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ELISA Kit for Early Prostate Cancer Antigen 2 (EPCA2)

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[#E84215Hu] ELISA Kit for Early Prostate Cancer Antigen 2 (EPCA2)

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E84215Hu | ELISA Kit for Early Prostate Cancer Antigen 2 (EPCA2) , 96T/Kit
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(1) Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury.[TOP]

Pubmed ID :29791311
Publication Date : //
Introduction The aim of the present study was to survey the protective effect of pretreatment with Persian honey on amelioration of side effects of chemotherapy and ischemia/reperfusion induced testicular injury. Materials and methods Forty adult's male wistar rats were divided into four groups of ischemia-reperfusion (IR), honey + ischemia-reperfusion (HIR), Busulfan (B) and Busulfan intraperitoneally+ honey (BH). The seminiferous tubules were rated for their modified spermatogenesis index (SI) by Johnsons score. Detection of single- and double-stranded DNA breaks at the early stages of apoptosis was performed using the in-situ cell death detection kit. Total serum concentration of Follicle-stimulating hormone (FSH) , Luteinizing hormone (LH) and testosterone was measured using ELISA. All data were expressed as mean ± SD and significance was set at p≤0.05. Results Honey improved SI in the HIR and BH groups and serum levels of FSH and LH in the BH and HIR groups (p<0.001). Also, serum levels of testosterone were significantly higher in BH and HIR groups. But, apoptotic cells in IR and B groups significantly increased (p<0.001), while in HIR and BH groups, the number of apoptotic cells decreased and the positive cells of TUNEL (TdT-mediated dUTP-X nick end labelling) staining were detected in spermatocytes and spermatid. Discussion Pretreatment with honey protect testis against chemotherapy and testicular IR injury, increase FSH and LH and testosterone and decrease the cellular damage and apoptosis. Honey can decrease the side effects of chemotherapy on reproductive system and prevent sterility.

Authors : Gholami Mohammadreza, Abbaszadeh Abolfazl, Baharvand Parastoo, Hasanvand Afshin, Hasanvand Amin, Gharravi Anneh Mohammad,



(2) Evaluation of an immunochromatography rapid diagnosis kit for detection of chikungunya virus antigen in India, a dengue-endemic country.[TOP]

Pubmed ID :29751761
Publication Date : //
Chikungunya virus (CHIKV) and dengue virus (DENV) are arboviruses that share the same Aedes mosquito vector, and there is much overlap in endemic areas. In India, co-infection with both viruses is often reported. Clinical manifestations of Chikungunya fever is often confused with dengue fever because clinical symptoms of both infections are similar. It is, therefore, difficult to differentiate from those of other febrile illnesses, especially dengue fever. We previously developed a CHIKV antigen detection immunochromatography (IC) rapid diagnosis kit [1]. The current study examined the efficacy of previously mentioned IC kit in India, a dengue-endemic country.

Authors : Jain Jaspreet, Okabayashi Tamaki, Kaur Navjot, Nakayama Emi, Shioda Tatsuo, Gaind Rajni, Kurosu Takeshi, Sunil Sujatha,



(3) Detection of early-stage extrahepatic cholangiocarcinoma in patients with biliary strictures by soluble B7-H4 in the bile.[TOP]

Pubmed ID :29736314
Publication Date : //
Increasing evidence has demonstrated that serum soluble B7-H4 (sB7-H4) is a useful tumour marker for cancer diagnosis and prognosis evaluation. Whether sB7-H4 is expressed in the bile of cholangiocarcinoma (CC) and is related to the progression of CC need to be explored. Bile sB7-H4 was obtained through endoscopic retrograde cholangiopancreatography (ERCP) from 213 patients with biliary strictures and detected was detected by a B7-H4 ELISA kit. Diagnostic value was compared among bile sB7-H4, CA19-9, CA12-5, CEA and ERCP-based cytological/tissue examination. Additionally, the correlations between the bile sB7-H4 concentration and the clinical characteristics of early-stage cholangiocarcinoma (ESCC) were studied. The bile sB7-H4 levels of patients with ESCC were significantly higher than in patients with benign biliary strictures (BBS) (<0.001). The receiver operating characteristic (ROC) curves of CA19-9, CA12-5 and CEA were 0.713, 0.554 and 0.451, respectively, were significantly lower than the ROC curves of bile sB7-H4 (0.837), the sensitivity of ERCP-based cytological/tissue examination was 57.5% and 68.4%, which was lower than that of bile sB7-H4 (81.7%) at cut-off value. A high level of bile sB7-H4 in patients with ESCC was found to be correlated with vascular invasion (<0.001), lymph node metastasis (<0.001) and TNM stage (=0.018), respectively. The overall survival rate (OS) of ESCC patients in the high sB7-H4 group was significantly lower than the OS of patients in the low sB7-H4 group (=0.009). Bile sB7-H4 could serve as a valuable biomarker for patients with ESCC and high levels of bile sB7-H4 correlate with poor clinical outcomes.

