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Guinea Pig Complement C3 ELISA

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[#KT-338] Guinea Pig Complement C3 ELISA

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(1) Hypodermin C improves the survival of kidney allografts.[TOP]

Pubmed ID :30184470
Publication Date : //
Although immunosuppressive therapies have made organ transplantation a common medical procedure worldwide, chronic toxicity is a major issue of long-term treatment. One method to improve such therapies is the application of immunomodulatory agents from parasites, such as Hypoderma lineatum (Diptera: Oestridae). Hypodermin C (HC) is an enzyme secreted by H. lineatum larvae, and our previous study showed that recombinant HC could degrade guinea pig C3 and inhibit the complement pathway in vitro, suggesting potential activity for inhibiting transplant rejection. However, such properties have not been fully demonstrated in vivo. In this study, we investigated the impact of HC on a fully MHC-mismatched, life-sustaining, murine model of kidney allograft rejection using B6 donors and BABL/c (HC transgenic or wild-type) recipients. Kidney grafts were analyzed by histology, immunohistochemistry and western blotting. The results suggested that HC could effectively inhibit kidney allograft rejection. These findings suggest HC is a promising strategy to improve the survival of human implants.

Authors : Hu Ankang, Wang Yan, Yuan Honghua, Wu Lianlian, Zheng Kuiyang,



(2) Protease signaling through protease activated receptor 1 mediate nerve activation by mucosal supernatants from irritable bowel syndrome but not from ulcerative colitis patients.[TOP]

Pubmed ID :29529042
Publication Date : //
The causes of gastrointestinal complaints in irritable bowel syndrome (IBS) remain poorly understood. Altered nerve function has emerged as an important pathogenic factor as IBS mucosal biopsy supernatants consistently activate enteric and sensory neurons. We investigated the neurally active molecular components of such supernatants from patients with IBS and quiescent ulcerative colitis (UC).

Authors : Buhner Sabine, Hahne Hannes, Hartwig Kerstin, Li Qin, Vignali Sheila, Ostertag Daniela, Meng Chen, Hörmannsperger Gabriele, Braak Breg, Pehl Christian, Frieling Thomas, Barbara Giovanni, De Giorgio Roberto, Demir Ihsan Ekin, Ceyhan Güralp Onur, Zeller Florian, Boeckxstaens Guy, Haller Dirk, Kuster Bernhard, Schemann Michael,



(3) Exploring antibody-dependent adaptive immunity against aortic extracellular matrix components in experimental aortic aneurysms.[TOP]

Pubmed ID :29519688
Publication Date : //
Recent evidence suggests that adaptive immunity develops during abdominal aortic aneurysm evolution. Uncertainties remain about the antigens implicated and their role in inducing rupture. Because antigens from the extracellular matrix (ECM) have been suspected, the aim of this experimental study was to characterize the role of adaptive immunity directed against antigens from the aortic ECM.

Authors : Coscas Raphaël, Dupont Sebastien, Mussot Sacha, Louedec Liliane, Etienne Harry, Morvan Marion, Chiocchia Gilles, Massy Ziad, Jacob Marie-Paule, Michel Jean-Baptiste,



(4) Structure characterization of two novel polysaccharides isolated from the spikes of Prunella vulgaris and their anticomplement activities.[TOP]

Pubmed ID :27566209
Publication Date : //
The spikes of Prunella vulgaris have long been used as a traditional Chinese medicine to treat various inflammation-related diseases. The aim of this study was to isolate and characterize homogenous polysaccharides from this herb and to evaluate their anticomplement activity.

Authors : Du Dongsheng, Lu Yan, Cheng Zhihong, Chen Daofeng,



(5) Complement factors C1q, C3 and C5b-9 in the posterior sclera of guinea pigs with negative lens-defocused myopia.[TOP]

Pubmed ID :26309860
Publication Date : //
To investigate the expression of complement factors in the posterior scleral fibroblasts of guinea pigs with negative lens-defocused myopia.

Authors : Gao Ting-Ting, Long Qin, Yang Xue,



(6) Flavonol glycosides and other phenolic compounds from Viola tianshanica and their anti-complement activities.[TOP]

Pubmed ID :26083100
Publication Date : //
Viola tianshanica Maxim. (Violaceae) is a perennial herb distributed in Central Asia, especially in the Xinjiang Uygur Autonomous Region (XUAR) of China. Preliminary study showed that the ethanol extract of the herb exhibited the anti-complement activity against the classical pathway, but the active components responsible for this capacity remain unknown and are yet to be studied.

