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ELISA Kit for Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2)

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[#E97122Hu] ELISA Kit for Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2)

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E97122Hu | ELISA Kit for Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2), 96T/Kit
More informations about ELISA Kit for Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) in Antibody-antibodies.com

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(1) Gp91phox (NOX2) in Activated Microglia Exacerbates Neuronal Damage Induced by Oxygen Glucose Deprivation and Hyperglycemia in an in Vitro Model.[TOP]

Pubmed ID :30317245
Publication Date : //
Peri-operative cerebral ischemia reperfusion injury is one of the most serious peri-operative complications that can be aggravated in patients with diabetes. A previous study showed that microglia NOX2 (a NADPH oxidase enzyme) may play an important role in this process. Here, we investigated whether increased microglial derived gp91phox, also known as NOX2, reduced oxygen glucose deprivation (OGD) after induction of hyperglycemia (HG).

Authors : Zeng Xianzhang, Ren Hongliang, Zhu Yana, Zhang Ruru, Xue Xinxin, Tao Tao, Xi Hongjie,



(2) Penehyclidine hydrochloride preconditioning provides pulmonary and systemic protection in a rat model of lung ischaemia reperfusion injury.[TOP]

Pubmed ID :30201378
Publication Date : //
Penehyclidine hydrochloride (PHC) is a new anticholinergic agent that provides protective effects in experimental models of heart and brain ischaemia as well as reperfusion (I/R) injury. In this study, we tested the hypothesis that PHC can alleviate lung ischaemia-reperfusion injury and improve pulmonary and systemic function in rats. PHC was administered intravenously at various doses (d= 0.1, 0.3, 1, 3 mg/kg) to I/R rats. We used six indicators, including lung function, histologic examination, pulmonary oedema, oxidative stress, inflammatory responses, and apoptosis staining to quantify the pulmonary and systemic protective effects of PHC. Haematoxylin and eosin staining was used for pulmonary histologic examination. The expression of Toll-like receptor (TLR) 4, phospho-inhibitor of NF-κB (p-IκB) and nuclear factor-kappa B (NF-κB) was analysed using western blotting. ELISA was conducted to detect inflammatory mediators. Oxidative stress markers as well as myeloperoxidase (MPO) were determined using an assay kit. PHC preconditioning (with concentrations ranging from 0.3 mg/kg to 3 mg/kg 30 min before the onset of I/R) significantly reduced lung histopathological changes, down regulated TLR4, p-IκB and NF-κB expression, and decreased inflammatory mediators as well as the total number of leukocytes and neutrophils in bronchoalveolar lavage (BAL) fluid and plasma. The lung tissue contents of reactive oxygen species (ROS), malondialdehyde (MDA), and MPO as well as pulmonary oedema formation decreased, while SOD (superoxide dismutase) activity was significantly upregulated. PHC preconditioning (with concentrations ranging from 1 mg/kg to 3 mg/kg) significantly improved the lung function and attenuated the apoptotic rate. The probable mechanism for this finding is the inhibition of proinflammatory mediators via the suppression of reactive oxygen species production and the TLR4/NF-κB signalling pathway.

Authors : Wang Yaguang, Lin Duomao, Tan Hongbao, Gao Yafen, Ma Jun,



(3) Seroprevalence and Associated Risk Factors of Hepatitis B Virus Infection Among Children in Enugu Metropolis.[TOP]

Pubmed ID :30150873
Publication Date : //
Though measures are being put in place for the management of Hepatitis B virus (HBV) infection in Nigeria, children remain the most vulnerable to develop chronic hepatitis. Routine screening in children is therefore necessary for effective control. However, the performance of the commonly used immunochromatographic test (ICT) strips has been challenging. Also, identifying the risk factors of transmission in this age group is of importance for the implementation of preventive measures. Hence, the goal of this study was to assess the test performance of the routinely used ICT strip and identify the associated clinical manifestations and risk factors of HBV.

