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Deer Insulin-like growth factor binding protein 3,IGFBP-3 ELISA kit, Species Deer, Sample Type serum, plasma

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[#CSB-E08929De] Deer Insulin-like growth factor binding protein 3,IGFBP-3 ELISA kit, Species Deer, Sample Type serum, plasma

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CSB-E08929De | Deer Insulin-like growth factor binding protein 3,IGFBP-3 ELISA kit, Species Deer, Sample Type serum, plasma, 96T
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(1) The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells.[TOP]

Pubmed ID :29065543
Publication Date : //
Melatonin (MLT) plays an important role in regulating the physiological cycle of seasonal breeding animals. Melatonin receptor I (MT1) is effectively expressed in the cambium layer of deer antler. However, the function and metabolic mechanism of MLT/MT1 signaling in the mesenchymal cells of sika deer remain to be further elucidated. In this work, we detected the effects of MLT/MT1 signaling on mesenchymal cells proliferation and the interaction between MLT/MT1 and IGF1/IGF1-R signaling. The results show that (1) deer antler mesenchymal cells actually express MT1; (2) exogenous melatonin significantly promotes mesenchymal cells proliferation, while MT1 knock-down significantly impairs the positive effects of melatonin; and (3) melatonin significantly enhanced IGF1/IGF1-R signaling, as both the expression of IGF1 and IGF-1R increased, while MT1 knock-down significantly decreased IGF1-R expression and IGF1 synthesis. In summary, these data verified that MLT/MT1 signaling plays a crucial role in antler mesenchymal proliferation, which may be mediated by IGF1/IGF1-R.

Authors : Yang Feifei, He Changjiu, Sun Xuyang, Wang Jing, Luo Can, Liu Guoshi, Yang Liguo, Xiong Jiajun, Huo Lijun,



(2) IGF1 regulates RUNX1 expression via IRS1/2: Implications for antler chondrocyte differentiation.[TOP]

Pubmed ID :28055425
Publication Date : //
Although IGF1 is important for the proliferation and differentiation of chondrocytes, its underlying molecular mechanism is still unknown. Here we addressed the physiologic function of IGF1 in antler cartilage and explored the interplay of IGF1, IRS1/2 and RUNX1 in chondrocyte differentiation. The results showed that IGF1 was highly expressed in antler chondrocytes. Exogenous rIGF1 could increase the proliferation of chondrocytes and cell proportion in the S phase, whereas IGF1R inhibitor PQ401 abrogated the induction by rIGF1. Simultaneously, IGF1 could stimulate the expression of IHH which was a well-known marker for prehypertrophic chondrocytes. Further analysis evidenced that IGF1 regulated the expression of IRS1/2 whose silencing resulted in a rise of IHH mRNA levels, but the regulation was impeded by PQ401. Knockdown of IRS1 or IRS2 with specific siRNA could greatly enhance rIGF1-induced chondrocyte differentiation and reduce the expression of RUNX1. Extraneous rRUNX1 might rescue the effects of IRS1 or IRS2 siRNA on the differentiation. In antler chondrocytes, IGF1 played a role in modulating the expression of RUNX1 through IGF1R. Moreover, attenuation of RUNX1 expression advanced the differentiation elicited by rIGF1, while administration of rRUNX1 to chondrocytes treated with IGF1 siRNA or PQ401 reduced their differentiation. Additionally, siRNA-mediated downregulation of IRS1 or IRS2 in the chondrocytes impaired the interaction between IGF1 and RUNX1. Collectively, IGF1 could promote the proliferation and differentiation of antler chondrocytes. Furthermore, IRS1/2 might act downstream of IGF1 to regulate chondrocyte differentiation through targeting RUNX1.

Authors : Yang Zhan-Qing, Zhang Hong-Liang, Duan Cui-Cui, Geng Shuang, Wang Kai, Yu Hai-Fan, Yue Zhan-Peng, Guo Bin,



(3) Molecular cloning, SNP detection and association analysis of 5' flanking region of the goat IGF1 gene with prolificacy.[TOP]

