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ELISA Kit for Guinea Pig Interferon,Gamma(IFNg)

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[#E90049Gu] ELISA Kit for Guinea Pig Interferon,Gamma(IFNg)


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(1) Serological and Molecular Surveillance of Infectious Bronchitis Virus Infection in Free-Range Chickens and Guinea Fowls in the Ga-East District of Ghana.[TOP]

Pubmed ID :30159336
Publication Date : //
Infectious bronchitis is an economically important disease with worldwide distribution. Information is available on the presence of infectious bronchitis virus in commercial chicken in parts of Ghana but there is no information on free-range poultry and guinea fowls in the country. Possible IBV infections among free-range chickens and guinea fowls in Abokobi and Frafraha communities in the Ga-East district of the Greater Accra Region of Ghana were investigated using serology and PCR. Blood, tracheal, and cloacal swabs were obtained from 219 free-range chickens and guinea fowls with no respiratory symptoms and no history of IBV vaccination. Sera were evaluated for IBV antibodies by ELISA using commercial IBV test kit from IDEXX, Inc., USA. Swab samples were evaluated for S1 glycoprotein gene by one-step RT PCR. All the swab samples tested negative for IBV. 16% of all tested sera were positive for IBV. IBV seroprevalence in guinea fowls was 0%. 21.2% of sera from local chickens were positive for IBV. The seroprevalence of IBV among local chickens from Frafraha was 30% and that of Abokobi was 7.7%. This study shows exposure of local chickens in the study communities to IBV.

Authors : Ayim-Akonor Matilda, Owusu-Ntumy Doreen Dela, Ohene-Asa Hilda Emefa, Oduro-Abrokwa Agyekum, Hammond Patricia, Appenteng Michael, Annan Daniel,

(2) Newcastle disease vaccination in captive-bred wild birds.[TOP]

Pubmed ID :29536316
Publication Date : //
The breeding of wild birds in captivity assumes an increasingly important role in conservation due to the loss of species and their habitats. Providing the environmental and nutritional needs of species kept in captivity is the key for achieving success in such initiatives. Among the flock health practices, we highlight here wild bird vaccination, a scarcely studied subject. This study clinically and serologically evaluates the effect of applying a vaccination protocol against Newcastle disease in three groups of ornamental wild birds. The responses observed in 10 ornamental chickens were compared to those recorded in 12 ring-neck pheasants (Phasianus colchicus), 6 psittacines (2 cockatiels Nymphicus hollandicus, 2 lorikeets Trichoglossus haematodus molucanos, and 2 eastern rosellas Platycercus eximius), and 6 touracos (2 guinea Tauraco persa, 2 white-cheeked Tauraco leucotis, and 2 violet Musophaga violacea). One drop of each live Newcastle HB1 and La Sota vaccines were ocularly instilled on the 1st and 21st experimental days, respectively. On the 112th day, one shot of an inactivated oily Newcastle vaccine was intramuscularly injected. Serum samples were submitted to the Newcastle disease virus antibody Test Kit ELISA-BioChek. Except for the psittacines, other bird species showed a considerable increase in the antibody titers. However, their mean antibody titers differed significantly (P < 0.05) from that recorded in the chickens.

Authors : Gomes C W C, Funkler G, Andretta I, Gonçalves M O, Santos H F, Cruz C E F,

(3) [Sensitivity and specificity of the ELISA Kit for the detection of antidobies to Junin virus].[TOP]

Pubmed ID :26021075
Publication Date : //
The goal of this work was to describe methodological approaches to determination of sensitivity and specificity of the enzyme-linked immunosorbent assay kit (ELISA Kit) for detection of the specific anti-Junin virus (JV) antibody. Comparison of ELISA to plaque reduction neutralization test (PRNT) showed direct relationship between antibody titers in the samples of serum of immunized animals, determined by either PRNT or ELISA methods. The obtained results provided an opportunity to form the panels of positive and negative serum samples to determine the sensitivity and specificity of the ELISA Kit. Sensitivity of the ELISA Kit was at least 98% when studying the samples of serum of immunized guinea pigs and rabbits (determined as positive in PRNT). The sensitivity of the ELISA Kit was at least 68% when studying the samples determined by PNRT as uncertain positive. The specificity was 98%. The specificity of the ELISA Kit was 98%.

