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ELISA Kit for Early Prostate Cancer Antigen 2 (EPCA2)

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[#E84215Hu] ELISA Kit for Early Prostate Cancer Antigen 2 (EPCA2)


E84215Hu | ELISA Kit for Early Prostate Cancer Antigen 2 (EPCA2) , 96T/Kit
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(1) Cancer stem cell-like characteristics and telomerase activity of the nasopharyngeal carcinoma radioresistant cell line CNE-2R.[TOP]

Pubmed ID :30105829
Publication Date : //
The radioresistance of nasopharyngeal carcinoma (NPC) may be related to cancer stem cells (CSCs), and the characteristics of CSCs may be maintained by telomerase activity. In this study, we explored the CSC-like characteristics and telomerase activity of the NPC radioresistant cell line CNE-2R. This work provides a foundation for future studies on stem cell-targeted therapies by targeting the radioresistance of NPC. The expression of stem cell-related genes/proteins and the hTERT gene/protein in CNE-2R and its parent radiosensitive cell line CNE-2 were detected using qPCR/Western Blot. Label-retaining cells (LRCs) were detected through immunocytochemistry, and telomerase activity was detected using a PCR-ELISA kit. CD133 expression was detected with flow cytometry. CNE-2R-CD133+ and CNE-2R-CD133- cells were separated with magnetic-activated cell sorting. The proliferation and tumorigenesis capacities of CNE-2R-CD133+, CNE-2R-CD133-, and CNE-2R cells were compared with a CCK-8 assay, sphere formation assay, and an in vivo experiment. Our results showed that the expression of stem cell-related genes and the hTERT gene in CNE-2R cells was higher than those in CNE-2 cells. Similarly, the expression of stem cell-related proteins and the hTERT protein in CNE-2R cells was markedly higher than those in CNE-2 cells. The proportion of LRCs in CNE-2R and CNE-2 cells was (3.10 ± 0.63%) vs (0.40 ± 0.35%; P < 0.001), respectively. Telomerase activity in CNE-2R cells was remarkably higher than that in CNE-2 cells. Flow cytometry suggested that the CD133 positive rates in CNE-2R and CNE-2 cells were (2.49 ± 0.56%) vs (0.76 ± 0.25%; P = 0.008), respectively. Meanwhile, the proliferation capacity, tumorigenesis capacity, and telomerase activity of CNE-2R-CD133+ cells were notably higher than those of CNE-2R-CD133- and CNE-2R cells. Collectively, CNE-2R displayed CSC-like characteristics; our results also showed that CNE-2R cells, especially the sorted CSCs, had high telomerase activity levels.

Authors : Chen Kai-Hua, Guo Ya, Li Ling, Qu Song, Zhao Wei, Lu Qi-Teng, Mo Qi-Yan, Yu Bin-Bin, Zhou Lei, Lin Guo-Xiang, Sun Yong-Chu, Zhu Xiao-Dong,

(2) Serum Cathelicidin level as a Marker for Diabetic Nephropathy in Patients with Type 1 Diabetes mellitus.[TOP]

Pubmed ID :30091508
Publication Date : //
The aim of the study was to evaluate the relationship between serum cathelicidin level and diabetic nephropathy (DN) in patients with type 1 diabetes mellitus (T1DM).

Authors : El-Ashmawy Hazem M, Ahmed Azza M,

(3) Glycogen Phosphorylase BB: A more Sensitive and Specific Marker than Other Cardiac Markers for Early Diagnosis of Acute Myocardial Infarction.[TOP]

Pubmed ID :30072837
Publication Date : //
Cardiac markers are used to evaluate functions of heart. However, there are no satisfactory cardiac biomarkers for the diagnosis of acute myocardial infarction (AMI) within 4 h of onset of chest pain. Among novel cardiac markers, glycogen phosphorylase BB (GPBB) is of particular interest as it is increased in the early hours after AMI. The present study was conducted with the objective to find out the sensitivity and specificity of GPBB over other cardiac markers i.e. myoglobin and CKMB in patients of AMI within 4 h after the onset of chest pain. The study includes 100 AMI patients and 100 normal healthy individuals as controls. In all the cases and controls, serum GPBB and myoglobin concentrations were measured by ELISA where as CK-MB was measured by diagnostic kit supplied by ERBA. The sensitivity and specificity of glycogen phosphorylase BB (GPBB) were greater than CK-MB and myoglobin in patients of AMI within 4 h after the onset of chest pain. Hence, glycogen phosphorylase BB (GPBB) can be used as additional biomarker for the early diagnosis of AMI.

