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ELISA Kit for Canine Nitric Oxide Synthase 3,Endothelial(NOS3)

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[#E90868Ca] ELISA Kit for Canine Nitric Oxide Synthase 3,Endothelial(NOS3)


E90868Ca | ELISA Kit for Canine Nitric Oxide Synthase 3,Endothelial(NOS3), 96T
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(1) Investigation of the Applicability of a Rapid Diagnosis Test in the Diagnosis of Cystic Echinococcosis.[TOP]

Pubmed ID :29780006
Publication Date : //
Echinococcus granulosus, the etiological agent of cystic echinococcosis (CE) in humans and livestock, is a widely distributed zoonotic pathogen tapeworm. The infection is transmitted to humans by the ingestion of E. granulosus eggs released in the feces of definitive hosts such as dogs. The larval stage of the parasite develops a slowly enlarging cyst in the visceral organs, particularly in the liver and/or lung. The aim of the present study was to evaluate the diagnostic value of an immunochromatographic test (ICT) for CE.

Authors : Ertuğ Sema, Çalışkan Serçin Özlem, Malatyalı Erdoğan, Ertabaklar Hatice,

(2) Comparison of six commercial antigen kits for detection of Dirofilaria immitis infections in canines with necropsy-confirmed heartworm status.[TOP]

Pubmed ID :29657005
Publication Date : //
Patient-side test kits for detecting antigenemia in dogs associated with sexually mature female heartworms (Dirofilaria immitis) have been available for three decades, and these tests are continually updated and improved. To define the sensitivity (Se) and specificity (Sp) of contemporary antigen detection tests against cardiopulmonary D. immitis burden, we evaluated five patient-side kits-Anigen Rapid One Step (Bio note), SNAP 4Dx Plus Test Kit (IDEXX), WITNESS Heartworm Canine Heartworm Antigen Test Kit (Zoetis), VetScan Canine Heartworm Rapid Test (Abaxis), and Solo Step CH Canine Heartworm Antigen Test (Heska), and one microplate ELISA (DiroCHEK; Zoetis), using archived canine sera divided into five subclasses of female worms (0, 1-5, 6-20, 21-40, and >40). The patient-side tests were performed once, side-by-side according to each manufacturer's protocol by personnel blinded to the D. immitis status of each dog. The overall Se and Sp of the patient-side kits was ≥97.5 and =94.0%, respectively. For samples from dogs with 1-5, 6-20, and 21-40 D. immitis, the Se was between 96 and 100%, with a slight increase in Se in dogs with ≥41 worms. The agreement between tests for all subclasses of D. immitis burden was between 99 and 100%. The Se and Sp for the ELISA compared with the necropsy results of dogs was 99 and 96%, respectively. Agreement between each patient-side test and the ELISA was between 97 and 100%. All commercially available tests can give practitioners excellent patient-side information, allowing them to make informed decisions on the need for additional diagnostic work-up before instituting new or continuing D. immitis prophylaxis.

Authors : Henry Laura G, Brunson Katherine J, Walden Heather S, Wenzlow Nanny, Beachboard Sarah E, L Barr Kelli, Long Maureen T,

(3) Prevalence of Neospora spp. in donkeys in China.[TOP]

Pubmed ID :29557777
Publication Date : //
This study was conducted to detect specific anti-Neospora antibodies using a commercial competitive-inhibition ELISA kit, and to evaluate the risk factors for Neospora spp. infection. Out of a total of 2,228 donkey sera collected in three provinces in China, 211 (9.5%) were found to be positive for anti-Neospora antibodies. Statistical analysis revealed that age (p = 0.019, OR = 1.62, 95%CI: 1.08-2.44), feeding status (p < 0.001, OR = 3.79, 95%CI: 2.65-5.43), miscarriage history (p = 0.006, OR = 2.56, 95%CI: 1.27-4.01), and contact with dogs (p < 0.001, OR = 2.69, 95%CI: 1.86-3.88) were significant risk factors for Neospora spp. infection. This is the first evidence of Neospora infection in donkeys in China.