Authors : Pirozhkov A P, Timofeev M A, Borisevich I V, Syromiatnikova S I, Shatokhina I V, Pantyukhov V B, Kovalchuk A V, Borisevich S V,

(4) A novel recombinant fibrinogenase of Agkistrodon acutus venom protects against hyperacute rejection via degradation of complements.[TOP]

Pubmed ID :23178656
Publication Date : //
Hyperacute rejection (HAR) is a main barrier in xenotransplantation, which is mediated by the combination of natural antibody to the xenograft and complement activation. Current therapies have focus on the inhibition of complement by development of complement inhibitor and transgenic animal organ. Here, we investigated the effects of rFII, a recombinant fibrinogenase from Agkistrodon acutus venom, on complement and HAR. The degradation effect of rFII on complement was tested by SDS-PAGE, CH50 examination, ELISA Kit and cofocal immunofluorescence microscopy in vitro and in vivo. An ex-vivo rat-to-human perfusion model and a vivo guinea-pig-to-rat heat HAR model were used to determine the protection of rFII against HAR. Our investigation indicated that rFII could significantly degrade human C5, C6, and C9, decrease the activity of complement, and inhibit the MAC deposition on HUVECs membrane in vitro. In addition, serum levels of C1q, C3 and C4 in rat were gradually reduced after infusion of rFII. Importantly, in an ex vivo rat-to-human perfusion model, the survival of rat hearts perfused with human serum treated with rFII (83.36 ± 16.63 min) were significantly longer than that of hearts perfused with fresh human serum(15.94 ± 4.75 min). At the time of 15 minutes after perfusion, functions of hearts added with 50 ug/ml rFII sustained well with heart rates at 283 ± 65.32 beats/minute and LVDP at 13.70 ± 5.45 Kpa, while that of hearts perfused with fresh human serum were severely damaged by HAR with heart rates at 107.77 ± 40.31 beats/minute and LVDP at 1.01 ± 0.83 Kpa. We also found that rFII significantly decreased the levels of C1q, C3 and C4 in human fresh serum perfusate. In a vivo guinea-pig-to-rat heat HAR model, the survival of rat hearts treated with rFII were significantly longer than that of hearts perfused with normal saline; and relieved heart damage by complete activation. Our finding demonstrates the anti-complement property of rFII and its protection against HAR, indicating that rFII might be as a potential therapeutic agent for xenotransplantation.

Authors : Lin Xi, Qi Jie-Zhen, Chen Ming-Hui, Qiu Bi-Tao, Huang Zhen-Hua, Qiu Peng-Xin, Chen Jia-Shu, Yan Guang-Mei,

(5) [Changes of prostaglandin D2,carboxypeptidase A3 and platelet activating factor in guinea pig in anaphylactic shock].[TOP]

Pubmed ID :22812216
Publication Date : //
To detect the changes of leukotriene E4(LTE4), prostaglandin D2(PGD2), carboxypeptidase A3(CPA3) and platelet activating factor (PAF) in guinea pigs died from anaphylactic shock.

Authors : Yang Kai, Guo Xiang-jie, Yan Xue-bin, Gao Cai-rong,

(6) [A guinea pig model of respiratory syncytial virus infection for cough and its neurogenic inflammatory mechanism].[TOP]

Pubmed ID :21914323
Publication Date : //
To establish a guinea pig model of acute and postinfectious cough caused by respiratory syncytial virus (RSV) infection and investigate the role of neurogenic inflammation in its pathogenesis.

Authors : Ye Xin-min, Zhong Nan-shan, Liu Chun-Li, Zhao Jin, Qin Sheng, Chen Ru-chong,

(7) Potent innate immune response to pathogenic leptospira in human whole blood.[TOP]

Pubmed ID :21483834
Publication Date : //
Leptospirosis is caused by pathogenic spirochetes of the genus Leptospira. The bacteria enter the human body via abraded skin or mucous membranes and may disseminate throughout. In general the clinical picture is mild but some patients develop rapidly progressive, severe disease with a high case fatality rate. Not much is known about the innate immune response to leptospires during haematogenous dissemination. Previous work showed that a human THP-1 cell line recognized heat-killed leptospires and leptospiral LPS through TLR2 instead of TLR4. The LPS of virulent leptospires displayed a lower potency to trigger TNF production by THP-1 cells compared to LPS of non-virulent leptospires.

