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Insulin, Guinea pig anti_Bovine; ELISA_RIA

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[#YIAS1011.1] Insulin, Guinea pig anti_Bovine; ELISA_RIA

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YIAS1011.1 | Insulin, Guinea pig anti_Bovine; ELISA_RIA, 20 µl.
More informations about Insulin, Guinea pig anti_Bovine; ELISA_RIA in Antibody-antibodies.com

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(1) A novel sandwich enzyme-linked immunosorbent assay for feline insulin.[TOP]

Pubmed ID :19652495
Publication Date : //
A novel sandwich enzyme-linked immunosorbent assay (ELISA) was established to determine the serum insulin concentrations in domestic cats. By using a solid-phase mouse anti-bovine insulin monoclonal antibody and a peroxidase-conjugated guinea pig anti-rat insulin polyclonal antibody, feline serum insulin concentrations in the range of 0.1 to 3.6 ng/ml could be measured. The intraassay CV and interassay CV were less than 6% and less than 10%, respectively. The present insulin assay will strongly help studies on feline diabetes mellitus.

Authors : Nakaya Mariko, Kito Yuko, Matsuki Naoaki, Shibata Haruki, Touhata Yuki, Tamahara Satoshi, Ono Kenichiro,



(2) Specificity and cross-reactivity of monoclonal antibodies against bovine insulin.[TOP]

Pubmed ID :6084618
Publication Date : //
The positions of antigenic determinants were determined for six monoclonal antibodies against bovine insulin. Three antibodies bound to a determinant around amino acid residue A-10 and the other three to a determinant near the carboxy-terminal of the B-chain. The monoclonal antibodies discriminated between insulin of various species better than did a guinea pig anti-bovine insulin antiserum. One monoclonal antibody discriminated between porcine and human insulin and was used to show that, in principle, it is possible to determine the amount of porcine insulin contained in a mixture of porcine and human insulin.

Authors : Jørgensen P N, Wu C Y, Pedersen P C, Patkar S A, Zeuthen J,



(3) Idiotypes on monoclonal antibodies to bovine insulin. I. Two public idiotypes on anti-bovine insulin hybridomas define idiotypically distinct families of hybridomas.[TOP]

Pubmed ID :6358355
Publication Date : //
We used isoelectric focusing (IEF) to show that individual mice responding to bovine insulin (BI) present complex spectrotypes that are not conserved between individuals. Competition of 125I-BI-reactive bands with cold BI or cold pork insulin (PI), showed that the majority of the antibody response is sensitive to the two amino acid difference between BI and PI. This difference resides in the three amino acid intrachain disulfide-bonded A-loop. To begin dissection of the antibody response to BI, we prepared a panel of 30 monoclonal antibodies against insulin. Anti-idiotypic sera were produced in guinea pigs against seven of these hybridoma proteins (HP), and two, anti-5. 10C6 and anti-5.2B8, were found to define public idiotypes. The level of the 5.10C6 idiotype is controlled by genes linked to the Igh locus. Both the G.P. anti-5.10C6 and G.P. anti-5.2B8 were found to define distinct groups of idiotypically related HP. The 5.10C6 idiotype group contains three closely related members, 5.10C6, 5.5F10, and 5.4C5, which are shown to bear the same L chain by IEF. The 5.2B8 idiotype group has six members that are less closely related, defined by inhibition solid phase RIA. The two most closely related members of this family, 5.2B8 and 5.3C6, possess co-focusing L chains. Within each idiotype group, however, members were found that bind to distinct determinants on insulin. Four of the members of the 5.2B8 idiotype group appear to bind overlapping determinants on insulin that define a topographic region that includes the three amino acid A-loop. Two other HP that have been shown to bind the A-loop do not bear determinants that cross-react with the G.P. anti-5.2B8 antiserum.

Authors : Bender T P, Schroer J, Claflin J L,



(4) SEPARATION OF SKIN-SENSITIZING AND COMPLEMENT-FIXING ANTIBODIES FROM GUINEA PIG ANTI-BOVINE INSULIN SERA.[TOP]

Pubmed ID :14290271
Publication Date : //

Authors : SCHUURS A H, VAN E S, PONDMAN K W,



(5) [Experimental research on insulin antibodies. III. Immunohistology of the endocrine pancreas with guinea pig anti-bovine insulin serum, conjugated with fluorescin isothiocyanate].[TOP]

Pubmed ID :13881607
Publication Date : //

Authors : COSTANZI G, MANCINI U M, ZAMPA G A,