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ELISA Kit for Lymphocyte Antigen 96 (LY96)

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[#E97705Hu] ELISA Kit for Lymphocyte Antigen 96 (LY96)


E97705Hu | ELISA Kit for Lymphocyte Antigen 96 (LY96), 96T/Kit
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(1) Correlation between CTLA4 genetic polymorphisms, its serum protein level and the susceptibility to recurrent spontaneous abortion: A case-control study.[TOP]

Pubmed ID :30334961
Publication Date : //
Present study was aimed to detect the influence of cytotoxic T-lymphocyte associated protein 4 (CTLA4) gene polymorphisms for the risk of recurrent spontaneous abortion (RSA), as well as the serum level of CTLA4 protein in RSA patients.One hundred thirty-three RSA patients and 146 healthy persons were recruited in this case-control study. PCR-RFLP was used to genotype the CTLA4 gene polymorphisms both in case and control groups. Serum level of CTLA4 was detected by ELISA kit. Quantitative variables were compared by t test or Mann-Whitney U test between groups. Qualitative variables were evaluated by χ test or Fisher exact test. Association strength was expressed by odds ratios (ORs) and 95% confidence intervals (95% CIs).G allele of rs4553808 (P = .027, OR = 0.570, 95% CI = 0.345-0.942) and T allele of rs5742909 (P = .027, OR = 0.570, 95% CI = 0.345-0.942) were distinctly associated with reduced susceptibility of RSA. Distinctly negative association has been discovered between rs231775 AA genotype and RSA susceptibility (P = .040, OR = 0.427, 95% CI = 0.188-0.973). CTLA4 protein had significantly higher serum level in RSA patients than in healthy controls (P = .028). In RSA patients, AA genotype carriers had higher CTLA4 serum level than that GG genotype carriers (17.83 ± 6.35 ng/mL vs 10.41 ± 7.28 ng/mL, P = .039).Minor alleles of CTLA4 polymorphisms might inhibit the RSA susceptibility via upregulated the protein expression level.

Authors : Li Li, Liu Jia, Qin Shuang, Li Ruiman,

(2) Ark shell Scapharca broughtonii hemocyte response against Vibrio anguillarum challenge.[TOP]

Pubmed ID :30219385
Publication Date : //
Scapharca broughtonii is one of the most important Arcidae aquaculture species in the Asia-Pacific region. We aimed to investigate the immune responses of hemocytes from ark shell S. broughtonii hemolymph against pathogens. Hemocyte ultrastructure and immunological activity in response to Vibrio anguillarum challenge were observed by scanning and transmission electron microscopy. Before ultrastructure observation, we used the API ZYM semi-quantitative kit to evaluate the levels of hydrolytic enzymes in the plasma and hemocytes following V. anguillarum infection. An enzyme-linked immunosorbent assay kit was used to investigate the variation in the lysozyme activity and hemocytes following bacterial infection. The results showed that hemocytes were the main defense cells against bacterial infection, whereas plasma played a role in the transport and support of hemocytes. It was presumed that an important function of lysozymes and hydrolytic enzymes in lysosomes was for bacterial digestion. Three major types of hemocytes were observed, namely, red blood cells (RBCs), white blood cells (WBCs), and thrombocytes (TCs). Scanning electron microscopy showed that the normal RBCs appeared pie-shaped with 10 μm diameter and 4 μm central thickness, whereas WBCs were spherical in shape with varying sizes, 4-8 μm diameter, and included small lymphocytes. TCs were long, spindle-shaped, and 12-20 μm in length. The cell membrane surface was smooth and even for all cells before pathogen challenge. Under transmission electron microscopy, RBCs displayed a limited ability to devour and digest bacteria adherent to the cell surface following infection. Many hemoglobin particles were observed in the RBC cytoplasm. WBCs were very active against bacterial invasion and showed a strong ability to digest and decompose infected and wrapped V. anguillarum through phagocytosis and lysosome fusion. Digestive vacuoles rapidly became transparent and were thought to contain increasing quantities of pathogen-induced lysozymes. WBCs that devoured pathogenic bacteria were prone to deformation as well as adhesion to each other. TCs were rich in endoplasmic reticulum (ER) content in their cytoplasm and were widely connected in a net-shaped structure. Mitochondria in TCs formed clusters upon invasion of V. anguillarum in the hemolymph. TCs disintegrated to release the ER into the plasma to form a mesh that facilitated clotting. The ability of circulating hemocytes to quickly modify their morphologies and stainability suggests that S. broughtonii is endowed with highly dynamic hemocyte populations capable of coping with environmental changes and rapidly growing pathogens.

