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IgG, Bovine; ELISA

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[#YSRTPBP002] IgG, Bovine; ELISA

Related Publications :

(1) Vaccination with killed whole-cells of Escherichia coli O157:H7 hha mutant emulsified with an adjuvant induced vaccine strain-specific serum antibodies and reduced E. coli O157:H7 fecal shedding in cattle.
(2) Systemic humoral immunity in beef bulls following therapeutic vaccination against Tritrichomonas foetus.
(3) Ultrasonic pretreatment combined with dry-state glycation reduced the IgE/IgG-binding ability of α-lactalbumin revealed by high-resolution mass spectrometry.
(4) Squid type II collagen as a novel biomaterial: Isolation, characterization, immunogenicity and relieving effect on degenerative osteoarthritis via inhibiting STAT1 signaling in pro-inflammatory macrophages.
(5) Two-Dimensional MoS-Based Zwitterionic Hydrophilic Interaction Liquid Chromatography Material for the Specific Enrichment of Glycopeptides.
(6) Immunogenicity of a Staphylococcus aureus-cholera toxin A/B vaccine for bovine mastitis.
(7) Assembly of Multifunctionalized Gold Nanoparticles with Chemiluminescent, Catalytic, and Immune Activity for Label-Free Immunoassays.
(8) Prevalence and risk factors of Rift Valley fever in humans and animals from Kabale district in Southwestern Uganda, 2016.
(9) Onchocerca - infected cattle produce strong antibody responses to excretory-secretory proteins released from adult male Onchocerca ochengi worms.
(10) High seroprevalence for Crimean-Congo haemorrhagic fever virus in ruminants in the absence of reported human cases in many regions of Bulgaria.

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(1) Vaccination with killed whole-cells of Escherichia coli O157:H7 hha mutant emulsified with an adjuvant induced vaccine strain-specific serum antibodies and reduced E. coli O157:H7 fecal shedding in cattle.[TOP]

Pubmed ID :29778196
Publication Date : //
Escherichia coli O157:H7 (O157) can cause from a mild diarrheal illness to hemorrhagic colitis and hemolytic uremic syndrome in humans. Cattle are the primary reservoir for O157 and fecal shedding of O157 by these animals is a major risk factor in contamination of cattle hides and carcasses at slaughter. Vaccination is an important strategy to reduce fecal shedding of O157 in cattle. In this study, we evaluated the immunogenicity and efficacy of an inactivated vaccine strain of O157 formulated with an adjuvant. This vaccine strain was deleted of the hha gene enabling high level expression of the locus of enterocyte effacement (LEE) encoded proteins required for O157 colonization in cattle. The inactivated vaccine strain emulsified with the adjuvant or suspended in the phosphate-buffered saline (PBS) was injected in the neck muscles of two groups of weaned calves followed by a booster three weeks later with the corresponding formulation. Animals in groups 3 and 4 were injected similarly with the adjuvant and PBS, respectively. All animals were orally inoculated three weeks post-booster vaccination with a live culture of O157. The animals vaccinated with the adjuvanted vaccine showed higher serum antibody titers to the vaccine strain and shed O157 for a shorter duration and at lower numbers compared to the animals vaccinated with the non-adjuvanted vaccine, adjuvant-only, or PBS. Western blotting of the vaccine strain lysates showed higher immunoreactivity of serum IgG in vaccinated animals to several O157-specific proteins and lipopolysaccharides (LPS). The vaccination induced IgG showed specificity to LEE-encoded proteins and outer membrane LPS as LEE and waaL deletion mutants, unable to produce LEE proteins and synthesize high molecular weight LPS, respectively, yielded significantly lower antibody titers compared to the parent vaccine strain. The positive reactivity of the immune serum was also observed for purified LEE-encoded proteins EspA and EspB. In conclusion, the results of this animal study showed that a two-dose regimen of an adjuvanted vaccine is capable of inducing O157-specific immune response that directly or indirectly reduced fecal shedding of O157 in cattle.

Authors : Sharma Vijay K, Schaut Robert G, Loving Crystal L,



(2) Systemic humoral immunity in beef bulls following therapeutic vaccination against Tritrichomonas foetus.[TOP]