Authors : Ezeilo Maryann Chinenye, Engwa Godwill Azeh, Iroha Romanus Ifeanyi, Odimegwu Damian Chukwu,



(4) MiRNA-206 suppresses PGE2-induced colorectal cancer cell proliferation, migration, and invasion by targetting TM4SF1.[TOP]

Pubmed ID :30135139
Publication Date : //
MiRNA (miR)-206 plays a tumor suppressor role in various cancer types. Here, we investigated whether miR-206 is involved in prostaglandin E2 (PGE2)-induced epithelial-mesenchymal transition (EMT) in colorectal cancer (CRC) cells through the targetting of transmembrane 4 L six family member 1 (TM4SF1).The effect of PGE2 on growth and apoptosis of CRC cells was evaluated using the MTT assay and flow cytometry analysis, respectively. TM4SF1 and miR-206 expression levels were determined with quantitative polymerase chain reaction (qRT-PCR) in CRC tissues and cell lines. The concentration of PGE2 in the serum of CRC patients and healthy controls was measured with an ELISA kit. A miR-206 or TM4SF1 construct was transfected into cells with PGE2. Transwell migration and invasion assays were used to examine cell migration and invasion properties. Additionally, a luciferase assay was performed to determine whether TM4SF1 was directly targetted by miR-206.We found that miR-206 was down-regulated and TM4SF1 was up-regulated in human CRC tissues and cell lines. Moreover, miR-206 was negatively correlated with TM4SF1 expression. Bioinformatics analysis and a luciferase reporter assay revealed that miR-206 directly targetted the 3'-untranslated region (UTR) of TM4SF1, and TM4SF1 expression was reduced by miR-206 overexpression at both the mRNA and protein levels. Additionally, PGE2 significantly suppressed the expression of miR-206 and increased the expression of TM4SF1 in CRC cells. PGE2 induction led to enhanced CRC cell proliferation, migration, and invasion. Moreover, the overexpression of miR-206 decreased CRC cell proliferation, migration, and invasion compared with control group in PGE2-induced cells, and these effects could be recovered by the overexpression of TM4SF1. Overexpression of miR-206 also suppressed the expression of β-catenin, VEGF, MMP-9, Snail, and Vimentin and enhanced E-cadherin expression in PGE2-induced cells. These results could be reversed by the overexpression of TM4SF1. At last, up-regulation of miR-206 suppressed expression of -AKT and -ERK by targetting TM4SF1 in PGE2-induced cells.Our results provide further evidence that miR-206 has a protective effect on PGE2-induced colon carcinogenesis.

Authors : Park Young Ran, Seo Seung Young, Kim Se Lim, Zhu Shi Mao, Chun Sungkun, Oh Jung-Mi, Lee Min Ro, Kim Seong Hun, Kim In Hee, Lee Seung Ok, Lee Soo Teik, Kim Sang Wook,



(5) The possible impact of hyperuricemia on serum soluble receptor for advanced glycation end products (sRAGE) levels in teenagers: a case control study.[TOP]

Pubmed ID :30101695
Publication Date : //
Background Dietary advanced glycation end products (AGEs) and their interactions with the soluble receptors for AGEs (RAGE) play a crucial role in the pathogenesis of cardiovascular diseases. Objective This study was set out to assess, whether there was any association between serum sRAGE level and serum uric acid level in children with hyperuricemia Methods This case control study involved 53 patients (12 girls, 41 boys) with hyperuricemia (defined as serum uric acid >4.8 and >5.5 mg/dl in girls and boys, respectively) aged median of 13.5-15.5 years. Thirty-six healthy individuals with normal serum uric acid level were selected as a reference group. Additionally the study group with hyperuricemia was divided into two groups: HU-HT (hypertensive n=25) and HU-NT (normotensive n=28) teenagers. The serum concentration of human sRAGE was measured using a commercially available enzyme-linked immunosorbent assay (ELISA) kit. Results We found statistically significant differences in serum sRAGE levels between normotensive subjects with hyperuricemia median: 169.8 pg/ml (Q1-Q3:148.3-231.1 pg/ml) and reference group median: 129 pg/ml (Q1-Q3:107.4-175.3 pg/ml), p<0.01. Univariate analysis of the data revealed positive correlation between serum sRAGE and serum uric acid (r=0.306, p<0.05). Conclusion Our data showed that adolescents with an increased serum uric acid level had higher serum sRAGE levels. One of the possible explanation is of the oxidative stress, which plays an important role in pathogenesis of inflammation in patients with hyperuricemia. A question arises whether serum sRAGE might be a sensitive marker of inflammation process caused by hyperuricemia. Further studies are required to assess the relationship between sRAGE and markers of endothelial dysfunction.