Authors : Singh Neelima, Rathore Vedika, Mahat Roshan Kumar, Rastogi Puneet,

(4) Top-down and sensitive indium oxide nanoribbon field effect transistor biosensor chips integrated with on-chip gate electrodes toward point of care applications.[TOP]

Pubmed ID :30020085
Publication Date : //
We report a scalable, uniform, and sensitive top-down fabricated indium oxide (InO) nanoribbon biosensor platform with integrated on-chip gate electrodes using two photolithographic masks. The purpose of this on-chip gate electrode is to control the operational point of the sensor during biomolecular detection replacing the cumbersome external Ag/AgCl electrode. It exhibits excellent capability in gating transistors in an aqueous condition and high stability during the sensing experiment, which is similar to the Ag/AgCl electrode. Its compactness increases the portability and pushes this platform toward a practical use. To demonstrate its capability for detection of biomolecules, we combine this platform with the electronic enzyme-linked immunosorbent assay (ELISA) technique to amplify the signal and to bypass limitation of the Debye screening effect from high salt concentration of physiological samples. Troponin I, a cardiac marker for diagnosis of acute myocardial infarction (AMI), was selected as the target molecule in this study. The InO nanoribbon device offers a high response of 30% toward 0.1 pg ml troponin I concentration and a lower detection limit than that of the commercial ELISA kit on the market by five orders of magnitude. The total assay time from the sample collection to the data acquisition is about 45 min, which is within the constraint of the emergency care application. With the demonstrated sensitivity, uniformity, scalability, quick turn-around time and ability to be integrated, our InO nanoribbon biosensor platform has high potential toward clinical tests for early diagnosis of AMI.

Authors : Aroonyadet N, Jeamsaksiri W, Wisitsoraat A, Tuantranont A,

(5) Evaluation of serum SLCO1B1 levels and genetic variants of SLCO1B1 rs4149056 and rs2306283 in patients with early and exudative age-related macular degeneration.[TOP]

Pubmed ID :30010042
Publication Date : //
To determine SLCO1B1 rs4149056 and rs2306283 gene polymorphisms and SLCO1B1 serum levels in patients with early and exudative age-related macular degeneration.

Authors : Liutkeviciene Rasa, Vilkeviciute Alvita, Slavinskaite Aiste, Petrauskaite Aiste, Tatarunas Vacis, Kriauciuniene Loresa,

(6) Detection of pregnancy in sheep using an ELISA for pregnancy-specific protein B.[TOP]

Pubmed ID :29958526
Publication Date : //
The early detection of pregnancy and the determination of fetal numbers have economic benefits in sheep production because of the seasonal breeding patterns where missing a breeding opportunity means the loss of one productive year. The purpose of this study was to evaluate the efficacy of the B6-HRP ELISA for ovine pregnancy-specific protein B (oPSPB) measurement in the detection of pregnancy and estimation of fetal numbers in different sheep breeds. BioPRYN® ELISA assay kit was used for the detection of pregnancy in the experimental animals. Ninety-three ewes of three breeds (British Milksheep - BM, Lacaune - L and Transylvanian Racka - TR), each from three farms in Hungary, were included in the study. BM and L ewes were artificially inseminated (AI). Thirty-five days after AI, all ewes were examined by transabdominal ultrasound. The TR flock was mated naturally over a six-week period. At the end of the mating period, the ewes were similarly examined by ultrasound. Blood samples were taken from all pregnant ewes twice (35 and 65 days after AI), and serum samples were assayed by the BioPRYN test. It can be concluded that the detection of serum PSPB by ELISA is a much easier, safer, less expensive and highly accurate method for the detection of ovine pregnancy. Although some breed-related differences were detectable at 35 and 65 days post breeding, no differences in oPSPB levels were found in pregnant ewes carrying different numbers of fetuses.

Authors : Milisits-Németh Tímea, Balogh Orsolya Gabriella, Egerszegi István, Kern László, Sasser R Garth, Gábor György,

(7) Development of Time-Resolved Fluoroimmunoassay for Detection of Cylindrospermopsin Using Its Novel Monoclonal Antibodies.[TOP]