Authors : Zhou Liqing, Zhao Dan, Wu Biao, Sun Xiujun, Liu Zhihong, Zhao Feng, Lv Zhenming, Yang Aiguo, Zhao Qing, Zhang Gaowei, Ma Chunyan,

(3) Function of SLC7A7 in T-Cell Acute Lymphoblastic Leukemia.[TOP]

Pubmed ID :30025393
Publication Date : //
Y+LAT1 protein, encoded by the SLC7A7 gene (a member of the SLC7 family), forms the cationic amino acid transport system y+L (system y+L). This system transports cationic amino acids such as arginine and lysine out of the cell. Arginine, in particular, is critical for T-cell activation and function in the immune response.

Authors : Ji Xiaohui, Yang Xiaoyun, Wang Nan, Kang Meiyun, Wang Yaping, Rong Liucheng, Fang Yongjun, Xue Yao,

(4) Cytotoxic T- Lymphocyte Antigen-4 (CTLA4) Gene Expression and Urinary CTLA4 Levels in Idiopathic Nephrotic Syndrome.[TOP]

Pubmed ID :29968132
Publication Date : //
To detect Cytotoxic T- Lymphocyte Antigen-4 (CTLA4) single nucleotide polymorphisms (SNPs) at +49A/G (rs231775) and -318C/T (rs5742909) positions in children with idiopathic nephrotic syndrome (INS) and also assay urinary soluble CTLA4 (sCTLA4) levels in children with minimal change disease (MCD), focal segmental glomerulosclerosis (FSGS) and steroid sensitive nephrotic syndrome (SSNS) in remission.

Authors : Mishra Om P, Chhabra Prashant, Narayan Gopeshwar, Srivastava Pradeep, Prasad Rajniti, Singh Ankur, Abhinay Abhishek, Batra Vineeta V,

(5) Hesperidin inhibits insulin-induced phosphoinositide 3-kinase/Akt activation in human pre-B cell line NALM-6.[TOP]

Pubmed ID :29893306
Publication Date : //
It has been shown that hesperidin induces apoptosis in NALM-6 cells through inhibition of nuclear factor-kappa B (NF-κB) activation.

Authors : Shahbazi Roghayeh, Cheraghpour Makan, Homayounfar Reza, Nazari Maryam, Nasrollahzadeh Javad, Davoodi Sayed Hossein,

(6) Enhanced Suppressive Activity of Regulatory T Cells in the Microenvironment of Malignant Pleural Effusions.[TOP]

Pubmed ID :29785404
Publication Date : //
Cancer metastatic spread to serous cavity causes malignant pleural effusions (MPEs), indicating dismal prognosis. Tumor microenvironment can implement suppressive activity on host immune responses. Thus, we investigated the prevalence of Tregs and the relationship between them and TGF- and IL-10 concentrations and measured expression of , , , and genes, as well as protein expression of selected genes in benign effusions and MPEs. The percentage of Tregs was determined by means of multicolor flow cytometry system. TGF- and IL-10 concentrations were measured using human TGF-1 and IL-10 ELISA kit. Relative mRNA expression of studied genes was analyzed by real-time PCR. The frequency of Tregs was significantly higher in MPEs compared to benign effusions; however, the level of TGF- and IL-10 in analyzed groups was comparable, and no correlation between concentrations of TGF- and IL-10 and percentage of Tregs was observed. Relative mRNA expression of all the genes was higher in CD4CD25 compared to CD4CD25 cells. In CD4CD25 cells from MPEs, relative mRNA expression of , , and genes was significantly higher than in benign effusions; however, the level of CD4CD25CTLA-4 cells in analyzed groups showed no significant differences. We found numerous genes correlations in an entire CD4CD25 cell subset and CD4CD25 cells from MPEs. Enhanced suppressive activity of Tregs is observed in the microenvironment of MPEs. Understanding of relations between cellular and cytokine immunosuppressive factors in tumor microenvironment may determine success of anticancer response.

Authors : Budna Joanna, Kaczmarek Mariusz, Kolecka-Bednarczyk Agata, Spychalski Łukasz, Zawierucha Piotr, Goździk-Spychalska Joanna, Nowicki Michał, Batura-Gabryel Halina, Sikora Jan,

(7) Circulating soluble RAGE and cell surface RAGE on peripheral blood mononuclear cells in healthy children.[TOP]

Pubmed ID :29723156
Publication Date : //
The receptor for advanced glycation end products (RAGE) has a critical role in the pathogenesis of inflammation. In healthy children, its basal expression on the peripheral blood mononuclear cell (PBMC) and the basal circulating soluble RAGE (sRAGE) levels are unknown. The aim of this study was to describe both.