Pubmed ID :29773139
Publication Date : //
The utility of therapeutic vaccination of bulls against Tritrichomonas foetus has been advocated in previous studies, but anecdotal reports suggest this practice does not clear infections and may additionally confound diagnostic testing by reducing parasite burdens below detectable limits. The objective of this study was to characterize the systemic humoral immune response to therapeutic vaccination in T. foetus-infected bulls over a period of four months using an indirect ELISA and to compare the dynamics of this response to culture and PCR results to establish the existence of a relationship (or lack thereof) between immunization and infection status. A study population of 4- to 6-year-old T. foetus-infected beef bulls (n = 20) was divided equally into a treatment group and a control group. The treatment group received two doses of commercially prepared whole cell killed vaccine 2 weeks apart while the control group received injections of vaccine diluent. Blood samples were collected at each injection and at 4 subsequent dates every 4 weeks thereafter (i.e. 0, 2, 6, 10, 14, and 18 wks) to measure IgG and IgG antibody subisotype response via an indirect ELISA. Preputial smegma samples were collected at the four monthly intervals following vaccination for diagnosis of infection via InPouch™ culture, Modified Diamond's Medium (MDM) culture, and PCR. Humoral response for both IgG isotypes from week 2 through week 18 were significantly increased in vaccinates compared to controls. No significant decrease in infection prevalence was detected in the treatment group for any of the diagnostic methods used. The apparent lack of pathogen clearance during a stimulated immune response suggests that therapeutic vaccination may not be a useful T. foetus management practice.

Authors : Alling Christopher, Rae D Owen, Ma Xiaojie, Neumann Laura, Lollis L Gene, Steele Elizabeth, Yelvington John, Naikare Hemant K, Walden Heather Stockdale, Crews John, Boughton Raoul,



(3) Ultrasonic pretreatment combined with dry-state glycation reduced the IgE/IgG-binding ability of α-lactalbumin revealed by high-resolution mass spectrometry.[TOP]

Pubmed ID :29758985
Publication Date : //
Bovine α-lactalbumin (α-LA) is one of major food allergens in cow's milk. The present work sought to research the effects of ultrasonic pretreatment combined with dry heating-induced glycation between α-LA and galactose on the IgE/IgG-binding ability and glycation extent of α-LA, which determined by inhibition ELISA and high-resolution mass spectrometry respectively. The IgE/IgG-binding ability of glycated α-LA were significantly decreased as a result of ultrasonic pretreatment, while average molecular weight, incorporation ratio (IR) value, the location and number of glycation site, and degree of substitution per peptide (DSP) value was elevated. When the mixtures of α-LA and galactose pretreated by ultrasonication at 150 W/cm2, the glycated α-LA possess seven glycation sites, the highest IR and DSP value, and the lowest IgE/IgG-binding ability. Therefore, the decrease in IgE/IgG-binding ability of α-LA depend not only on the shielding effect of the linear epitope was found to be caused by the glycation of K13, K16, K58, K93 and K98 sites, but also on the intensified glycation extent, which reflected in the increase of IR value, the number of glycation sites and DSP value. Moreover, allergenic proteins and monosaccharides pretreated by ultrasonication then followed by dry-state glycation was revealed as a promising way of achieving lower allergenicity of proteins in food processing.

Authors : Liu Jun, Tu Zong-Cai, Liu Guang-Xian, Niu Chen-di, Yao Hong-Lin, Wang Hui, Sha Xiao-Mei, Shao Yan-Hong, Kaltashov Igor A,



(4) Squid type II collagen as a novel biomaterial: Isolation, characterization, immunogenicity and relieving effect on degenerative osteoarthritis via inhibiting STAT1 signaling in pro-inflammatory macrophages.[TOP]

Pubmed ID :29752100
Publication Date : //
Collagen from marine organisms has a broad prospect in biomedical field, yet the knowledge on marine-derived type II collagen is rare. Herein, a novel type II collagen was successfully isolated from squid cartilage for the first time. After being characterized, the immunogenicity of squid type II collagen (SCII) was evaluated and compared with that of bovine type II collagen (BCII). Then investigations were further conducted for the impacts of SCII on pro-inflammatory macrophages and macrophage chemotaxis. The degenerative osteoarthritis (OA) -relieving effects of SCII were explored using OA rat model in vivo. Our results demonstrated that the isolated SCII maintained triple-superhelical structure of native collagen with high purity. Different from BCII, SCII presented no immunogenicity since it neither induced abnormal proliferation of lymphocytes in vitro nor changed the basic levels of IgM, IgG, anti-type II collagen IgG and CD4/CD8 lymphocytes ratio in vivo. Additionally, SCII also exerted prominent anti-inflammatory effects. SCII significantly reduced the production of pro-inflammatory cytokines by enhancing the activity of TCPTP and subsequently prompting the dephosphorylation of p-STAT1 in pro-inflammatory macrophages. Besides, it indirectly prevented hypertrophic changes of chondrocytes, and markedly impeded chemotaxis of macrophages. Moreover, inflammation condition in OA rats was significantly alleviated under treatment with SCII. These data suggested that the newly developed SCII could not only avoid the immunogenic risks of collagen derived from terrestrial animals, but more importantly, provide new choice for the control and treatment of OA.

