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Mannan Binding Lectin (MBL), Clone 182_01, Mab anti_Chicken, ELISA_WB_IH

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[#HYB182-01] Mannan Binding Lectin (MBL), Clone 182_01, Mab anti_Chicken, ELISA_WB_IH

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HYB182-01 | Mannan Binding Lectin (MBL), Clone 182_01, Mab anti_Chicken, ELISA_WB_IH, 200 µg.
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(1) The Role of Complement Activating Collectins and Associated Serine Proteases in Patients With Hematological Malignancies, Receiving High-Dose Chemotherapy, and Autologous Hematopoietic Stem Cell Transplantations (Auto-HSCT).[TOP]

Pubmed ID :30294330
Publication Date : //
We conducted a prospective study of 312 patients (194 with multiple myeloma, 118 with lymphomas) receiving high-dose conditioning chemotherapy and autologous hematopoietic stem cell transplantation (auto-HSCT). Polymorphisms of and genes were investigated and serial measurements of serum concentrations of mannose-binding lectin (MBL), CL-LK collectin and MASP-2 as well as activities of MBL-MASP-1 and MBL-MASP-2 complex were made. Serum samples were taken before conditioning chemotherapy, before HSCT and once weekly after (totally 4-5 samples); in minority of subjects also 1 and/or 3 months post transplantation. The results were compared with data from 267 healthy controls and analyzed in relation to clinical data to explore possible associations with cancer and with chemotherapy-induced medical complications. We found a higher frequency of MBL deficiency-associated genotypes (LXA/O or O/O) among multiple myeloma patients compared with controls. It was however not associated with hospital infections or post-HSCT recovery of leukocytes, but seemed to be associated with the most severe infections during follow-up. Paradoxically, high MBL serum levels were a risk factor for prolonged fever and some infections. The first possible association of gene 3'-untranslated region polymorphism with cancer (lymphoma) in Caucasians was noted. Heterozygosity for gene +359 A>G mutation was relatively frequent in lymphoma patients who experienced bacteremia during hospital stay. The median concentration of CL-LK was higher in myeloma patients compared with healthy subjects. Chemotherapy induced marked increases in serum MBL and MASP-2 concentrations, prolonged for several weeks and relatively slighter decline in CL-LK level within 1 week. Conflicting findings on the influence of MBL on infections following chemotherapy of myeloma and lymphoma have been reported. Here we found no evidence for an association between MBL deficiency and infection during the short period of neutropenia following conditioning treatment before HSCT. However, we noted a possible protective effect of MBL during follow-up, and suspected that to be fully effective when able to act in combination with phagocytic cells after their recovery.

Authors : Świerzko Anna S, Michalski Mateusz, Sokołowska Anna, Nowicki Mateusz, Eppa Łukasz, Szala-Poździej Agnieszka, Mitrus Iwona, Szmigielska-Kapłon Anna, Sobczyk-Kruszelnicka Małgorzata, Michalak Katarzyna, Gołos Aleksandra, Wierzbowska Agnieszka, Giebel Sebastian, Jamroziak Krzysztof, Kowalski Marek L, Brzezińska Olga, Thiel Steffen, Jensenius Jens C, Kasperkiewicz Katarzyna, Cedzyński Maciej,



(2) Antimicrobial peptide LL-37 and recombinant human mannose-binding lectin express distinct age- and pathogen-specific antimicrobial activity in human newborn cord blood .[TOP]

Pubmed ID :30271580
Publication Date : //
There is a need to prevent and treat infection in newborns. One approach is administration of antimicrobial proteins and peptides (APPs) such as LL-37, a membrane-active cathelicidin antimicrobial peptide, and mannose-binding lectin (MBL), a pattern-recognition protein that binds to microbial surface polysaccharides resulting in opsonization and complement activation. Low plasma/serum levels of LL-37 and of MBL have been correlated with infection and exogenous administration of these agents may enhance host defense. The antimicrobial activity of LL-37 (15 µg/ml) or rMBL (0.5, 2 and 10 µg/ml) was tested in hirudin-anticoagulated preterm and term human cord blood (N = 12-14) against (SA) USA 300 (2x10 CFU/ml), (SE) 1457 (2x10 CFU/ml) and (CA) SC5314 (1x10 CFU/ml). After incubation (1, 45, or 180 min), CFUs were enumerated by plating blood onto agar plates. Supernatants were collected for measurement of MBL via ELISA. Preterm cord blood demonstrated impaired endogenous killing capacity against SA and SE compared to term blood. Addition of LL-37 strongly enhanced antimicrobial/antifungal activity vs SA, SE and CA in term blood and SE and CA in preterm blood. By contrast, rMBL showed modest fungistatic activity vs CA in a sub-analysis of term newborns with high basal MBL levels. Baseline MBL levels varied within preterm and term cohorts with no correlation to gestational age. In summary, exogenous LL-37 demonstrated significant antimicrobial activity against SA, SE and CA in term and SE and CA in preterm human blood tested . rMBL demonstrated modest antifungal activity in term cord blood of individuals with high baseline MBL levels. To the extent that our results predict the effects of APPs , development of APPs for prevention and treatment of infection should take into account host age as well as the target pathogen.