Authors : García-Salido Alberto, Melen Gustavo, Gómez-Piña Vanesa, Oñoro-Otero Gonzalo, Serrano-González Ana, Casado-Flores Juan, Ramírez Manuel,

(8) Different profile of cytokine production in patients with systemic sclerosis and association with clinical manifestations.[TOP]

Pubmed ID :29601941
Publication Date : //
Immune dysregulation is a central process in the pathogenesis of systemic sclerosis (SSc). Cytokines produced by lymphocytes and monocytes are important mediators and induce tissue damage, recruit additional inflammatory cells, and promote extracellular matrix production and fibrosis. In the present research, we aimed to study the associations between levels of cytokines in serum and culture supernatants from peripheral blood mononuclear cells (PBMCs) and clinical manifestations in SSc patients. Serum samples were obtained from 56 SSc patients and 56 unrelated age- and gender-matched healthy individuals. Resting and anti-CD3/CD28-stimulated PBMC cultures were obtained from 19 SSc patients and 8 healthy controls. IL-2, IL-4, IL-6, IL-10, IL-17A, TNF, and IFN-γ levels were measured by ELISA or CBA. Serum cytokines, except IL-17A, were below the kit detection limit in most of the patients and controls. In unstimulated PBMC, the production of TNF(p = 0.004), IL-10(p = .048), IL-2(p < 0.001), and IL-6 (p = 0.01) was higher in SSc patients than in healthy controls. After anti-CD3/CD28 stimulation, scleroderma PBMCs had lower concentrations of TNF(p = 0.009), IL-10(p = .018), and IL-2(p = .002) than HC. In unstimulated PBMC, IL-2 concentration was higher in patients with esophageal dysmotility (p = 0.04), and IL-10 levels had a positive correlation with modified Rodnan score (p = 0.03). After anti-CD3/CD28 stimulation, higher levels of IL-2 and IL-4 were observed in SSc patients with lung fibrosis (p = 0.01 and 0.006, respectively), and higher levels of IL-10 (p = 0.04) and IL-4 (p = 0.04) in patients with digital ulcers. In conclusion, SSc patients have a different profile of cytokine production and this was associated with clinical manifestations.

Authors : Dantas Andréa Tavares, Almeida Anderson Rodrigues de, Sampaio Maria Clara Pinheiro Duarte, Cordeiro Marina Ferraz, Oliveira Priscilla Stela Santana de, Mariz Henrique de Ataíde, Pereira Michelly Cristiny, Rego Moacyr Jesus Barreto de Melo, Pitta Ivan da Rocha, Duarte Angela Luzia Branco Pinto, Pitta Maíra Galdino da Rocha,

(9) Serum IL-6, IL-23 profile and Treg/Th17 peripheral cell populations in pediatric patients with inflammatory bowel disease.[TOP]

Pubmed ID :29441874
Publication Date : //
IL-6 and IL-23 are both pleiotropic cytokines involved in the regulation of the immune response, inflammation, and hematopoeisis. They also could mediate effector cells and tolerance mediated by cells with regulatory function. Inflammatory bowel disease (IBD) is associated with a reduced ratio of Treg cells ato Th17 effector cells in peripheral blood and is characterised by a pro-inflammatory cytokine microenvironment which supports the continued generation of Th17 cells. It is well described in adults but little is known in a pediatric population. This study was aimed to investigate the role of IL-6, IL-23 and its association with Treg and Th17 subsets in pediatric IBD patients. Peripheral blood mononuclear cells from patients and controls were stimulated with PMA, ionomycin, and brefeldin A. The frequencies of CD4+Foxp3+ cells, and CD4+IL17a+ cells were analyzed by flow cytometry. The serum level of IL-6 and IL-23 was determined by Elisa kit. The mRNA expression of Foxp3, IL-17a, IL-6 and IL-23 was detected by real-time quantitative PCR. The ratio of Treg/Th17 decreased in pediatric IBD patients, and it strongly correlated with IL-6 and IL-23. The present study provides a quantitative analysis regarding the Th17/Treg cell balance in peripheral blood of children with IBD and its association with serum IL-6 and IL-23 level.

Authors : Zhu Xiao-Min, Shi Ying-Zuo, Cheng Ming, Wang Da-Feng, Fan Jian-Feng,

(10) Low prevalence of HCV infection with predominance of genotype 4 among HIV patients living in Libreville, Gabon.[TOP]

Pubmed ID :29385148
Publication Date : //
Gabon is an endemic area for human immunodeficiency virus (HIV) and hepatitis C virus (HCV) and the risk of co-infection is high.

Authors : Ndjoyi-Mbiguino Angélique, Kombe Kombe Arnaud John, Bivigou-Mboumba Berthold, Zoa-Assoumou Samira, Akombi Falone Larissa, Nzengui Nzengui Francis, M'boyis Kamdem Hervé, François-Souquière Sandrine,