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MRP 8, Calgranulin A, s100A8, ELISA Kit (includes Catch_Detect Ab's, Std., NO plate)

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[#BMAS1007] MRP 8, Calgranulin A, s100A8, ELISA Kit (includes Catch_Detect Ab's, Std., NO plate)


BMAS1007 | MRP 8, Calgranulin A, s100A8, ELISA Kit (includes Catch_Detect Ab's, Std., NO plate), kit
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(1) Study of serial serum myeloid-related protein 8/14 as a sensitive biomarker in Takayasu arteritis: a single centre study.[TOP]

Pubmed ID :29196802
Publication Date : //
The aim of the study was to explore utility of serial serum myeloid-related protein 8/14 (MRP8/14) as a biomarker of clinical disease activity and angiographic progression in Takayasu arteritis (TA). Serum MRP8/14 levels were assayed by commercial ELISA for 85 TA patients and 24 healthy controls at baseline, and for 56 and 21 TA patients during follow-up visits R1 and R2, respectively. Disease was categorised as active, indeterminate and stable according to Indian Takayasu Arteritis score (ITAS 2010), ITAS-A(CRP) and angiography. Patients were divided into responders and non-responders/relapsers based on treatment response. Non-parametric tests were used for inter-group comparisons at baseline and during follow-up time points. Generalised Estimating Equation was used to study association between changes in serial MRP8/14 levels and disease activity. At baseline, median MRP8/14 levels were higher in patients with TA than healthy controls [7353 (4524 to11283) vs 4896 (3194 to 8474.5) ng/ml, p = 0.011]. Patients with active disease had higher levels [8552 (5463-12488)] than stable disease [5292.5 (3140.5-7310)], p = 0.002, and healthy controls [4896 (3194-8474.5)], p = 0.001. Changes in serial MRP8/14 level were associated with changes in disease activity, independent of steroid dose, p = 0.000. At R1, MRP 8/14 levels were lower than baseline in responders (n = 38) [9146.0 (6296.8-13693.8) vs 6501 (4314.8-8304.5), p = 0.004], but did not change in non-responders/relapsers (n = 14) [6693.5(4210.8-10516.3) vs 7755.0(5342-10741.0), p = 0.42]. Similar trend was observed at R2. MRP8/14 levels increased during follow-up in 66% and 26.3% of angiographic progressors and non-progressors, respectively. MRP8/14 in TA may act as a novel biomarker with prognostic implications.

Authors : Goel Ruchika, Nair Aswin, Kabeerdoss Jayakanthan, Mohan Hindhumathi, Jeyaseelan Visalakshi, Joseph George, Danda Debashish,

(2) Myeloid-related protein-8/14 in acute coronary syndrome.[TOP]

Pubmed ID :28986057
Publication Date : //
The alarmin family member myeloid-related protein (MRP)-14 (S100A9), which has been identified by platelet transcriptional profiling as an acute myocardial infarction gene, regulates vascular inflammation and thrombosis. Elevated plasma levels of MRP-8/14 (S100A8/A9) heterodimer predict first and recurrent cardiovascular events. The aim of this study was to elucidate pathophysiological roles of MRP-8/14 in acute coronary syndrome (ACS).

Authors : Sakuma Masashi, Tanaka Atsushi, Kotooka Norihiko, Hikichi Yutaka, Toyoda Shigeru, Abe Shichiro, Taguchi Isao, Node Koichi, Simon Daniel I, Inoue Teruo,

(3) Nickel Sequestration by the Host-Defense Protein Human Calprotectin.[TOP]

Pubmed ID :28573847
Publication Date : //
The human innate immune protein calprotectin (CP, S100A8/S100A9 oligomer, calgranulin A/calgranulin B oligomer, MRP-8/MRP-14 oligomer) chelates a number of first-row transition metals, including Mn(II), Fe(II), and Zn(II), and can withhold these essential nutrients from microbes. Here we elucidate the Ni(II) coordination chemistry of human CP. We present a 2.6-Å crystal structure of Ni(II)- and Ca(II)-bound CP, which reveals that CP binds Ni(II) ions at both its transition-metal-binding sites: the HisAsp motif (site 1) and the His motif (site 2). Further biochemical studies establish that coordination of Ni(II) at the hexahistidine site is thermodynamically preferred over Zn(II). We also demonstrate that CP can sequester Ni(II) from two human pathogens, Staphylococcus aureus and Klebsiella pneumoniae, that utilize this metal nutrient during infection, and inhibit the activity of the Ni(II)-dependent enzyme urease in bacterial cultures. In total, our findings expand the biological coordination chemistry of Ni(II)-chelating proteins in nature and provide a foundation for evaluating putative roles of CP in Ni(II) homeostasis at the host-microbe interface and beyond.