Authors : Cong Wei, Nie Lan-Bi, Qin Si-Yuan, Wang Wei-Li, Qian Ai-Dong, Meng Qing-Feng,

(4) Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach.[TOP]

Pubmed ID :29254723
Publication Date : //
Canine Monocytic Ehrlichiosis (CME) is a serious tick-borne rickettsial disease affecting canine populations globally. Besides few reports from stray and pet dogs from localised geographical regions (cities/towns/small states), a comprehensive study on prevalence of Ehrlichia canis (E. canis) among working dogs from different geo-climatic zones of India was pertinently lacking. Study of CME among these dog populations was thus carried out, encompassing clinical aspects and different diagnostic methodologies viz., microscopy, serology and molecular biology. During the two-year study period, clinical specimens from 225 cases suspected of canine ehrlichiosis were examined for clinical pathology and presence of the haemoparasites. Overall prevalence of ehrlichiosis by microscopic examination, commercial dot-ELISA kit and nested PCR assay was estimated to be 1.3%, 19.1% and 5.8%, respectively, which were found to be statistically significant by McNemar Chi squared test (p<0.05). It was also observed that possibly due to widespread use of doxycycline therapy in field, CME presently does not remain a potential threat which it uses to pose earlier. However, concurrent infections of E. canis and Babesia gibsoni were found to be mostly fatal. Keeping in view of high number of apparently healthy dogs (24) out of total positive cases (46) observed during the study, it is recommended that prevalence studies on CME should also involve screening of apparently healthy dogs. Phylogenetic analysis carried on partial sequencing of 16S rRNA of E. canis strains revealed that all of the Indian strains clustered in a single clade with other E. canis species from India and rest of the world. Molecular divergence was observed among the sequences of Brazilian and American isolates which were also included in the present study. These findings have thus opened a new paradigm for planning of pragmatic control strategies against CME.

Authors : Mittal Mitesh, Kundu Krishnendu, Chakravarti Soumendu, Mohapatra Jajati K, Nehra Kapil, Sinha V K, Sanjeeth B S, Churamani C P, Kumar Anil,

(5) Seroprevalence of toxoplasmosis and risk factors of Toxoplasma gondii infection among pregnant women in Sri Lanka: a cross sectional study.[TOP]

Pubmed ID :29202747
Publication Date : //
Toxoplasma gondii is an intracellular protozoan infecting humans and animals. Infection in adults usually causes mild disease but greater importance lies in preventing transplacental transmission which can cause major foetal anomalies and is vital to identify infection in pregnancy. Research on this regard in Sri Lanka is scarce and would be beneficial in developing antenatal care strategies for improved foetal outcome.

Authors : Iddawela Devika, Vithana Sanura Malinda Pallegoda, Ratnayake Chathura,

(6) Comparative evaluation of commercially available point-of-care heartworm antigen tests using well-characterized canine plasma samples.[TOP]

Pubmed ID :29143666
Publication Date : //
Dirofilaria immitis is a worldwide parasite that is endemic in many parts of the United States. There are many commercial assays available for the detection of D. immitis antigen, one of which was modified and has reentered the market. Our objective was to compare the recently reintroduced Witness® Heartworm (HW) Antigen test Kit (Zoetis, Florham Park, NJ) and the SNAP® Heartworm RT (IDEXX Laboratories, Inc., Westbrook, ME) to the well-based ELISA DiroChek® Heartworm Antigen Test Kit (Zoetis, Florham Park, NJ).

Authors : Starkey Lindsay A, Bowles Joy V, Payton Mark E, Blagburn Byron L,

(7) Seroprevalence of Neospora caninum in Dairy Cattle Raised in Çiçekdaği District of Kırşehir Province.[TOP]

Pubmed ID :29035240
Publication Date : //
Neospora caninum is one of the most important causes of abortion in cattle worldwide and causes significant economic losses in the meat and dairy industries. This study aimed to determine the seroprevalence of N. caninum in dairy cattle raised in Çiçekdağı district of Kırşehir province.