Authors : Lemiesz M, Tenderenda-Banasiuk E, Sosnowska D, Rybi-Szuminska A, Storonowicz J, Lemiesz T, Wasilewska A,



(6) The effect of NAD-299 and TCB-2 on learning and memory, hippocampal BDNF levels and amyloid plaques in Streptozotocin-induced memory deficits in male rats.[TOP]

Pubmed ID :30027497
Publication Date : //
Alzheimer's disease (AD) is the most common form of dementia characterized by a progressive decline in cognitive function. The serotonergic system via the 5-HT1A receptor and 5-HT2A receptor is proposed to affect the cognitive process.

Authors : Afshar Simin, Shahidi Siamak, Rohani Ali Haeri, Komaki Alireza, Asl Sara Soleimani,



(7) Reflection and observation: cell-based screening failing to detect HBV in HUMSCs derived from HBV-infected mothers underscores the importance of more stringent donor eligibility to reduce risk of transmission of infectious diseases for stem cell-based medical products.[TOP]

Pubmed ID :29973264
Publication Date : //
In cell-based therapy, the transmission of communicable diseases imposes a substantial threat to recipients. In this study, we investigated whether cell-based screening could detect hepatitis B virus (HBV) in human umbilical cord-derived mesenchymal stem cells (HUMSCs) isolated from HBV-infected donors to understand the susceptibility of HUMSCs to HBV infection.

Authors : Liu Wei, Xie Yuanyuan, Gao Tianyun, Huang Feifei, Wang Liudi, Ding Lijun, Wang Wenqing, Liu Shuo, Dai Jianwu, Wang Bin,



(8) Detection of pregnancy in sheep using an ELISA for pregnancy-specific protein B.[TOP]

Pubmed ID :29958526
Publication Date : //
The early detection of pregnancy and the determination of fetal numbers have economic benefits in sheep production because of the seasonal breeding patterns where missing a breeding opportunity means the loss of one productive year. The purpose of this study was to evaluate the efficacy of the B6-HRP ELISA for ovine pregnancy-specific protein B (oPSPB) measurement in the detection of pregnancy and estimation of fetal numbers in different sheep breeds. BioPRYN® ELISA assay kit was used for the detection of pregnancy in the experimental animals. Ninety-three ewes of three breeds (British Milksheep - BM, Lacaune - L and Transylvanian Racka - TR), each from three farms in Hungary, were included in the study. BM and L ewes were artificially inseminated (AI). Thirty-five days after AI, all ewes were examined by transabdominal ultrasound. The TR flock was mated naturally over a six-week period. At the end of the mating period, the ewes were similarly examined by ultrasound. Blood samples were taken from all pregnant ewes twice (35 and 65 days after AI), and serum samples were assayed by the BioPRYN test. It can be concluded that the detection of serum PSPB by ELISA is a much easier, safer, less expensive and highly accurate method for the detection of ovine pregnancy. Although some breed-related differences were detectable at 35 and 65 days post breeding, no differences in oPSPB levels were found in pregnant ewes carrying different numbers of fetuses.