Authors : Ke Wenbo, Zeng Li, Hu Yunbi, Chen Sisi, Tian Min, Hu Qinggang,



(4) Use of low-level laser therapy on children aged 1 to 5 years with energy-protein malnutrition: A clinical trial.[TOP]

Pubmed ID :29703031
Publication Date : //
Episodes of malnutrition in early childhood can produces alterations in the salivary glands. The investigation of mechanisms that can reduce the impact of malnutrition on the defenses of the organism is of the utmost important and interest to public health. The aim of this study is to evaluate the effect of low-level laser on the saliva of children aged 1 to 5 years with energy-protein malnutrition.

Authors : Vieira Karlla Almeida, Bastos Clarissa Moraes, Vitor Marilya Gabriella Correia, Deana Alessandro Melo, Fernandes Kristianne Porta Santos, Rodrigues Maria Fernanda Setúbal Destro, Pavesi Vanessa Christina Santos, Bussadori Sandra Kalil,



(5) Serum brain-derived neurotrophic factor level and exercise tolerance complement each other in predicting the prognosis of patients with heart failure.[TOP]

Pubmed ID :29700574
Publication Date : //
Brain-derived neurotropic factor (BDNF) is a myokine that plays a key role in regulating survival, growth, and maintenance of neurons. We investigated whether the serum BDNF level at discharge could predict the prognosis in patients with heart failure (HF). Furthermore, we aimed to examine the relationship between this myokine and exercise tolerance. We prospectively enrolled 94 patients who were hospitalized for worsening HF and had cardiac rehabilitation. At discharge, the serum BDNF level of all patients was measured using a commercial ELISA kit and they underwent a cardiopulmonary exercise test to measure peak oxygen uptake (peak VO). Correlation was not observed between BDNF and peak VO. Kaplan-Meier analysis demonstrated that cardiac death or rehospitalization owing to worsening HF was significantly higher in the low BDNF group (p = 0.023). The combination of peak VO and BDNF levels led to the identification of subgroups with significantly different probabilities of events (p = 0.005). In particular, in the low BDNF and low peak VO group, the frequency of rehospitalization within half a year after discharge was much higher than that in other groups. Multivariate analysis found BDNF as an independent factor of adverse events (hazard ratio 0.956; 95% confidence interval 0.911-0.999; p = 0.046). The serum BDNF level at discharge may be a useful biomarker of the prognosis in patients with HF. Furthermore, combining BDNF and peak VO may be useful for predicting early cardiac events.

Authors : Shibata Atsushi, Hanatani Akihisa, Izumi Yasukatsu, Kitada Ryoko, Iwata Shinichi, Yoshiyama Minoru,



(6) Design and construction of a new recombinant fusion protein (2b2t+EPC1) and its assessment for serodiagnosis of cystic echinococcosis.[TOP]

Pubmed ID :29696723
Publication Date : //
The immunodiagnostic tests for cystic echinococcosis (CE) are mostly serological tests based on ELISA that use hydatid cyst antigens for primary screening because of its simple preparation and availability. The challenge to develop new serological methods (as compared to those based on the hydatid cyst fluid antigens) to meet the gold standard remains. Appropriate sources of antigenic material are necessary for application to improve the efficacy of immunodiagnostic tests at a population level. In the current study, a fusion protein containing the coding sequence of antigen B2t and two sequences of EPC1 antigen with some modifications was reconstructed. Using bioinformatics tools, these sequences were joined together by applying the sequence of a rigid α-helix-forming linker to obtain an appropriate structure of a fusion protein. Synthetic recombinant fusion protein was expressed using pET28a as a vector and evaluated by indirect ELISA test for sera from patients with hepatic CE and other parasitic infections. The sensitivity of the fusion protein was lower (88.46%) than the available ELISA kit (96.15%). However, the differences in sensitivity were not statistically significant as compared to the recombinant fusion peptide with the commercial kit (p = 0.269). The specificity of the recombinant fusion protein (95.45%) was not significantly lower than the commercial kit (96.59%; p = 1.000). Moreover, surprisingly there was no difference in the cross-reactivity values of performance between the recombinant-ELISA and commercial kit. The positive and negative predictive values of the recombinant antigen were achieved as 92% and 93.33%, respectively, while for the commercial kit, they were obtained as 94.33% and 97.70%, respectively. In conclusion, as an early evaluation of these antigens the performance of our recombinant fusion protein in ELISA is relatively promising. Although, it seemed that this peptide with specific antigenic epitopes might be more appropriate for the serological evaluation of CE by use of bioinformatics tools, our findings showed that cross-reactions and a negative reaction could occur in clinical performance. This fusion protein may have utility for diagnosis in humans, but further evaluation is needed using the WHO ultrasound classification for CE.