Authors : Nakashige Toshiki G, Zygiel Emily M, Drennan Catherine L, Nolan Elizabeth M,

(4) Elevated levels of serum MRP8/14 in ankylosing spondylitis: associated with peripheral arthritis and active disease.[TOP]

Pubmed ID :27738838
Publication Date : //
Monocytes of patients with ankylosing spondylitis (AS) have toll-like receptor 4 (TLR4) overexpression on their monocytes. Myeloid-related protein (MRP) 8/14 protein complexes are calcium-binding proteins, which act as endogenous ligands to TLR4. Thus, we studied the levels of MRP8/14 in adult AS patients. MRP8/14 levels were assessed in 99 adult AS patients satisfying Assessments in Ankylosing Spondylitis International Society 2010 criteria and 71 healthy controls by ELISA. Patient disease parameters like patient and physician global assessment, Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), swollen and tender joint count, entheseal count by Maastricht enthesitis index, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) were also recorded. Levels were reassessed in 23 patients after 2-5 months of treatment with NSAIDs. All values are in median (IQR). The serum MRP8/14 levels in patients [34.1 (17.94-264.58) μg/ml] were significantly higher than in healthy controls [4.94 (IQR 3.01-8.32) μg/ml (p < 0.0001)]. Patients with peripheral arthritis (n = 50) had higher levels than those with pure axial disease (n = 49) [40.63 (IQR 28.41-73.15) μg/ml vs. 23.72 (11.04-61.55) μg/ml; p = 0.012]. Levels of MRP8/14 correlated with AS Disease Activity Score (DAS)-CRP (r = 0.23, 95%CI = 0.038-0.422, p = 0.02) and CRP (r = 0.28, 95%CI = 0.081-0.45, p = 0.01), and the correlation was better in early disease [≤5 years disease duration; r = 0.40, p = 0.007 and r = 0.57, p = <0.0001, respectively]. Baseline levels were higher in treatment responders than in non-responders [51.17 vs. 32.22 μg/ml; p = 0.02]. Change in MRP8/14 levels correlated with change in BASDAI and ASDAS-CRP (r = 0.489, p = 0.018 and r = 0·498, p = 0.016, respectively). MRP8/14 levels may be used as a biomarker for activity, peripheral arthritis, and response to therapy.

Authors : Gupta Latika, Bhattacharya Shruti, Agarwal Vikas, Aggarwal Amita,

(5) Low pretreatment levels of myeloid-related protein-8/14 and C-reactive protein predict poor adherence to treatment with tumor necrosis factor inhibitors in juvenile idiopathic arthritis.[TOP]

Pubmed ID :27562034
Publication Date : //
Two thirds of patients with juvenile idiopathic arthritis (JIA) treated with tumor necrosis factor (TNF)-alpha inhibitors respond initially, but only about one third of patients achieve clinical remission at follow-up. We evaluated the 1-year response and long-term treatment adherence to TNF inhibitor treatment in JIA patients naive to biologics and investigated if baseline myeloid-related protein (MRP)-8/14 and C-reactive protein (CRP) were predictive of treatment response. One hundred fifty-two patients were included in a unicenter observational, prospective study from 2002 to 2015, excluding patients with systemic-onset JIA. One-year treatment response was evaluated by American College of Rheumatology-pediatric (ACR-ped) and by the number of patients achieving inactive disease (ID). Medical charts were reviewed for reasons of treatment withdrawal. After one year of treatment ACR-ped 30, 50, 70, and 90 were achieved by 61, 55, 38, and 10 % of the patients, and 23 % achieved a status of ID. Treatment adherence: 51 % withdrew from treatment due to lack of clinical effect, while 32 % continued treatment or withdrew due to disease remission. Increased MRP-8/14 concentrations at treatment initiation was associated with ID after 1 year (OR 1.55, CI 1.06-2.25, p = 0.02). Treatment withdrawal due to lack of effect was associated with low baseline levels of both MRP-8/14 (685 vs. 1235 ng/ml, p < 0.001) and CRP (0.75 vs. 2.73 mg/l, p < 0.001), verified by multivariable logistic regression analysis (OR 0.51, CI 0.34-0.77/OR 0.63, CI 0.48-0.83). In conclusion, an association was found between ID after 1 year of treatment and increased baseline levels of MRP-8/14. Furthermore, low baseline MRP-8/14 and CRP concentrations were associated with treatment withdrawal due to lack of clinical effect.

Authors : Alberdi-Saugstrup Mikel, Nielsen Susan, Mathiessen Pernille, Nielsen Claus Henrik, Müller Klaus,

(6) Myeloid-related Protein 8/14 Levels in Rheumatoid Arthritis: Marker of Disease Activity and Response to Methotrexate.[TOP]

Pubmed ID :26834220
Publication Date : //
Myeloid-related proteins (MRP) 8/14 belong to a family of calcium-binding proteins produced by myeloid cells. Baseline serum levels of MRP8/14 have been shown to predict response to biologicals in rheumatoid arthritis (RA). Because methotrexate (MTX) is the first-line therapy in RA, we studied whether MRP8/14 levels can predict response to MTX.