Pubmed ID :29933618
Publication Date : //
Cylindrospermopsin (CYN) is a cyanotoxin that is of particular concern for its potential toxicity to human and animal health and ecological consequences due to contamination of drinking water. The increasing emergence of CYN around the world has led to urgent development of rapid and high-throughput methods for its detection in water. In this study, a highly sensitive monoclonal antibody N8 was produced and characterized for CYN detection through the development of a direct competitive time-resolved fluorescence immunoassay (TRFIA). The newly developed TRFIA exhibited a typical sigmoidal response for CYN at concentrations of 0.01⁻100 ng mL, with a wide quantitative range between 0.1 and 50 ng mL. The detection limit of the method was calculated to be 0.02 ng mL, which is well below the guideline value of 1 μg L and is sensitive enough to provide an early warning of the occurrence of CYN-producing cyanobacterial blooms. The newly developed TRFIA also displayed good precision and accuracy, as evidenced by low coefficients of variation (4.1⁻6.5%). Recoveries ranging from 92.6% to 108.8% were observed upon the analysis of CYN-spiked water samples. Moreover, comparison of the TRIFA with an ELISA kit through testing 76 water samples and 15 cultures yielded a correlation ² value of 0.963, implying that the novel immunoassay was reliable for the detection of CYN in water and algal samples.

Authors : Lei Lamei, Peng Liang, Yang Yang, Han Bo-Ping,

(8) An improved, rapid competitive ELISA using a novel conserved 3B epitope for the detection of serum antibodies to foot-and-mouth disease virus.[TOP]

Pubmed ID :29916768
Publication Date : //
The highly contagious foot-and-mouth disease virus (FMDV) afflicts cloven-hoofed animals, resulting in significant costs because of loss of trade and recovery from disease. We developed a sensitive, specific, and rapid competitive ELISA (cELISA) to detect serum antibodies to FMDV. The cELISA utilized a monoclonal blocking antibody specific for a highly conserved FMDV nonstructural 3B epitope, a recombinant mutant FMDV 3ABC coating protein, and optimized format variables including serum incubation for 90 min at 20-25°C. Samples from 16 animals experimentally infected with one FMDV serotype (A, O, Asia, or SAT-1) demonstrated early detection capacity beginning 7 d post-inoculation. All samples from 55 vesicular stomatitis virus antibody-positive cattle and 44 samples from cloven-hoofed animals affected by non-FMD vesicular diseases were negative in the cELISA, demonstrating 100% analytical specificity. The diagnostic sensitivity was 100% against sera from 128 cattle infected with isolates of all FMDV serotypes, emphasizing serotype-agnostic results. Diagnostic specificities of U.S. cattle ( n = 1135) and swine ( n = 207) sera were 99.4% and 100%, respectively. High repeatability and reproducibility were demonstrated with 3.1% coefficient of variation in percent inhibition data and 100% agreement using 2 kit lots and 400 negative control serum samples, with no difference between bench and biosafety cabinet operation. Negative results from vaccinated, uninfected cattle, pig, and sheep sera confirmed the DIVA (differentiate infected from vaccinated animals) capability. This rapid (<3 h), select agent-free assay with high sensitivity and specificity, DIVA capability, and room temperature processing capability will serve as a useful tool in FMDV surveillance, emergency preparedness, response, and outbreak recovery programs.

Authors : Chung Chungwon J, Clavijo Alfonso, Bounpheng Mangkey A, Uddowla Sabena, Sayed Abu, Dancho Brooke, Olesen Ian C, Pacheco Juan, Kamicker Barbara J, Brake David A, Bandaranayaka-Mudiyanselage Carey L, Lee Stephen S, Rai Devendra K, Rieder Elizabeth,

(9) High-dose HOOK effect in urinary DcR2 assay in patients with chronic kidney disease.[TOP]

Pubmed ID :29879421
Publication Date : //
Urinary DcR2 (uDcR2) is a biomarker for the early detection the tubulointerstitial injury (TII) in patients with chronic kidney disease (CKD), but the high-dose hook effect may lead to falsely low or even negative results when using an enzyme-linked immunosorbent assay (ELISA). This study aimed to investigate if the high-dose hook effect exists with ELISA testing, and to uncover a potential approach for reducing this effect.

Authors : Chen Jia, Chen Ke-Hong, Wang Li-Ming, Zhang Wei-Wei, Feng Lei, Dai Huan-Zi, He Ya-Ni,

(10) Studying the frequency of aberrant DNA methylation of APC, P14, and E-cadherin genes in HCV-related hepatocarcinogenesis.[TOP]

Pubmed ID :29865038
Publication Date : //
Data about the molecular pathogenesis of hepatitis C-related hepatocellular carcinoma (HCC) are still challenging.

Authors : Mekky Mohamed A, Salama Rgaa H, Abdel-Aal Mahmoud F, Ghaliony Mohamed A, Zaky Saad,