiumei, Zhang Xiaoping, Chen Qiang,

(6) SEUSS and PIF4 Coordinately Regulate Light and Temperature Signaling Pathways to Control Plant Growth.[TOP]

Pubmed ID :29729397
Publication Date : //
Plants continuously monitor environmental conditions (such as light and temperature) and adjust their growth and development accordingly. The transcription factor PHYTOCHROME-INTERACTING FACTOR4 (PIF4) regulates both light and temperature signaling pathways. Here, we identified ENHANCED PHOTOMORPHOGENIC2 (EPP2) as a new repressor of photomorphogenesis in red, far-red, and blue light. Map-based cloning revealed that EPP2 encodes the SEUSS (SEU) transcription regulator. The C-terminus of SEU has transcriptional activation activity and SEU physically interacts with PIF4. Moreover, SEU promotes the expression of many genes, including auxin biosynthetic and responsive genes, and regulates IAA levels in plants. SEU associates with regulatory regions in INDOLE-3-ACETIC ACID INDUCIBLE6 (IAA6) and IAA19 in a PIF4-independent manner, whereas the binding of PIF4 to these genes requires SEU. Furthermore, mutations in SEU affect H3K4me3 methylation at IAA6 and IAA19, and SEU positively regulates warm temperature-mediated hypocotyl growth together with PIF4. Therefore, our results reveal that SEU acts as a central regulator to integrate light and temperature signals to control plant growth by coordinating with PIF4.

Authors : Huai Junling, Zhang Xinyu, Li Jialong, Ma Tingting, Zha Ping, Jing Yanjun, Lin Rongcheng,

(7) Rewiring of auxin signaling under persistent shade.[TOP]

Pubmed ID :29724856
Publication Date : //
Light cues from neighboring vegetation rapidly initiate plant shade-avoidance responses. Despite our detailed knowledge of the early steps of this response, the molecular events under prolonged shade are largely unclear. Here we show that persistent neighbor cues reinforce growth responses in addition to promoting auxin-responsive gene expression in and soybean. However, while the elevation of auxin levels is well established as an early event, in , the response to prolonged shade occurs when auxin levels have declined to the prestimulation values. Remarkably, the sustained low activity of phytochrome B under prolonged shade led to () decreased levels of PHYTOCHROME INTERACTING FACTOR 4 (PIF4) in the cotyledons (the organs that supply auxin) along with increased levels in the vascular tissues of the stem, () elevated expression of the PIF4 targets () and , which in turn reduced the expression of the growth-repressive regulator, () reduced abundance of AUXIN RESPONSE FACTOR 6, () reduced expression of and increased abundance of its targets, the auxin receptors, and () elevated auxin signaling as indicated by molecular markers. Mathematical and genetic analyses support the physiological role of this system-level rearrangement. We propose that prolonged shade rewires the connectivity between light and auxin signaling to sustain shade avoidance without enhanced auxin levels.

Authors : Pucciariello Ornella, Legris Martina, Costigliolo Rojas Cecilia, Iglesias María José, Hernando Carlos Esteban, Dezar Carlos, Vazquez Martín, Yanovsky Marcelo J, Finlayson Scott A, Prat Salomé, Casal Jorge J,

(8) Induction and Generation of Tetraploid Plants of Gapnep.[TOP]

Pubmed ID :29720823
Publication Date : //
Gapnep. is an important medical plant in China. Early researches of were focused on rapid propagation and quality analysis of tissue culture plantlet, and still no research focuses on the plant breeding of and there were no excellent varieties for artificial cultivation of .

Authors : Wei Kun Hua, Xu Jian Ping, Li Lin Xuan, Cai Jin Yuan, Miao Jian Hua, Li Min Hui,

(9) Detection of cytokinins and auxin in plant tissues using histochemistry and immunocytochemistry.[TOP]

Pubmed ID :29701111
Publication Date : //
We report a new method for histochemical localization of cytokinins (CKs) in plant tissues based on bromophenol blue/silver nitrate staining. The method was validated by immunohistochemistry using anti-trans-zeatin riboside antibody. Indole-3-acetic acid (auxin, IAA) was localized by anti-IAA antibody in plant tissues as a proof for IAA histolocalization. We used root sections, because they are major sites of CKs synthesis, and insect galls of Piptadenia gonoacantha that accumulate IAA. Immunostaining confirmed the presence of zeatin and sites of accumulation of IAA indicated by histochemistry. The colors developed by histochemical reactions in free-hand sections of plant tissues were similar to those obtained by thin layer chromatography (TLC), which reinforced the reactive sites of zeatin. The histochemical method for detecting CKs is useful for galls and roots, whereas IAA detection is more efficient for gall tissues. Therefore, galls constitute a useful model for validating histochemical techniques due to their rapid cell cycles and relatively high accumulation of plant hormones.

Authors : Bedetti C S, Jorge N C, Trigueiro Fcg, Bragança G P, Modolo L V, Isaias Rms,

(10) Evaluation of Seaweed Extracts From and spp. as Biostimulants in L. Using a Combination of Chemical, Biochemical and Morphological Approaches.[TOP]

Pubmed ID :29681909
Publication Date : //
Seaweed extracts can be employed as biostimulants during crop cultivation owing to their positive effects on plant performance. Therefore, in this study one extract from (A) and five extracts from (B-F) were assayed on maize ( L.) plants supplied for 2 days with 0.5 mL L of single products to evaluate their capacity to stimulate root growth and morphology, nutrition, and sugars accumulation. Firstly, extracts were chemically characterized via Fourier transform infrared (FT-IR) and FT-Raman spectroscopies, and their content in carbon, nitrogen, phenolic acids and hormones (indole-3-acetic acid, IAA, and Isopentenyladenosine, IPA) was quantified. The auxin like- and gibberellic acid -like activities of all extracts were also determined. FT-IR and FT-Raman spectra provided complementary information depicting distinct spectral pattern for each extract. Bands assigned to alginic and uronic acids were dominant in FT-IR spectra, while those corresponding to polyaromatic rings were evident in FT-Raman spectra. In general, extracts stimulated root growth, nutrition, esterase activity, and sugar content. However, they showed high variation in chemical features, which may explain their different capacity in triggering physiological responses in maize. Among extracts for instance, E was the most efficient in promoting root morphology traits, likely because of its elevate content in IAA (32.43 nM), while F extract was the highest in phenol content (1,933 mg L) and the most successful in improving plant nutrition. On the other hand, C extract was very effective in stimulating root elongation, but did not influence plant nutrition. B and D extracts induced similar positive effects on plants, although they greatly varied in chemical composition. extract (A) differed from extracts, because of its low content in total phenols and the presence of both IAA- and GA-like activity. We conclude that all seaweed extracts acted as biostimulants in maize, but their chemical properties appeared crucial in predicting the physiological response preferentially elicited by individual seaweed extracts.

Authors : Ertani Andrea, Francioso Ornella, Tinti Anna, Schiavon Michela, Pizzeghello Diego, Nardi Serenella,