Authors : Patro Pradeepta Sekhar, Singh Ankita, Misra Ramnath, Aggarwal Amita,

(7) Myeloid-related proteins-8 and -14 are expressed but dispensable in the pathogenesis of experimental epidermolysis bullosa acquisita and bullous pemphigoid.[TOP]

Pubmed ID :26692467
Publication Date : //
Myeloid-related protein-8 (MRP-8) and its heterodimeric partner, MRP-14 belong to the group of danger-associated molecular patterns (DAMPs) and are associated with numerous chronic human disorders. However, their functional role in autoimmunity remains largely unclear.

Authors : Akbarzadeh Reza, Yu Xinhua, Vogl Thomas, Ludwig Ralf J, Schmidt Enno, Zillikens Detlef, Petersen Frank,

(8) Gout Is a Chronic Inflammatory Disease in Which High Levels of Interleukin-8 (CXCL8), Myeloid-Related Protein 8/Myeloid-Related Protein 14 Complex, and an Altered Proteome Are Associated With Diabetes Mellitus and Cardiovascular Disease.[TOP]

Pubmed ID :26248007
Publication Date : //
The frequent association of gout with metabolic syndrome and cardiovascular disease (CVD) suggests that it has a systemic component. Our objective was to study whether circulating proinflammatory cytokines are associated with comorbidities in gout patients.

Authors : Kienhorst Laura B E, van Lochem Ellen, Kievit Wietske, Dalbeth Nicola, Merriman Marilyn E, Phipps-Green Amanda, Loof Arnoud, van Heerde Waander, Vermeulen Sita, Stamp Lisa K, van Koolwijk Elly, de Graaf Jacqueline, Holzinger Dirk, Roth Johannes, Janssens Hein J E M, Merriman Tony R, Broen Jasper C A, Janssen Matthijs, Radstake Timothy R D J,

(9) Single amino acid charge switch defines clinically distinct proline-serine-threonine phosphatase-interacting protein 1 (PSTPIP1)-associated inflammatory diseases.[TOP]

Pubmed ID :26025129
Publication Date : //
Hyperzincemia and hypercalprotectinemia (Hz/Hc) is a distinct autoinflammatory entity involving extremely high serum concentrations of the proinflammatory alarmin myeloid-related protein (MRP) 8/14 (S100A8/S100A9 and calprotectin).

Authors : Holzinger Dirk, Fassl Selina Kathleen, de Jager Wilco, Lohse Peter, Röhrig Ute F, Gattorno Marco, Omenetti Alessia, Chiesa Sabrina, Schena Francesca, Austermann Judith, Vogl Thomas, Kuhns Douglas B, Holland Steven M, Rodríguez-Gallego Carlos, López-Almaraz Ricardo, Arostegui Juan I, Colino Elena, Roldan Rosa, Fessatou Smaragdi, Isidor Bertrand, Poignant Sylvaine, Ito Koichi, Epple Hans-Joerg, Bernstein Jonathan A, Jeng Michael, Frankovich Jennifer, Lionetti Geraldina, Church Joseph A, Ong Peck Y, LaPlant Mona, Abinun Mario, Skinner Rod, Bigley Venetia, Sachs Ulrich J, Hinze Claas, Hoppenreijs Esther, Ehrchen Jan, Foell Dirk, Chae Jae Jin, Ombrello Amanda, Aksentijevich Ivona, Sunderkoetter Cord, Roth Johannes,

(10) Myeloid-related protein-14 deficiency promotes inflammation in staphylococcal pneumonia.[TOP]

Pubmed ID :25792636
Publication Date : //
Staphylococcus aureus has evolved as an important cause of pneumonia in both hospital and community settings. Staphylococcal lung infection can lead to overwhelming pulmonary inflammation. During infection, neutrophils release complexes of myeloid-related protein (MRP)8 and MRP14 (MRP8/14). MRP8/14 has been shown to exert pro-inflammatory and chemotactic activity, and to assist in the killing of S. aureus. In the current study we sought to determine the role of MRP8/14 in the host response during S. aureus pneumonia.Pneumonia was induced in wildtype and MRP14-deficient mice (mice unable to form MRP8/14) by intranasal inoculation of 1×10(7) CFU of S. aureus USA300. Mice were sacrificed at 6, 24, 48 or 72 h after infection for analyses.S. aureus pneumonia was associated with a strong rise in MRP8/14 in bronchoalveolar lavage fluid and lung tissue. Surprisingly, MRP14 deficiency had a limited effect on bacterial clearance and was associated with increased cytokine levels in bronchoalveolar lavage fluid and aggravated lung histopathology. MRP14 deficiency in addition was associated with a diminished transmigration of neutrophils into bronchoalveolar lavage fluid at late time-points after infection together with reduced release of nucleosomes.MRP8/14 serves in an unexpected protective role for the lung in staphylococcal pneumonia.

Authors : Achouiti Ahmed, Vogl Thomas, Van der Meer Anne J, Stroo Ingrid, Florquin Sandrine, de Boer Onno J, Roth Johannes, Zeerleder Sacha, van 't Veer Cornelis, de Vos Alex F, van der Poll Tom,