Authors : Scheid Annette, Li Ning, Jeffers Carleen, Borriello Francesco, Joshi Sweta, Ozonoff Al, Pettengill Matthew, Levy Ofer,



(3) High levels of urinary complement proteins are associated with chronic renal damage and proximal tubule dysfunction in IgA nephropathy.[TOP]

Pubmed ID :30141239
Publication Date : //
Complement activation is involved in the pathogenesis and progression of IgA nephropathy (IgAN); however, the clinical implication of abnormal complement protein levels in serum and urine is not clear. To address this we analysed the correlation between disease activity and complement proteins in serum and urine from IgAN patients, and compared to patients with other types of chronic kidney disease (CKD) as well as healthy controls.

Authors : Wen Lu, Zhao Zhanzheng, Wang Zheng, Xiao Jing, Birn Henrik, Gregersen Jon Waarst,



(4) Basic inflammatory indices and chosen neutrophil receptors expression in chronic haemodialysed patients.[TOP]

Pubmed ID :30135629
Publication Date : //
End stage renal disease (ESRD) patients on chronic haemodialysis (HD) are immuno-compromised and prone to infection. Toll-like receptors (TLRs) play a role as both primary sensors of pathogen invasion and activators of inflammatory reaction. To test if the immune impairment in HD patients is connected with the defective expression of the neutrophil TLRs, we aimed to examine their expression and chosen inflammatory indices.

Authors : Baj Zbigniew, Zbróg Zbigniew, Szuflet Adam, Mańka Sylwia, Bartnicki Piotr, Majewska Ewa,



(5) Interaction of mannose binding lectin and other pattern recognition receptors in human corneal epithelial cells during Aspergillus fumigatus infection.[TOP]

Pubmed ID :30096599
Publication Date : //
Mannose binding lectin (MBL), a member of C-type lectin superfamily, plays a significant role in the innate immune system as one of the Pattern-Recognition Receptors (PRRs). This study investigated the relationship between MBL and other PRRs including Dectin-1and TLR2, and detected the reaction of MBL towards the expression of cytokines in human corneal epithelial cells (HCECs) and murine corneas upon Aspergillus fumigatus (A. fumigatus) hyphae infection. We found that MBL was significantly up-regulated in C57BL/6 corneas infected with A. fumigatus and HCECs incubated with A. fumigatus hyphae. Moreover, both of mRNA and protein levels of Dectin-1, TLR2, IL-1β and TNF-α were increased dramatically to peak point with the pretreatment of exogenous MBL, while decreased significantly with the pretreatment of MBL monoclonal antibody in HCECs. Pretreatment with laminarin and TLR2 neutralizing antibody decreased both of mRNA and protein levels of MBL and proinflammatory mediators (IL-1β and TNF-α) in A. fumigatus hyphae infected HCECs. These data demonstrate that A. fumigatus introduce the expression of MBL in human corneas, C57BL/6 corneas and HCECs. MBL, Dectin-1 and TLR2 interact with each other upon the treatment of A. fumigatus, and MBL contributes to the innate immune responses by regulating proinflammatory mediators, such as IL-1β and TNF-α.

Authors : Peng Xudong, Zhao Guiqiu, Lin Jing, Li Cui,



(6) Molecular profile of mannan-binding lectin in hepatitis C patients with MBL gene polymorphisms by a modified mannan-coated nitrocellulose assay.[TOP]

Pubmed ID :30056939
Publication Date : //
The aim of this study was to develop an assay to analyze the serum profile of Mannose-binding lectin (MBL) through a simple and "in-house" method (called "dot-N-man"). Furthermore, the study attempted to associate molecular masses of MBL to the profile of MBL gene polymorphisms in patients with hepatitis C. Heterogeneity in molecular masses of MBL is due to the impairment of oligomers formation, which is linked to genetic polymorphisms in the MBL gene. Individuals with AA genotype (wild-type) produce high-molecular-mass proteins, whereas AO and OO individuals produce intermediate and low-molecular-mass proteins, respectively. Sera of thirty patients carrying the hepatitis C virus (HCV) were investigated using MBL binding assay with mannan-coated nitrocellulose (dot-N-man). Purified MBL was evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Dot-N-Man assay yielded MBL with molecular masses ranging between 55 and 320 kDa, comparable to low and high molecular mass forms of MBL. Nonreducing SDS-PAGE showed high molecular mass bands in all AA individuals while bands of 270 and 205 kDa were observed in sera for a number of patients with AO and OO genotypes, respectively. Immunoblotting confirmed the MBL samples obtained from the dot-N-man. These results provide new insights to understand the MBL molecular forms profile in patients infected with HCV- which could be useful in future investigations on the influence of the MBL structure/genotype on both the progression of infection and the response to hepatitis C therapy.