Pubmed ID :26852275
Publication Date : //
The insulin-like growth factor 1 has an important role in reproduction, foetal development and growth. It regulates the secretion of gonadotrophin releasing hormone, stimulates ovarian function and steroidogenesis. The present study was conducted to characterise the 5' flanking region of goat IGF 1 gene, ascertain ovarian expression of the IGF1 gene, detect SNPs and assess the association with prolificacy in the two indigenous goat breeds of South India viz., low prolific Attappady Black and high prolific Malabari. The 5' flanking region of IGF1 gene was PCR amplified, cloned and sequenced from both breeds. Genotyping was performed in 277 goats from the two genetic groups using the PCR-Single Strand Conformational Polymorphism (SSCP) and the expression of the IGF1 gene in the ovary was analysed by quantitative real time PCR. The 5' flanking region of the IGF1 gene was 601 bp long and located at 450 bp upstream of the start codon. Sequence exhibited 97-99% similarity with that of the sheep, cattle and sika deer IGF1 genes. Three genotypes, PP, PQ and QR were observed at this locus with the frequency of 0.62, 0.30 and 0.08, respectively. Sequencing of the representative PCR products from each genotype revealed two SNPs, g.224A>G and g.227C>T. The population was found to be in Hardy-Weinberg disequilibrium at both loci. Statistical results indicated that these loci were associated with litter size (P ≤ 0.05). However, no significant difference was found in the expression of the IGF1 gene in the ovaries of the two goat breeds. These results suggest the significant influence of the IGF1 gene on prolificacy in goats and identified SNPs would benefit the selection of prolific animals in future breeding programs.

Authors : Thomas Naicy, Venkatachalapathy Thirupathy, Aravindakshan Thazhathuveettil, Raghavan K C,



(4) Study on the Changes in Enzyme and Insulin-like Growth Factor-1 Concentrations in Blood Serum and Growth Characteristics of Velvet Antler during the Antler Growth Period in Sika Deer (Cervus nippon).[TOP]

Pubmed ID :26194228
Publication Date : //
This study was conducted to investigate changes in blood enzyme parameters and to evaluate the relationship between insulin-like growth factor-1 (IGF-1), antler growth and body weight during the antler growth of sika deer (Cervus nippon). Serum enzyme activity and IGF-1 concentrations were measured in blood samples collected from the jugular and femoral veins at regular intervals during the antler growth period. Blood samples were taken in the morning from fasted stags (n = 12) which were healthy and showed no clinical signs of disease. Alfalfa was available ad libitum and concentrates were given at 1% of body weight to all stags. The experimental diet was provided at 9 am with water available at all times. There were no significant differences in alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase during antler growth, but alkaline phosphatase concentrations increased with antler growth progression, and the highest alkaline phosphatase concentration was obtained 55 days after antler casting. Serum IGF-1 concentrations measured from blood samples taken from the jugular vein during antler growth, determined that levels of IGF-1 was associated with body weight and antler growth patterns. Serum IGF-1 concentrations were higher at the antler cutting date than other sampling dates. Antler length increased significantly during antler growth (p<0.001), and there was a similar trend to between right and left beams. Body weight increased with antler growth but was not significant. Consequently it appeared that serum alkaline phosphatase concentration was related to antler growth and both antler growth and body weight were associated positively with IGF-1 concentrations during antler growth.

Authors : Park Jaehyun, Jeon Byongtae, Kang Sungki, Oh Mirae, Kim Myonghwa, Jang Seyoung, Park Pyojam, Kim Sangwoo, Moon Sangho,



(5) [Preparation and Determination of Insulin-like Growth Factor I in Deer Antler, Heart and Blood].[TOP]

Pubmed ID :26080495
Publication Date : //
To optimize the method for preparation of the insulin-like growth factor I (IGF-I ) in deer antler, and to determine the IGF-I in deer antler, heart and blood.

Authors : Chen Fan-bo, Yin Jian-yuan, Liu Jing-yan, Yang Yu-xia, Sun Dan-dan, Liu Ji-hua,



(6) microRNA-18b modulates insulin-like growth factor-1 expression in deer antler cell proliferation by directly targeting its 3' untranslated region.[TOP]

Pubmed ID :25756952
Publication Date : //
Insulin-like growth factor-1 (IGF-1) is a multipromoter gene that has complex biological functions and plays an important role in Chinese sika deer antler cell differentiation and proliferation. microRNAs and their roles in deer antler growth have attracted much attention. In the present study, to investigate the effect of microRNAs on the regulation of IGF-1 during the rapid growth of antlers, miRNA GeneChip analysis and TargetScan Human software were used to screen microRNAs that bind to the 3' untranslated region (3'UTR) of IGF-1. The results indicated that a significantly differential expression of miR-18b was observed in cartilage and mesenchymal of antler tip tissue and the presence of miR-18b-binding sites within the IGF-1 3'UTR. A miR-18b mimic was then transfected into antler cartilage cells to overexpress miR-18b and the expression levels were quantified by real-time PCR. Real-time PCR showed that the expression level of miR-18b in transfected cells was significantly increased compared with the control group (p<0.01). Dual luciferase assays revealed that miR-18b decreased the fluorescence value of the luciferase reporter gene in the group transfected with the wild-type vector of IGF-1 3'UTR. In contrast, the relative luciferase activity in the group transfected with the mutant vector of IGF-1 3'UTR did not change obviously. MTT assays and cell cycle analyses confirmed that overexpression of the miR-18b mimic inhibited the proliferation of cartilage cells. In contrast, transfection of a miR-18b inhibitor increased the cell proliferation rate. Furthermore, Western blot analyses revealed that overexpression of miR-18b mimics downregulated the protein levels of IGF-1, while IGF-1 expression increased after transfection of miR-18b inhibitors. Taken together, our findings show that miR-18b is a potentially novel target in deer antler cell proliferation. miR-18b may modulate IGF-1 expression of sika deer antler.