Authors : Fathi Saeid, Jalousian Fatemeh, Hosseini Seyed Hossein, Najafi Ali, Darabi Enayat, Koohsar Faramarz,



(7) In Vivo Two-Photon Fluorescence Imaging of the Activity of the Inflammatory Biomarker LTAH in a Mouse Pneumonia Model.[TOP]

Pubmed ID :29682967
Publication Date : //
Severe atmospheric haze caused by industrial pollution has severely affected human health and led to the increasing incidence of cardiopulmonary diseases, including pneumonia. Conventional methods for diagnosis of pneumonia are complicated and tedious, and current clinical imaging techniques might cause organ injuries to some extent. Therefore, an accurate, fast, and intact imaging method must be developed to diagnose pneumonia in the early stages. In this study, we propose a new two-photon fluorescence probe, named as ASPC, for detection of the activity of the inflammatory biomarker LTAH through specific recognition and cleavage of amides containing the unnatural amino acid l-AspBzl. The activity of LTAH in the lung tissues of mice was rapidly and accurately monitored for the first time and could be an indicator for diagnosis of pneumonia. The severity of pneumonia in mice caused by haze particulate was determined through imaging the activity of LTAH as biomarker and confirmed using a commercial ELISA kit of interleukin-1β. This work provides a promising method for clinical detection of pneumonia and for screening specific depressors of LTAH as potential drug candidates.

Authors : Wang Hui, Xue Ke, Li Ping, Yang Yuyun, He Zixu, Zhang Wei, Zhang Wen, Tang Bo,



(8) Photodynamic therapy induces antifibrotic alterations in primary human vocal fold fibroblasts.[TOP]

Pubmed ID :29668038
Publication Date : //
Photodynamic therapy (PDT) is a promising treatment modality for laryngeal dysplasia, early-stage carcinoma, and papilloma, and was reported to have the ability to preserve laryngeal function and voice quality without clinical fibrotic response. We aimed to investigate the mechanism behind the antifibrotic effects of PDT on primary human vocal fold fibroblasts (VFFs) in vitro.

Authors : Zhang Chi, Wang Jiajia, Chou Adriana, Gong Ting, Devine Erin E, Jiang Jack J,



(9) Cofilin-2 Acts as a Marker for Predicting Radiotherapy Response and Is a Potential Therapeutic Target in Nasopharyngeal Carcinoma.[TOP]

Pubmed ID :29664897
Publication Date : //
BACKGROUND The purpose of this study was to determine whether cofilin-2 could serve as a protein marker for predicting radiotherapy response and as a potential therapeutic target in nasopharyngeal carcinoma (NPC). MATERIAL AND METHODS Cofilin-2 protein levels in serum and tissue samples from patients with NPC were assessed by sandwich ELISA and IHC. In vitro, cofilin-2 levels in CNE-2R cells were significantly higher than those of CNE-2 cells. Meanwhile, CNE-2R cells were silenced for cofilin-2 to obtain a stable cofilin-2-RNAi-LV3 cell line. Then, cell proliferation, radiosensitivity, invasion and migration abilities, cell cycle, and apoptosis were evaluated by Cell Counting Kit 8 assay (CCK-8), flow cytometry (FCM), clone formation assay, and in vitro. RESULTS The secreted levels of the cofilin-2 protein in radioresistant NPC patients were significantly higher than those of radiosensitive cases. After cofilin-2 knockdown in nasopharyngeal carcinoma CNE-2R cells, proliferation was decreased, while apoptosis and radiosensitivity were enhanced; cell cycle distribution was altered, and the transplanted tumors in nude mice grew significantly less. CONCLUSIONS Overall, our findings suggest that cofilin-2 acts as a marker for predicting radiotherapy response and is a potential therapeutic target in nasopharyngeal carcinoma.

Authors : Yu Bin-Bin, Lin Guo-Xiang, Li Ling, Qu Song, Liang Zhong-Guo, Chen Kai-Hua, Zhou Lei, Lu Qi-Teng, Sun Yong-Chu, Zhu Xiao-Dong,



(10) Relationship between serum osteoprotegerin and vascular calcifications in hemodialysis patients.[TOP]

Pubmed ID :29622969
Publication Date : //
Uremia is a vasculopathic process, and both cardiac calcification and vascular calcification seen from the early stages of chronic kidney disease. Osteoprotegerin could play a crucial role in atherosclerotic plaque formation, maturation and calcification. The goal of this study was to determine the relationship of serum osteoprotegerin with vascular calcification in patients with end stage kidney disease who were maintained on regular hemodialysis.

Authors : El Baz Tarek Z, Khamis Osama A, El-Shehaby Amal, Chahine Hussein, Alaa Al-Din Ahmed Ahmad, Alsawasany Mostafa A,