Authors : Dai Meilu, Liu Xin, Wang Nanping, Sun Jiao,



(5) Two-Dimensional MoS-Based Zwitterionic Hydrophilic Interaction Liquid Chromatography Material for the Specific Enrichment of Glycopeptides.[TOP]

Pubmed ID :29742898
Publication Date : //
Mass spectrometry (MS)-based glycoproteomics research requires highly efficient sample preparation to eliminate interference from non-glycopeptides and to improve the efficiency of glycopeptide detection. In this work, a novel MoS/Au-NP (gold nanoparticle)-L-cysteine nanocomposite was prepared for glycopeptide enrichment. The two-dimensional (2D) structured MoS nanosheets served as a matrix that could provide a large surface area for immobilizing hydrophilic groups (such as L-cysteine) with low steric hindrance between the materials and the glycopeptides. As a result, the novel nanomaterial possessed an excellent ability to capture glycopeptides. Compared to commercial zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) materials, the novel nanomaterials exhibited excellent enrichment performance with ultrahigh selectivity and sensitivity (approximately 10 fmol), high binding capacity (120 mg g), high enrichment recovery (more than 93%), satisfying batch-to-batch reproducibility, and good universality for glycopeptide enrichment. In addition, its outstanding specificity and efficiency for glycopeptide enrichment was confirmed by the detection of glycopeptides from an human serum immunoglobulin G (IgG) tryptic digest in quantities as low as a 1:1250 molar ratio of IgG tryptic digest to bovine serum albumin tryptic digest. The novel nanocomposites were further used for the analysis of complex samples, and 1920 glycopeptide backbones from 775 glycoproteins were identified in three replicate analyses of 50 μg of proteins extracted from HeLa cell exosomes. The resulting highly informative mass spectra indicated that this multifunctional nanomaterial-based enrichment method could be used as a promising tool for the in-depth and comprehensive characterization of glycoproteomes in MS-based glycoproteomics.

Authors : Xia Chaoshuang, Jiao Fenglong, Gao Fangyuan, Wang Heping, Lv Yayao, Shen Yehua, Zhang Yangjun, Qian Xiaohong,



(6) Immunogenicity of a Staphylococcus aureus-cholera toxin A/B vaccine for bovine mastitis.[TOP]

Pubmed ID :29739718
Publication Date : //
Staphylococcus aureus causes a chronic, contagious disease of the udder, or mastitis, in dairy cows. This infection is often refractory to antibiotic treatment, and has a significant economic impact on milk production worldwide. An effective vaccine to prevent S. aureus mastitis would improve animal health, reduce antibiotic dependence and inform human vaccine approaches. The iron-regulated surface determinant A (IsdA) and clumping factor A (ClfA) are conserved S. aureus extracellular-matrix adhesins and target vaccine antigens. Here we report the results of two bovine immunogenicity trials using purified IsdA and ClfA-cholera toxin A/B chimeras (IsdA-CTA/B and ClfA-CTA/B). Cows were intranasally inoculated with IsdA-CTA/B + ClfA-CTA/B at dry off and followed for 70 days. Trial 1 utilized three groups with one or two booster doses at a total concentration of 600 or 900 μg. Trial 2 utilized two groups with one booster at a total concentration of 1200 μg. Humoral immune responses in serum and milk were examined by ELISA. Responses in serum were significant between groups and provide evidence of antigen-specific IgG induction after vaccination in both trials. Cellular proliferation was detected by flow cytometry using antigen-stimulated PBMCs from day 60 of Trial 2 and revealed an increase in CD4+ T cells from vaccinated cows. IsdA and ClfA stimulation induced IL-4 expression, but not IFN-γ or IL-17, in PBMCs from day 60 as determined by cytokine expression analysis. Opsonophagocytosis of S. aureus confirmed the functional in vitro activity of anti-IsdA antibodies from Trial 2 serum and milk. The vaccine was well tolerated and safe, and results support the potential of mucosally-delivered CTA/B chimeras to protect cows from mastitis caused by S. aureus.

Authors : Misra N, Wines T F, Knopp C L, Hermann R, Bond L, Mitchell B, McGuire M A, Tinker J K,



(7) Assembly of Multifunctionalized Gold Nanoparticles with Chemiluminescent, Catalytic, and Immune Activity for Label-Free Immunoassays.[TOP]