Authors : Hu Wei, Li Mu, Hu Rui, Li Ting, Meng Xingyu,



(7) A case of central serous chorioretinopathy associated with use of deer antler spray supplement.[TOP]

Pubmed ID :24840532
Publication Date : //
This case report describes central serous chorioretinopathy (CSC) in a healthy man associated with the use of deer antler spray, an athletic supplement purported to contain insulin-like growth factor-1 (IGF-1). The CSC resolved after cessation of the supplement. Currently, there are no reports in the literature linking IGF-1 and CSC. We conclude that agents containing IGF-1, such as deer antler supplements, may be correlated with the development of CSC.

Authors : Wong Sophia S, Morrison-Reyes Joshua A, Smithen Lindsay M,



(8) Identification of microRNA-18a as a novel regulator of the insulin-like growth factor-1 in the proliferation and regeneration of deer antler.[TOP]

Pubmed ID :24563285
Publication Date : //
To investigate the effect of miR-18a on the regulation of the insulin-like growth factor (IGF-1) during growth of antlers in sika deer, miRNA Chip analysis, Target Scan and real-time PCR analysis were used to identify miRNAs that bind to the 3'-UTR of IGF-1. An miR-18a mimic was transfected into antler cartilage cells and the expression levels were quantified by real-time PCR. Dual luciferase assays revealed that miR-18a binds to the 3'-UTR of the IGF-1 gene thus indicating this to be a target gene regulated by miR-18a. MTT assays and cell-cycle analyses confirmed that miR-18a significantly inhibited proliferation of cartilage cells. In contrast, transfection of miR-18a inhibitors increased proliferation. Furthermore, Western blot analysis showed that over-expression of miR-18a down-regulated IGF-1 protein levels while IGF-1 expression was increased after transfection of miR-18a inhibitors. Thus, miR-1 down-regulated IGF-1 expression thus implicating miR-18a as an important regulator of antler proliferation.

Authors : Hu Wei, Li Ting, Wu Lei, Li Mu, Meng Xingyu,



(9) MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene.[TOP]

Pubmed ID :24294913
Publication Date : //
MicroRNAs and their roles in rapid antler growth and regeneration have attracted much attention. In the present study, we examined the effects of microRNAs let-7a and let-7f on antler cell proliferation. We used a luciferase reporter screen to demonstrate that insulin-like growth factor 1 receptor (IGF-1R) can be regulated by let-7a and let-7f. MTT assay confirmed that chondrocyte proliferation was inhibited by let-7a and let-7f mimics. In contrast, transfection of let-7a and let-7f inhibitors increased chondrocyte proliferation, indicating that inhibitors can competitively bind to endogenous miRNA, reducing the inhibitory effect of miRNA. Moreover, western blotting analysis further identified that let-7a and let-7f mimics suppressed IGF-1R expression, and that let-7a and let-7f inhibitors increased the expression level of IGF-1R. Taken together, our study demonstrates the important roles of let-7a and let-7f in antler proliferation and its potential application in antler development. let-7a and let-7f may represent novel regulatory factors of IGF-1R expression in deer antler.

Authors : Hu Wei, Li Ting, Hu Rui, Wu Lei, Li Mu, Meng Xingyu,



(10) Comparison of chemical compositions and osteoprotective effects of different sections of velvet antler.[TOP]

Pubmed ID :24212078
Publication Date : //
Velvet antlers (VA) have been claimed for centuries to have numerous medical benefits including strengthen bones. To investigate and compare the anti-osteoporotic activities from different sections of VA.

Authors : Tseng Sung-Hui, Sung Chun-Hsien, Chen Lih-Geeng, Lai Ying-Jang, Chang Wei-Shun, Sung Hsin-Ching, Wang Ching-Chiung,