Authors : Qin Yan, Wen Quan, Cao Jie, Yin Chengle, Chen Daofeng, Cheng Zhihong,



(7) Development of a serological assay to predict antibody bactericidal activity against non-typeable Haemophilus influenzae.[TOP]

Pubmed ID :25927946
Publication Date : //
Non-typeable Haemophilus influenzae (NTHi) is a Gram negative microorganism residing in the human nasopharyngeal mucosa and occasionally causing infections of both middle ear and lower respiratory airways. A broadly protective vaccine against NTHi has been a long-unmet medical need, as the high genetic variability of this bacterium has posed great challenges.

Authors : Ercoli Giuseppe, Baddal Buket, Alessandra Greco, Marchi Sara, Petracca Roberto, Aricò Beatrice, Pizza Mariagrazia, Soriani Marco, Rossi-Paccani Silvia,



(8) Anti-complement sesquiterpenes from Viola yedoensis.[TOP]

Pubmed ID :25562805
Publication Date : //
Two new germacrane sesquiterpenes, yedoensins A (1) and B (2), together with 8 known ones (3-10) were isolated from the herb of Viola yedoensis. The structures of the new compounds were established by extensive spectroscopic means including 1D ((1)H and (13)C) and 2D NMR experiments (HSQC, HMBC, and NOESY) as well as HR-ESI-MS analysis. The absolute configurations of the known sesquiterpenes versicolactone B (3) and madolin W (6) were determined by a modified Mosher's method for the first time. The sesquiterpenes 1-3, and 5-9 exhibited anti-complement activity against the classical pathway (CP) and the alternative pathway (AP) with the CH50 and AP50 values ranging from 0.14 to 0.37mg/mL and 0.32 to 0.54mg/mL, respectively. Preliminary mechanism study using complement-depleted sera showed that yedoensin A (1) and versicolactone B (3) acted on C1q, C3 and C9, while madolin W (6), aristoyunnolin E (7) and madolin Y (9) interacted with C1q, C3, C5 and C9 components in the complement activation cascade.

Authors : Du Dongsheng, Cheng Zhihong, Chen Daofeng,



(9) Immunosuppressive Effect of Hypodermin C on Complement Component 3 In Vitro.[TOP]

Pubmed ID :25424359
Publication Date : //
Hypodermins A (HA), B (HB) and C (HC) are the major proteases secreted by first-instar larvae of Hypoderma lineatum (Diptera: Oestridae). These proteases are involved in the larval migration in the tissue, and prevent the activation of the host immune response. We previously showed that the recombinant HA functions as an immunosuppressive agent which could inhibit the rejection of xenotransplants. In the current study, we cloned the cDNA sequence of HC, which was transfected in Cos7 cells using the pEF1α-IRES-AcGFP expression vector. The Cos7 cells stably expressed HC, and were more resistant to lysis by guinea pig C3 than the control cells. The HC protease degraded the guinea pig C3, and inhibited the complement pathway in vitro. The DNA binding sites of HC were identified using an electrophoretic mobility shift assay. Our findings suggest that the recombinant HC might be useful as an immunosuppressive agent for the inhibition of the xenotransplant rejection.

Authors : Hu An-Kang, Chen Ren-Jin, Zhu Xiao-Rong, Gu Jian-Hong, Liu Zong-Ping,



(10) A novel method for direct measurement of complement convertases activity in human serum.[TOP]

Pubmed ID :24853370
Publication Date : //
Complement convertases are enzymatic complexes that play a central role in sustaining and amplification of the complement cascade. Impairment of complement function leads directly or indirectly to pathological conditions, including higher infection rate, kidney diseases, autoimmune- or neurodegenerative diseases and ischaemia-reperfusion injury. An assay for direct measurement of activity of the convertases in patient sera is not available. Existing assays testing convertase function are based on purified complement components and, thus, convertase formation occurs under non-physiological conditions. We designed a new assay, in which C5 blocking compounds enabled separation of the complement cascade into two phases: the first ending at the stage of C5 convertases and the second ending with membrane attack complex formation. The use of rabbit erythrocytes or antibody-sensitized sheep erythrocytes as the platforms for convertase formation enabled easy readout based on measurement of haemolysis. Thus, properties of patient sera could be studied directly regarding convertase activity and membrane attack complex formation. Another advantage of this assay was the possibility to screen for host factors such as C3 nephritic factor and other anti-complement autoantibodies, or gain-of-function mutations, which prolong the half-life of complement convertases. Herein, we present proof of concept, detailed description and validation of this novel assay.

Authors : Blom A M, Volokhina E B, Fransson V, Strömberg P, Berghard L, Viktorelius M, Mollnes T E, López-Trascasa M, van den Heuvel L P, Goodship T H, Marchbank K J, Okroj M,