Authors : Goris Marga G A, Wagenaar Jiri F P, Hartskeerl Rudy A, van Gorp Eric C M, Schuller Simone, Monahan Avril M, Nally Jarlath E, van der Poll Tom, van 't Veer Cornelis,

(8) [Cloning and expression of novel swine gene BCL-G(L) in E.coli and preparation of its polyclonal antibody in guinea pigs].[TOP]

Pubmed ID :21138691
Publication Date : //
In order to express a novel gene named as BCL-G(L); of swine in E.coli and prepare its polyclonal antibody.

Authors : Jiang Peng-Fei, Cao Jin-Suo, Liu Chao, Zhao Hai-Ping, Zhang De-Li,

(9) Development and validation of a lateral flow immunoassay using colloidal gold for the identification of serotype-specific foot-and-mouth disease virus O, A and Asia 1.[TOP]

Pubmed ID :20951743
Publication Date : //
A lateral flow immunoassay (LFI) was developed to identify and diagnose foot-and-mouth disease virus (FMDV) serotypes O, A and Asia 1. Antibodies obtained from rabbits and guinea pigs immunized with cell-culture-adapted virus strains (O/CHA/99, A/GS/LX/66, Asia 1/CHN/05) and suckling-mouse adapted virus strains (O/AV99(L), A/AV88(L), Asia 1/YNBS/58) were used as capture antibodies. The diagnostic kit included three immunochromatographic strips of types O, A and Asia 1, and the type-specific results were confirmed by color on the test lines of the three strips. The LFI was evaluated using epithelial and vesicular samples (n=396) prepared from current and historical field samples (provide by the National Foot-and-Mouth Disease Reference Laboratory of China at Lanzhou Veterinary Research Institute). Negative samples (n=95) were collected from healthy animals. The diagnostic sensitivity of the LFI for FMDV serotypes O, A and Asia 1 was 88.3% compared to 89.7% obtained by the reference method of indirect-sandwich ELISA. The sensitivity of the LFI for FMDV type Asia 1 was higher at 92.1% compared to 90.5% for the ELISA. The specificity of the LFI was 97.1% compared with 97.4%.

Authors : Jiang Tao, Liang Zhong, Ren Weiwei, Chen Juan, Zhi Xiaoying, Qi Guangyu, Yang Yamin, Liu Zaixing, Liu Xiangtao, Cai Xuepeng,

(10) Autoimmune markers in lymphoid malignancies.[TOP]

Pubmed ID :18405328
Publication Date : //
Chronic immune stimulation such as Helicobacter pylori (hp) infection, Sjögren's syndrome or coeliac disease may initiate non-Hodgkin lymphoma (NHL). The opposite (appearance of autoimmunity) has also been reported. The aim of this study was to describe the pattern of these immune markers in patients with lymphoid malignancies. Sera from 96 patients with NHL (median age 72, range 38-88, F/M 41/55) were analysed with ELISA to determine the frequency of antibodies against guinea pig (gp) and human recombinant (hr) transglutaminase type 2 (Tg2), and hr factor XIII subunit a* (part of the Tg-family), extractable nuclear antigen (ENA), and hp. As hp antibodies decrease in younger age cohorts a sex- and age-matched control group of 768 persons was used. The control population for transglutaminase antibodies consisted of 59 blood donors, (median 42 years, range 19-65) was analysed with a commercial kit. Gp-Tg2-IgG positivity was documented in 72% and hr-Tg2-IgG positivity in 15% (5% positive controls for both; P < 0.001 and ns, respectively). For IgA 3% had gp-Tg2 and 4% hr-Tg2 (5% in controls: ns for both). Anti-FXIII-IgA positivity was found in 22% (5% in controls; P = 0.03). Unspecific anti-ENA-IgG positivity was found in 24% (P < 0.001), while only 2% had specific ENA autoantibodies. Moreover, 36% were positive for anti-hp-IgG, while controls were positive in 54% (P < 0.001). The frequency of unspecific autoantibodies was increased. No differences could be noted in specific autoantibodies (hr-Tg2-IgA). In contrast, fewer than expected were anti-hp-positive. A defective immune response, similar to that in autoimmune diseases, could contribute to the pathogenesis of lymphoid malignancies.

Authors : Sjöberg K, Roth E B, Gustavsson L, Jönsson C, Simán H, Henriksson G, Stenberg P, Lindblom A, Svensson P,