Pubmed ID :29727158
Publication Date : //
In this study, we report a universal label-free immunoassay to detect antigen based on multifunctionalized gold nanoparticles (MF-GNPs), which were obtained by successive assembly of N-aminobutyl- N-ethylisoluminol functionalized gold nanoparticles (ABEI-GNPs) with antibody, bovine serum albumin (BSA) and Co. MF-GNPs exhibited excellent chemiluminescent (CL), catalytic and immune activity. It was demonstrated that the CL signal of MF-GNPs decreased in the presence of antigens via antigen-antibody specific binding using human immunoglobulin G (hIgG) and corresponding antibody goat anti-human IgG (anti-hIgG) as a model system, due to that immunoreaction led to the aggregation of GNPs. According to the decreased CL intensity, hIgG could be determined in the range of 1.0 fM to 1.0 nM with a low detection limit of 0.13 fM. Furthermore, this CL strategy was also confirmed to be a general one by replacing hIgG with heart-type fatty acid-binding protein (H-FABP), which is a biomarker of early acute myocardial infarction (AMI). The CL strategy could be employed to detect H-FABP ranging from 10.0 fM to 10.0 nM, and the detection limit is 7.8 fM. The CL strategy also showed good selectivity. It might be extended to detect other antigens if their corresponding antibodies are available.

Authors : Huang Yao, Gao Lingfeng, Cui Hua,



(8) Prevalence and risk factors of Rift Valley fever in humans and animals from Kabale district in Southwestern Uganda, 2016.[TOP]

Pubmed ID :29723189
Publication Date : //
Rift Valley fever (RVF) is a zoonotic disease caused by Rift Valley fever virus (RVFV) found in Africa and the Middle East. Outbreaks can cause extensive morbidity and mortality in humans and livestock. Following the diagnosis of two acute human RVF cases in Kabale district, Uganda, we conducted a serosurvey to estimate RVFV seroprevalence in humans and livestock and to identify associated risk factors.

Authors : Nyakarahuka Luke, de St Maurice Annabelle, Purpura Lawrence, Ervin Elizabeth, Balinandi Stephen, Tumusiime Alex, Kyondo Jackson, Mulei Sophia, Tusiime Patrick, Lutwama Julius, Klena John D, Brown Shelley, Knust Barbara, Rollin Pierre E, Nichol Stuart T, Shoemaker Trevor R,



(9) Onchocerca - infected cattle produce strong antibody responses to excretory-secretory proteins released from adult male Onchocerca ochengi worms.[TOP]

Pubmed ID :29716541
Publication Date : //
The front line molecules from filarial worms and other nematodes or helminthes are their Excretory-Secretory (ES) products. Their interaction with the host cells, proteins and immune system accounts for the skin and eye pathology or hyposensitivity observed in human onchocerciasis. ES products and adult worms' crude extracts from Onchocerca ochengi, a filarial nematode that infects the African zebu cattle, were utilized in the present study as a model for studying Onchocerca volvulus that causes river blindness in man.

Authors : Boursou Djafsia, Ndjonka Dieudonné, Eisenbarth Albert, Manchang Kingsley, Paguem Archille, Ngwasiri Nancy Ngwafu, Vildina Jacqueline Dikti, Abanda Babette, Krumkamp Ralf, van Hoorn Silke, Renz Alfons, Achukwi Mbunkah Daniel, Liebau Eva, Brattig Norbert W,



(10) High seroprevalence for Crimean-Congo haemorrhagic fever virus in ruminants in the absence of reported human cases in many regions of Bulgaria.[TOP]

Pubmed ID :29713918
Publication Date : //
Crimean-Congo haemorrhagic fever (CCHF) is a severe tick-borne zoonotic disease in humans caused by CCHF virus. It has been observed in Bulgaria since 1952 and over the years more than 1600 cases have been reported in the country. Close contact with viraemic livestock was shown as one of the main causes of the infection. Detection of CCHF virus specific antibodies in livestock can be used as an indicator for virus circulation and risk assessment. CCHF seroprevalence was investigated in 843 cattle, 88 goats and 130 sheep, originating from all 28 districts of Bulgaria. CCHF virus-specific IgG antibodies were observed in 165 cattle (19.6, 95% CI 17.0-22.4%), in 20 goats (22.7, 95% CI 15.2-32.5%) and in 10 sheep (7.7, 95% CI 4.2-13.6%). The highest seroprevalence was detected in the district of Kardzhali, South Bulgaria (86.7, 95% CI 73.8-93.7%), a well-known focus of CCHF in humans. The other two districts with human CCHF cases, Blagoevgrad and Burgas, located in southwest and southeast Bulgaria, showed overall seroprevalences in livestock of 41.9% (95% CI 28.4-56.7%) and 31.3% (95% CI 22.2-42.1%), respectively. Seroprevalences in districts with no history of human CCHF cases varied between 55% (95% CI 39.8-69.3%) and 22.5% (95% CI 12.3-37.5%). These results suggest frequent CCHF virus infections even in regions without human CCHF cases and an enhanced risk of infection for humans in close contact with the infected livestock.

Authors : Christova Iva, Panayotova Elitsa, Groschup Martin H, Trifonova Iva, Tchakarova Simona, Sas Miriam Andrada,