Authors : Yıldız Kader, Gökpınar Sami, Sürsal Neslihan, Değirmenci Rukiye,

(8) Epidemiological survey of ticks and tick-borne pathogens in pet dogs in south-eastern China.[TOP]

Pubmed ID :28971797
Publication Date : //
To understand the epidemiology of tick infestation and tick-borne diseases in pet dogs in south-eastern China and to develop a reference for their prevention and treatment, we collected 1550 ticks parasitizing 562 dogs in 122 veterinary clinics from 20 cities of south-eastern China. Dogs were tested for common tick-borne pathogens; collected ticks were identified and processed for the detection of tick-borne pathogens. The use of an in vitro ELISA diagnostic kit for antibody detection (SNAP®4Dx® Plus) on dog sera found the infection rates with Borrelia burgdorferi sensu lato, Ehrlichia canis, and Anaplasma spp. to be 0.4%, 1.3% and 2.7%, respectively. By using a specific ELISA method, the infection rate with Babesia gibsoni was 3.9%. Rhipicephalus sanguineus sensu lato, Haemaphysalis longicornis and Rhipicephalus haemaphysaloides were the major tick species identified on pet dogs. PCR tests were conducted to detect five tick-borne pathogens in 617 ticks. The infection rate was 10.2% for E. canis, 3.4% for Anaplasma platys, 2.3% for B. gibsoni, 0.3% for B. burgdorferi s.l. and 0% for Babesia canis. Some ticks were co-infected with two (1.46%) or three pathogens (0.16%). These results indicate the infestation of pet dogs by ticks infected with tick-borne pathogens in south-eastern China, and the need for effective treatment and routine prevention of tick infestations in dogs.

Authors : Zhang Jianwei, Liu Qingbiao, Wang Demou, Li Wanmeng, Beugnet Frédéric, Zhou Jinlin,

(9) Effects of Nano-Hydroxyapatite/Polyetheretherketone-Coated, Sandblasted, Large-Grit, and Acid-Etched Implants on Inflammatory Cytokines and Osseointegration in a Peri-Implantitis Model in Beagle Dogs.[TOP]

Pubmed ID :28945699
Publication Date : //
BACKGROUND This study explored the effects of nano-hydroxyapatite/polyetheretherketone (n-HA/PEEK)- coated sandblasted, large-grit, and acid-etched (SLA) implants on inflammatory cytokines and osseointegration in peri-implantitis model beagle dogs. MATERIAL AND METHODS Peri-implantitis models were established. Eight beagle dogs were randomly and evenly assigned into SLA tied, SLA + n-HA/PEEK tied, SLA untied, or SLA + n-HA/PEEK untied groups. A special periodontal probe was used to detect the plaque index (PLI), probing depth (PD), and modified Sulcus Bleeding Index (mSBI). Gingival crevicular fluid was collected and an ELISA kit was utilized to detect IL-1, IL-6, and IL-17 levels. The colony-forming units were counted and the maximum shear strength of implants was tested using the axial pullout test. HE staining was used to detect the inflammation of peri-implant bone tissues. Osseointegration was observed through toluidine blue staining. Bone-to-implant contact (BIC) was obtained through histological observation and the mineral apposition rate (MAR) was calculated after immune fluorescent double staining. RESULTS The SLA tied group demonstrated higher levels of PLI, PD, mSBI, IL-1, IL-6, and IL-17 and a higher degree of inflammation than the SLA + n-HA/PEEK tied group. The tied groups also displayed similar results over the untied groups at the same time point. The maximum shear strength, BIC, and MAR in the SLA tied group were significantly lower than in the SLA + n-HA/PEEK tied group. CONCLUSIONS Our findings demonstrate that SLA + n-HA/PEEK implants can promote osseointegration and relieve the inflammation response of peri-implantitis in beagle dogs.

Authors : Yang Hua-Wei, Tang Xiao-Shan, Tian Zhuo-Wei, Wang Yang, Yang Wen-Yi, Hu Jing-Zhou,

(10) Pentraxin-3 Levels in Gingival Crevicular Fluid during Canine Retraction with Nickel-Titanium Coil Spring and Active Tieback.[TOP]

Pubmed ID :28816195
Publication Date : //
Orthodontic treatment is routinely carried out in patients with the purpose of correcting various forms of dental malocclusions. Retraction of the canines can be achieved either individually or along with incisor. Pentraxin-3 (PTX-3) is regarded as the true independent indicator of disease activity. Hence, we undertook the present study to assess and compare the level of PTX-3 in patients undergoing canine retraction with active tieback and Nickel-Titanium (NiTi) coil spring.

Authors : Thukral Rakesh, Mangat Sukhpreet, Ganguly Anvesha, Agarkar Sanket S, Bali Harleen, Grover Shekhar,