Authors : Milisits-Németh Tímea, Balogh Orsolya Gabriella, Egerszegi István, Kern László, Sasser R Garth, Gábor György,



(9) [Seroprevalence of pertussis toxin antibody in Manisa province of Turkey, after six years implementation of acellular pertussis vaccine].[TOP]

Pubmed ID :29933735
Publication Date : //
Vaccination is the most effective way of preventing pertussis disease. Turkey commenced a routine infant immunization program using whole cell (wP) pertussis vaccine in 1968. Immunization accelerated in 1985 after participation of Turkey in the Expanded Programme on Immunization initiated by the World Health Organization. Acellular vaccine (aP) replaced wP in 2008 and a booster was added to age 6 in 2010. The immunization programme was successful in reducing the morbidity rate from 20.58 per 100.000 in 1970 to the lowest level of 0.01 per 100.000 in 2009. However, reduction of vaccine-induced protection and reduced natural boosting of circulating Bordetella pertussis are likely to increase the susceptibility of the population. As a result, morbidity rate increased from 0.09 per 100.000 to 0.41 per 100.000 in 2015 compared to the previous year. The aim of this epidemiological study was to determine the seroprevalence of pertussis toxin (PT) antibodies among healthy people and its association with various social determinants in Manisa province in Turkey, 6 years after aP replaced wP vaccine. The study was conducted as a cross-sectional study with a sample of 1250 people that was randomly selected from the over 2 years of age population in Manisa in 2014. Seroprevalence of PT antibody was determined as the dependent variable of the study. Independent variables of the study were; gender, age, migration in the last 5 years, occupational class, perceived income, house ownership, number of people per room, annually per capita equivalent income. The presence of anti-PT IgG was detected by quantitatively using a commercially available ELISA kit. The antibody levels were categorized into groups according to pertussis infection or vaccination immune response status. The groups consisted of undetectable (< 5 IU/ml), mid-range (5-< 62.5 IU/ml: more than one year previously), high (62.5-< 125: with in 12 months) and very high (≥ 125 IU/ml: with in 6 months) antibody levels. The test results with ≥ 5 IU/ml were defined as seropositive. Level > 100 IU/ml detected among adolescent and adult participants indicated acute or recently recovered pertussis infection. Chi-square test was used to evaluate association between social determinants and pertussis seropositivity. The seroprevalence of the whole study population was 58.1% (95% CI 55.32-60.79) and no association was found with any of the social determinants. The highest seroprevalence was found among 2-9 age group (68.3%) followed by 70-79 age group (63.5%). The lowest seroprevalence was found among 20-29 age group (50.9%) followed by 10-19 age group (51.6%). When seropositivity levels according to ages were compared, it was found that there was a decrease one year after the first vaccination at 2nd, 4th and 6th months and the booster at the 6th year, with a lowest rate (19%) in 11 year-old. The highest seropositivity (77.3%) with a level of >100 IU/ml (13.6%) were detected at age 15 among all adolescent and adult participants. Adding an adolescent booster to immunization schedule and recommendation of vaccine to elderly people should be considered to reduce the incidence of pertussis disease in Turkey.

Authors : Özbek Özgen Alpay, Öktem İbrahim Mehmet Ali, Hekimoğlu Can Hüseyin, Sekreter Özgür, Emek Mestan, Atasoylu Gonca, Açıkgöz Ayla, Demirpençe Nur, Ceylan Ali, Baykal Elif Sanem, Ünal Belgin,



(10) Prevalence of Antibody against Angiotensin II Type 1 Receptor (AT1R) Among Thai Kidney Transplant Patients.[TOP]

Pubmed ID :29880351
Publication Date : //
Non-HLA antibodies specific to angiotensin II type 1 receptor (AT1R-Ab) are associated with antibody-mediated rejection after kidney transplantation. This study aimed to determine the prevalence of AT1R-Ab among pre-transplantation Thai patients and to compare the association of patient demographics with AT1R-Ab levels.

Authors : Viboon S, Townamchai N, Phiancharoen S, Kupatawintu P, Dhitivat V, Nathalang O,