Authors : Albuquerque Diego A P, Cavalcanti Igor T, Vasconcelos Luydson R S, Montenegro Francisco, Pereira Leila M M B, Cavalcanti Maria S M, Moura Patrícia, Júnior Luiz B C, de Almeida Sinara Mônica Vitalino, Beltrão Eduardo I C,



(7) Associations between complement pathways activity, mannose-binding lectin, and odds of unprovoked venous thromboembolism.[TOP]

Pubmed ID :30015228
Publication Date : //
Deep vein thrombosis (DVT) originates in the valvular sinuses of large veins in a local milieu characterized by stasis and severe hypoxia. This may induce complement- and coagulation activation, which potentially increases the risk of venous thromboembolism (VTE). The aim of the present study was to investigate whether the activity of the complement pathways, the level of mannose-binding lectin (MBL) and tissue-factor (TF) induced thrombin generation were associated with risk of unprovoked VTE.

Authors : Høiland Ina Isabella, Liang Robin Amanda, Hindberg Kristian, Latysheva Nadezhda, Brekke Ole-Lars, Mollnes Tom Eirik, Hansen John-Bjarne,



(8) Interaction of Mannose-Binding Lectin With Lipopolysaccharide Outer Core Region and Its Biological Consequences.[TOP]

Pubmed ID :30008719
Publication Date : //
Lipopolysaccharide (LPS, endotoxin), the main surface antigen and virulence factor of Gram-negative bacteria, is composed of lipid A, core oligosaccharide, and O-specific polysaccharide (O-PS) regions. Each LPS region is capable of complement activation. We have demonstrated that LPS of , an opportunistic human pathogen, reacts strongly with human and murine mannose-binding lectins (MBLs). Moreover, MBL-LPS interactions were detected for the majority of other Gram-negative species investigated. was used as a model pathogen to investigate the biological consequences of these interactions. The core oligosaccharide region of LPS was identified as the main target for human and murine MBL, especially l--d--heptose (Hep) and -acetyl-d-glucosamine (GlcNAc) residues within the outer core region. MBL-binding motifs of LPS are accessible to MBL on the surface of bacterial cells and LPS aggregates. Generally, the accessibility of outer core structures for interaction with MBL is highest during the lag phase of bacterial growth. The LPS core oligosaccharide-MBL interactions led to complement activation and also induced an anaphylactoid shock in mice. Unlike O3 LPS, robust lectin pathway activation of LPS was mainly the result of outer core recognition by MBL; involvement of the O-PS is not necessary for anaphylactoid shock induction. Our results contribute to a better understanding of MBL-LPS interaction and may support development of therapeutic strategies against sepsis based on complement inhibition.

Authors : Man-Kupisinska Aleksandra, Swierzko Anna S, Maciejewska Anna, Hoc Monika, Rozalski Antoni, Siwinska Malgorzata, Lugowski Czeslaw, Cedzynski Maciej, Lukasiewicz Jolanta,



(9) Substitution of Mannan-Binding Lectin (MBL)-Deficient Serum With Recombinant MBL Results in the Formation of New MBL/MBL-Associated Serine Protease Complexes.[TOP]

Pubmed ID :29997613
Publication Date : //
The lectin pathway (LP) of complement activation depends on the activation of the MBL-associated serine proteases (MASPs) circulating in complex with mannan-binding lectin (MBL). MBL deficiency is the most common complement deficiency and has been associated with several pathological conditions. As we had previously shown, plasma-derived MBL (pdMBL) contains pre-activated MASPs that upon pdMBL substitution results in restoration of MBL concentrations but no LP functionality due to immediate inactivation of pdMBL-MASP complexes upon infusion. In this study, we analyzed MBL-sufficient and -deficient serum by size-exclusion chromatography for complexes of LP activation. In both sera, we identified non-bound free forms of MASP-2 and to lesser extent MASP-1/3. After addition of recombinant MBL (rMBL) to MBL-deficient serum, these free MASPs were much less abundantly present, which is highly suggestive for the formation of high-molecular complexes that could still become activated upon subsequent ligand binding as shown by a restoration of C4-deposition of MBL-deficient serum. Ficolin (FCN)-associated MASPs have been described to redistribute to ligand-bound MBL, hereby forming new MBL/MASP complexes. However, reconstitution of MBL-deficient serum with rMBL did not change the relative size of the FCN molecules suggestive for a limited redistribution in fluid phase of already formed complexes. Our findings demonstrate that rMBL can associate with free non-bound MASPs in fluid phase while preserving full restoration of LP functionality. In contrast to pdMBL products containing pre-activated MASPs which become inactivated almost immediately, these current data provide a rationale for substitution studies using rMBL instead.

Authors : Keizer Mischa P, Kamp Angela, van Mierlo Gerard, Kuijpers Taco W, Wouters Diana,



(10) Cardiovascular risk and mannose binding lectin in patients with rheumatoid arthritis from southern Brazil.[TOP]

Pubmed ID :29992184
Publication Date : //
Mannose binding lectin (MBL) appears to be involved in susceptibility to rheumatoid arthritis (RA), in the inflammatory process and in the genesis of atherosclerotic disease.

Authors : Kahlow Barbara S, Nisihara Renato, Petisco Roberta, Utiyama Shirley R R, Messias-Reason Iara J, Goeldner Isabela, Skare Thelma L,